20th World Congress on Ultrasound in Obstetrics and GynecologyPoster abstracts and consecutive daily profiles were normal. Serial US exams were performed later; estimated fetal weight was in the normal range and IUGR was excluded. The AFI measurements were between 3 and 9 cm alternately ( Figure 1). It was noticed that cord was present in every pocket. At 35+5 gestational age due to AFI-3 cm, and a decision was made to induce labor and a Foley catheter was inserted. The woman went into labor, a rupture of membranes was performed with normal amount of amniotic fluid. The patient delivered a baby girl, 2330 g, with Apgar scores of 9 and 10 at 1 and 5 minutes respectively. A long cord of over 120 cm was measured.Supporting information can be found in the online version of this abstract.
P11: PELVIC PAIN AND REPRODUCTIVE MEDICINE
P11.01 Preimplantation diagnosis of monogenic diseases in GENNET
GENNET, Prague, Czech RepublicObjectives: In 2007 we completed the first in vitro fertilization cycle (IVF) followed by preimplantation genetic diagnostics (PGD) of cystic fibrosis. Since than, we have performed over 65 cycles with scheduled PGD for 27 different monogenic diseases all types of indication: X-linked, autosomal recessive or autosomal dominant. The list of diagnosis where we can offer PGD is being continually extended. Methods: In our center we use genetic haplotyping technique by multiplex PCR on products of MDA (multiple displacement amplification) from 1 blastomere biopsied from the cleavage-stage embryo. In rare cases of unambiguous results from the blastomere, analysis can be repeated from trofoectoderm and embryo is still managed to be transferred on the day 5. Results: Up to now, we have accomplished 65 IVF cycles with PGH. On average 7-8 embryos were biopsied in one IVF cycle, as preferably mild stimulation protocols were applied. Success rate of the procedure (calculated on the fetal heart beat pregnancy) reached 63% on embryo transfer and 50% per completed cycle. In 2009 we completed accreditation of the method by ISO regulation 15189: 2007 and successfully participated in pilot EQA organized by UK NEQAS. Conclusions: PGD represents an alternative within prenatal diagnosis services and so offers a chance for many couples in risk of genetic disease to have a healthy baby and to avoid the risk termination of the pregnancy from genetic reasons or birth of affected child. Currently available PGD technology can help eliminate some genetic diseases in the future.
P11.02The appearance of the endometrium at saline contrast sonohysterography in the luteal phase L. Jokubkiene, P. Sladkevicius, L. I. Valentin
Department of Obstetrics and Gynecology, Skåne University Hospital, Malmö, SwedenObjectives: It is not known how often endometrial folds are seen at SCSH in the luteal phase and if folds are difficult to distinguish from focal lesions. Our aim was to describe the ultrasound morphology of the endometrium at SCSH in the luteal phase of the menstrual cycle. Methods: 23 women 20-38 years old with regular menstrual...