Comparison of toxicity and transfection efficiency of amphiphilic block copolymers and polycationic polymers in striated muscles

Abstract

The present study highlights the interest in amphiphilic carriers for promoting DNA transfection in vivo. Gaining new insights into the properties of these vectors should allow their optimization.

Degradable gene delivery systems based on Pluronics-modified low-molecular-weight polyethylenimine: preparation, characterization, intracellular trafficking, and cellular distribution

“…21 The cytotoxicity of cationic polymers is thought to be a result of the multiple attachment of PEI to the cell surface and the membrane-damaging effects. 27 The results of this study show that PEI macromolecules can be conjugated with Pluronic and that PPs are degradable under physiological conditions. The degradation products of the polymer are low-molecular-weight PEI and Pluronics, which are practically nontoxic and are rapidly excluded out of the cell nucleus and the cytoplasm or eliminated from the body.…”

“…The hydrophilic EO chains extend into water and sterically prevent the complexes from approaching each other, 28 and they can shield the positive surface charge of complexes. 27 Therefore, the EO chain can reduce toxicity and improve the colloidal stability of the polymer/DNA complexes as well. 26 Also, the PO chain is believed to enhance lipophilic complexes, which possibly possess the ability of interacting with biological membranes and enhancing transport of the complexes into cells.…”

Polymer gene delivery vectors encapsulated in thermally sensitive bioreducible shell

“…The main drawback of the first and most widely used cationic homopolymers-polyethyleneimine (PEI) and poly(L-lysine) is their relatively high cytotoxicity. 3,4 The recent advances in macromolecular engineering allow the design and synthesis of more complex polymer architectures. 5 Thus, numerous copolymers of various architectures with cationic segments and potential application in gene delivery systems were synthesized.…”

Morphological Changes in Paraurethral Area after Introduction of Tissue Engineering Construct on the Basis of Adipose Tissue Stromal Cells

“…However, their effi ciency is not always suffi cient (treatment can be associated with complications). The methods based on the use of preparation creating additional volume in the site of their introduction and aimed at attaining pressure equilibrium between the urinary bladder and urethra have some advantages (biomaterials forming additional artifi cial uncontrollable sphincter increasing partial pressure in the urethra are introduced para-or periurethrally [15]). …”

“…When choosing the optimal carrier for TEC we excluded many synthetic preparations [5,6,15], preparations on the basis of organic acid polyesters [14], and gels on the basis of collagen and hyaluronic acid [6]. Gelatin sponge was used as a carrier for TEC [10,18].…”