Comparison of Thyroid Transcription Factor-1 and Hepatocyte Antigen Immunohistochemical Analysis in the Differential Diagnosis of Hepatocellular Carcinoma, Metastatic Adenocarcinoma, Renal Cell Carcinoma, and Adrenal Cortical Carcinoma

Wieczorek, Tad J.; Pinkus, Jack L.; Glickman, Jonathan N.; Pinkus, Geraldine S.

doi:10.1309/kkdp-mnd2-w794-p9eh

Cited by 72 publications

(60 citation statements)

References 22 publications

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“…16,24 Many authors reported on the immunoreactivity spectrum of HepPar1 in formalin-fixed tissue from a variety of neoplasms. Lugli et al 24 found positivity in 35 of 48 HCCs (73%), Chu et al 25 in 88 of 96 HCCs (92%), Lee et al 26 in 60 of 75 HCCs (80%), and Wieczorek et al 27 in 50 of 76 HCCs (66%). In our study, HepPar1 was positive in all HCCs, as Siddiqui et al 28 have also reported.…”

Section: Discussionmentioning

confidence: 97%

Immunocytochemical diagnosis of hepatocellular carcinoma and identification of carcinomas of unknown primary metastatic to the liver on fine-needle aspiration cytologies

Onofre

Pomjanski

Buckstegge

et al. 2007

Cancer

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We successfully synthesized an exfoliated styrene–butadiene–styrene triblock copolymer (SBS)/montmorillonite nanocomposite by anionic polymerization. Gel permeation chromatography showed that the introduction of organophilic montmorillonite (OMMT) resulted in a small high‐molecular‐weight fraction of SBS in the composites, leading to a slight increase in the weight‐average and number‐average molecular weights as well as the polydispersity index. The results from 1H‐NMR revealed that the introduction of OMMT almost did not affect the microstructure of the copolymer when the OMMT concentration was lower than 4 wt %. Transmission electron microscopy and X‐ray diffraction showed a completely exfoliated nanocomposite, in which both polystyrene and polybutadiene blocks entered the OMMT galleries, leading to the dispersion of OMMT layers on a nanoscale. The exfoliated nanocomposite exhibited higher thermal stability, glass‐transition temperature, elongation at break, and storage modulus than pure SBS. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci, 2006

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Section: Discussionmentioning

confidence: 97%

Immunocytochemical diagnosis of hepatocellular carcinoma and identification of carcinomas of unknown primary metastatic to the liver on fine-needle aspiration cytologies

Onofre

Pomjanski

Buckstegge

et al. 2007

Cancer

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“…16 This antibody has shown some nonspecific cytoplasmic staining and suspected to crossreact with a cytoplasmic protein in liver cells. 17 In the current study, we also used another monoclonal antibody, the SPT24 clone, to detect the expression of TTF-1. In total, 10% of lung-metastatic colorectal adenocarcinoma and 5% of primary colorectal adenocarcinomas were found positive, respectively, these results dramatically differ from the literature, which showed no positive cases in a total of 131 primary colonic carcinomas and 52 metastatic carcinomas in the lung (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Variable sensitivity and specificity of TTF-1 antibodies in lung metastatic adenocarcinoma of colorectal origin

et al. 2005

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Thyroid transcription factor-1 (TTF-1) is considered as a reliable marker for differential diagnosis in distinguishing primary adenocarcinomas of the lung from extrathoracic origins. We previously reported the first case of lung metastasis of colorectal origin, with nuclear expression of TTF-1. As most previous studies were performed on series of extrathoracic primary tumors, we raised the question of a possible role of lung microenviroment in TTF-1 expression. We investigated the rate of TTF-1 expression in lung metastases of extrathoracic adenocarcinomas and compared results of immunohistochemistry performed with different primary antibodies. Two different clones of antibodies (8G7G1/1 from Dako, SPT24 from Novocastra) raised against TTF-1 were used on 56 lung-metastatic malignant tumors, 41 from colorectal origin. A series of primary colorectal (90 cases) and primary pulmonary adenocarcinomas (86 cases) were also investigated. Four of 41 (10%) lung metastases of colorectal adenocarcinomas displayed a nuclear staining for TTF-1 with SPT24 clone. Three of the four positive cases displayed similar nuclear staining in primary and/or other extrathoracic metastatic sites as well as four of 90 (5%) primary colorectal adenocarcinomas, ruling out the role of lung microenvironment. None of them was positive with 8G7G1/1 clone. Sensitivity between two sets of antibodies was compared in 86 primary pulmonary adenocarcinomas. Nuclear staining was detected in 72 cases (84%) with Novocastra's antibody and 56 cases (65%) with Dako's. Significant discordance was observed (Po 0.01). These results suggest that the diagnostic virtue of TTF-1 detection depends on the used antibody's clone. The SPT24 clone seems to have a stronger affinity for TTF-1 protein but may lead to a few positive colorectal adenocarcinomas. Keywords: TTF-1; lung; carcinoma; metastasis; colorectal Thyroid transcription factor-1 (TTF-1) is a tissuespecific transcription factor expressed in the epithelial cells of thyroid and lung (type II pneumocytes and Clara cells). Carcinomas arising from lung and thyroid also show frequent TTF-1 expression. 1,2 As the lung is one of the most common sites of metastasis, TTF-1 is considered as a reliable marker to distinguish between primary lung carcinoma and lung metastasis, especially when dealing with an adenocarcinoma or a large-cell carcinoma. [3][4][5][6] It is also considered as a reliable marker in the differential diagnosis between pleural localization of lung origin carcinoma and malignant mesothelioma. 7 Two main commercial available clones of monoclonal antibodies have been raised against TTF-1 for immunohistochemical use, 8G7G1/1 and SPT24, their diagnostic value in determining the lung or thyroid origin of adenocarcinoma has not yet been compared. Most of the studies show the high specificity of TTF-1 detection and have been published used the 8G7G1/1 clone. We previously reported one case of lung metastasis of colorectal origin, which showed a focal nuclear staining with the anti-TTF-1 antibody (clone SPT24)...

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“…Most adenocarcinomas are positive for carcinoembryonic antigen, epithelial membrane antigen and CD15 while adrenocortical carcinomas are negative [7,27]. Adrenocortical carcinomas are also negative with the renal cell carcinoma (RCC) and hepatocyte antibodies [19,31] (Table 1). …”

Section: Other Markersmentioning

confidence: 99%

Immunohistochemical Characteristics of Adrenocortical Carcinoma: An Overview

DeLellis

Shin

2003

Acta Histochem. Cytochem

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Immunohistochemistry has played a major role in the practice of surgical pathology over the past three decades. These techniques have been of particular value in the diagnosis and classification of tumors of the adrenal cortex. The intermediate filament phenotype of adrenocortical carcinomas includes positivity for vimentin (80-100%), cytokeratin (40-50%) and neurofilament proteins (50%). Other markers that have proven useful for the diagnosis of these tumors include melan A (A103), alpha inhibin, synaptophysin, calretinin, D11 and the nuclear protein adrenal 4 binding protein.The judicious use of these markers permits the distinction of adrenocortical carcinomas from other tumor types and may also be of value in the identification of the functional characteristics of these neoplasms.

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Comparison of Thyroid Transcription Factor-1 and Hepatocyte Antigen Immunohistochemical Analysis in the Differential Diagnosis of Hepatocellular Carcinoma, Metastatic Adenocarcinoma, Renal Cell Carcinoma, and Adrenal Cortical Carcinoma

Cited by 72 publications

References 22 publications

Immunocytochemical diagnosis of hepatocellular carcinoma and identification of carcinomas of unknown primary metastatic to the liver on fine-needle aspiration cytologies

Immunocytochemical diagnosis of hepatocellular carcinoma and identification of carcinomas of unknown primary metastatic to the liver on fine-needle aspiration cytologies

Variable sensitivity and specificity of TTF-1 antibodies in lung metastatic adenocarcinoma of colorectal origin

Immunohistochemical Characteristics of Adrenocortical Carcinoma: An Overview

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