Comparison of three immunosensor methods (surface plasmon resonance, screen-printed and classical amperometric immunosensors) for immunoglobulin G determination in human serum and animal or powdered milks

Tomassetti, M.; Martini, Elisabetta; Campanella, Luigí; Favero, Gabriele; Carlucci, Luciano; Mazzei, Franco

doi:10.1016/j.jpba.2012.03.020

Cited by 17 publications

(9 citation statements)

References 33 publications

(36 reference statements)

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“…Microscopic observation revealed that IgG promoted aggregation of S. aureus in LB, whereas no significant increase in aggregation was observed in media supplemented with lactoferrin or BSA. The mean IgG concentration in healthy bovine milk samples is 0.45-1.34 mg ml −1 , significantly smaller than the value in mastitic milk, 3-15 mg ml −1 [22]. Based on these observations, we hypothesized that the size of S. aureus aggregates changes according to the concentration of IgG in the bovine udder.…”

Section: Aggregation Of S Aureus In the Presence Of Milk Componentsmentioning

confidence: 93%

IgG-dependent aggregation of Staphylococcus aureus inhibits bacteriophage attack

Tanji

Tanaka

Tani

et al. 2015

Biochemical Engineering Journal

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Section: Aggregation Of S Aureus In the Presence Of Milk Componentsmentioning

confidence: 93%

IgG-dependent aggregation of Staphylococcus aureus inhibits bacteriophage attack

Tanji

Tanaka

Tani

et al. 2015

Biochemical Engineering Journal

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“…The use of the extended spacer arm greatly improves the interaction between extravidin and the biotinylated macromolecule, thus overcoming the steric hindrance present at the biotin binding sites of extravidin [ 32 ]. Detailed descriptions of this technique were reported in previous papers [ 23 , 24 , 25 , 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…Our research group has worked on inhibition biosensors, in particular tyrosinase biosensors, designed to analyse triazine and benzotriazine compounds [ 19 , 20 ]. In recent years, our team has investigated the development of conventional immunosensors [ 21 , 22 , 23 , 24 ], or screen-printed methods [ 25 , 26 ] above all because they were found to be more selective for the different classes of pesticide. In this framework the aim of the present research was to compare the analytical features of a new SPR device with two conventional or screen-printed amperometric immunosensor devices.…”

Section: Introductionmentioning

confidence: 99%

A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices

Tomassetti

Martini

Campanella

et al. 2015

Sensors

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A detailed comparison was made of the analytical features of a new Surface Plasmon Resonance (SPR) immunodevice for triazine pesticide determination with those of two other amperometric (conventional and screen-printed) immunosensors and the advantages and disadvantages of the SPR method were thoroughly investigated. For conventional amperometric and screen-printed devices, “competitive” assays were used; conversely, the SPR transduction technique allowed a “direct” measurement format to be used. As far as the main analytical data are concerned, the SPR method does not seem to offer substantial advantages. Nevertheless the measurement time is much shorter and the measurement itself much easier to perform. Lastly several applications and recovery tests were carried out on bovine milk samples, before and after spiking, to check for triazine pesticides in the samples, obtaining satisfactory results.

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“…Bioaffinity sensors with a pre-formed metastable ligand-receptor complex that are homologus to the enzyme immunosensor have been used in the determination of thyroxine (T4), biotin and insulin. [404142]…”

Section: Types Of Cell-based Biosensorsmentioning

confidence: 99%

Biosensors in clinical chemistry: An overview

et al. 2014

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Biosensors are small devices that employ biological/biochemical reactions for detecting target analytes. Basically, the device consists of a biocatalyst and a transducer. The biocatalyst may be a cell, tissue, enzyme or even an oligonucleotide. The transducers are mainly amperometric, potentiometric or optical. The classification of biosensors is based on (a) the nature of the recognition event or (b) the intimacy between the biocatalyst and the transducer. Bioaffinity and biocatalytic devices are examples for the former and the first, whereas second and third generation instruments are examples for the latter. Cell-based biosensors utilizing immobilized cells, tissues as also enzyme immunosensors and DNA biosensors find variegated uses in diagnostics. Enzyme nanoparticle-based biosensors make use of small particles in the nanometer scale and are currently making a mark in laboratory medicine. Nanotechnology can help in optimizing the diagnostic biochips, which would facilitate sensitive, rapid, accurate and precise bedside monitoring. Biosensors render themselves as capable diagnostic tools as they meet most of the above-mentioned criteria.

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Comparison of three immunosensor methods (surface plasmon resonance, screen-printed and classical amperometric immunosensors) for immunoglobulin G determination in human serum and animal or powdered milks

Cited by 17 publications

References 33 publications

IgG-dependent aggregation of Staphylococcus aureus inhibits bacteriophage attack

IgG-dependent aggregation of Staphylococcus aureus inhibits bacteriophage attack

A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices

Biosensors in clinical chemistry: An overview

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