IgG-dependent aggregation of Staphylococcus aureus inhibits bacteriophage attack

Tanji, Yasunori; Tanaka, Airi; Tani, Kaori; Kurimoto, Miki; Miyanaga, Kazuhiko

doi:10.1016/j.bej.2015.01.007

Cited by 10 publications

(5 citation statements)

References 28 publications

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“…It was first demonstrated in vitro that S. aureus was reduced to below the detection limit by the phage cocktail both in media as well as in raw milk, regardless of IgG concentration. This latter is a key observation as it differs from the results of several previous studies ( Das and Marshall, 1967 ; O’Flaherty et al, 2005a ; Gill et al, 2006b ; Tanji et al, 2015 ) which reported a either a lack of or a significant reduction of phage activity in raw milk or its components. These authors attributed this observation to either whey protein binding of the bacterial cells or bacterial aggregation, purportedly driven by IgG lowering the adsorption rate of phage ( Tanji et al, 2015 ).…”

Section: Discussioncontrasting

confidence: 99%

“…This latter is a key observation as it differs from the results of several previous studies ( Das and Marshall, 1967 ; O’Flaherty et al, 2005a ; Gill et al, 2006b ; Tanji et al, 2015 ) which reported a either a lack of or a significant reduction of phage activity in raw milk or its components. These authors attributed this observation to either whey protein binding of the bacterial cells or bacterial aggregation, purportedly driven by IgG lowering the adsorption rate of phage ( Tanji et al, 2015 ). Although bacterial aggregation was not directly examined as a part of this study, CFU counts suggest that it did not generally occur, as increases in IgG concentration would lead to fewer CFU if aggregation took place.…”

Section: Discussioncontrasting

confidence: 99%

“…Biofilm and aggregate formation are complex and multifactorial processes in S. aureus , and variability between strains has been observed by us (data not shown) and others ( Haaber et al, 2012 ; Thammavongsa et al, 2015 ). In the Tanji report, a different bovine mastitis isolate was used, SA003 (NBRC 110649) ( Tanji et al, 2015 ), and O’Flaherty used the S. aureus strains DPC5246 and DPC5645 from the Dairy Products Research Centre culture collection ( O’Flaherty et al, 2005a ). Thus it is possible that the strain used in this study, N305, does not aggregate to the same degree as these other strains.…”

Section: Discussionmentioning

confidence: 99%

“…This strain is a bovine mastitis isolate that has been widely used for experimental infection of dairy cows and mice ( Breyne et al, 2014 ; Peton et al, 2014 ). The phage cocktail was tested in tryptic soy broth (TSB, Becton Dickinson) and raw milk (Organic Pastures) with the addition of bovine IgG (Equitech-Bio) as a potential inhibitor of phage activity as previously reported ( O’Flaherty et al, 2005a ; Gill et al, 2006b ; Tanji et al, 2015 ). The reported IgG concentration in milk varies between studies, however, for healthy milk it has been reported to be 0.45–1.34 mg/mL and for mastitic milk, 3–15 mg/ml ( Tomassetti et al, 2013 ).…”

Section: Methodsmentioning

confidence: 99%

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Efficacy and Safety of a Bovine-Associated Staphylococcus aureus Phage Cocktail in a Murine Model of Mastitis

Breyne

Honaker²,

Hobbs³

et al. 2017

Front. Microbiol.

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Overuse of antibiotics is a major problem in the treatment of bovine mastitis, and antibiotic treatment is frequently non-curative, thus alternative treatments are necessary. The primary aim of this study was to evaluate the efficacy of a purified phage cocktail for treatment of bovine Staphylococcus aureus mastitis in a well-defined mouse model. Candidate phages were selected based on their in vitro performance and subsequently processed into an optimally composed phage cocktail. The highest scoring phages were further tested for efficacy and resistance suppression in broth and raw milk, with and without supplemental IgG. As these in vitro results displayed significant decreases in CFU, the cocktail was purified for testing in vivo. Lactating mice were intramammarily inoculated with S. aureus N305 (ATCC 29740), a clinical bovine mastitis isolate commonly used for experimental infection of dairy cows. The phage cocktail was applied via the same route 4 h post-inoculation. Treated mammary glands were graded for gross pathological appearance and excised for bacterial and phage load quantification as well as histopathology. Observation of gross macroscopic and histopathological changes and CFU quantification demonstrated that the phage cocktail treatment significantly improved mastitis pathology and decreased bacterial counts. Phage PFU quantification indicated that the tested phage cocktail treatment was able to maintain high intramammary phage titers without spreading systemically. The in vivo results complement the in vitro data and support our concept of phage therapy as an innovative alternative or supplementation therapy to antibiotics for the treatment of bovine mastitis.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Efficacy and Safety of a Bovine-Associated Staphylococcus aureus Phage Cocktail in a Murine Model of Mastitis

Breyne

Honaker²,

Hobbs³

et al. 2017

Front. Microbiol.

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“…Phage therapy against mastitis presents many important challenges. Some of the problems encountered are phage stability, inhibitory effects on the cow's immune system and certain thermolabile proteins are present in raw milk that affect phage-cell interaction (O'FLAHERTY et al, 2005;GILL et al, 2006b;TANJI et al, 2015;BARI et al, 2017).…”

Section: Barasuol Et Almentioning

confidence: 99%

In vitro lytic efficiency of Staphylococcus aureus bacteriophages in bacteria from bovine mastitis: a meta-analysis

et al. 2021

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Bacteriophages have been investigated as alternative to the treatment of bacterial infections, including bovine mastitis, in production animals. In this meta-analysis, we evaluated in vitro efficiency of phages of Staphylococcus aureus against S. aureus, which is involved in the etiology of bovine mastitis. Seventeen studies were included and the bacterial lytic activity was extracted using proportion analysis. The lytic efficiency of phages was obtained in this meta-analysis using a random-effects model [significant difference (P<0.05)]. Forest plots were used to graphically represent the efficiency of phages on bacterial isolates. Most phages (e.g., CS1, DW2, ΦSA011, ΦSA012, ΦSA022, ΦSA023, ΦSA024, ΦSA025, ΦSA037, ΦSA038, ΦSA039, ΦSA041, ΦSA042, ΦSA043, ΦSA044, MSA6, Ufv-aur2 to Ufv-aur11, SAH-1, SPW, vB_SauM_JS25, SaPh1 to SaPh6, SA, SANF, SA2, ΦSA012, ΦSA039, phi11, phiIPLA88, phiIPLA35, phiIPLA-RODI, phiIPLA-C1C, SAJK-IND, vBSP-A1, vBSP-A2, STA1.ST29, EB1.ST11, EB1.ST27, Remus, and ISP) were efficiently lytics or infected most S. aureus isolates, demonstrating 80% (P<0.05) lytic efficiency. The phages SA, SANF and SA2, also demonstrated lytic activity or infected the non-Staphylococcus aureus and Macrococcus caseolyticus isolates. In this meta-analysis, we compared and demonstrated the in vitro efficiency and host range of S. aureus phages. Additionally, the phages represent an alternative to be researched to treat bovine mastitis in dairy cattle caused by the prevalent microorganism, S. aureus.

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Bacteriophages and associated endolysins in therapy and prevention of mastitis and metritis in cows: Current knowledge

Zduńczyk

Janowski

2020

Animal Reproduction Science

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IgG-dependent aggregation of Staphylococcus aureus inhibits bacteriophage attack

Cited by 10 publications

References 28 publications

Efficacy and Safety of a Bovine-Associated Staphylococcus aureus Phage Cocktail in a Murine Model of Mastitis

Efficacy and Safety of a Bovine-Associated Staphylococcus aureus Phage Cocktail in a Murine Model of Mastitis

In vitro lytic efficiency of Staphylococcus aureus bacteriophages in bacteria from bovine mastitis: a meta-analysis

Bacteriophages and associated endolysins in therapy and prevention of mastitis and metritis in cows: Current knowledge

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