Comparison of three filter paper<b>-</b>based devices for safety and stability of viral sample collection in poultry

Wannaratana, Suwarak; Thontiravong, Aunyaratana; Pakpinyo, Somsak

doi:10.1080/03079457.2020.1837343

Cited by 5 publications

(9 citation statements)

References 34 publications

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“…However, 903 Protein Saver cards have an untreated matrix which does not guarantee virus inactivation. In fact, a recent study showed that purified Newcastle disease virus remained infectious in DBS after 24 h, in contrast to FTA cards 31 . Further studies analysing viral stability in patient samples would be necessary to check 903 Protein Saver cards safety levels for short and long-term storage of blood samples.…”

Section: Discussionmentioning

confidence: 98%

903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA

Arca-Lafuente

Casanueva-Benítez

Crespo-Bermejo

et al. 2022

Sci Rep

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Hepatitis C virus (HCV) infection remains a global health problem, detected only in the early stages by molecular tests. Molecular tests detect HCV RNA, which is very prone to degradation by ribonucleases, reason why blood samples must be transported and stored at − 20 °C, or even − 70 °C for long-term storage. Flinders Technology Associates (FTA) cards are a useful sampling collecting device for dry blood spot (DBS) storage, especially for low and middle-income countries (LMIC). In this study, we analyzed viral HCV RNA integrity for long-term storage at room temperature compared to − 20 °C using two different types of cards for DBS: FTA Classic and 903 Protein Saver cards. For this purpose, DBS were prepared on these cards using blood or plasma samples from HCV infected patients, and samples were analysed by conventional RT-PCR. Our results showed that 903 Protein Saver cards are the best and cheapest alternative for DBS storage at room temperature. In these conditions, we found that HCV RNA integrity lasted for up to 9 months.

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Section: Discussionmentioning

confidence: 98%

903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA

Arca-Lafuente

Casanueva-Benítez

Crespo-Bermejo

et al. 2022

Sci Rep

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“…The instability of RNA molecules is well known to impede the detection and characterization of viral RNA pathogens in the field. Several research groups, such as Cardona-Ospina et al [15], Hashimoto et al [16], Liang et al [17], and Wannaratana et al [18], have reported the benefits of using FTA cards to ensure reliable sample storage for identifying important viral diseases in animals. However, optimal handling, processing, and biosafety measures are not well established [15].…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of RNA viral nucleic acid-based detection of avian influenza virus, Newcastle disease virus, and African horse sickness virus on flinders technology associates card using conventional reverse-transcription polymerase chain reaction

et al. 2022

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Background and Aim: The flinders technology associates (FTA) card is a cotton-based cellulose membrane impregnated with a chaotropic agent that inactivates infectious microorganisms, lyses cellular material, and fixes DNA and/or RNA within the fiber matrix. However, little is known about the effectiveness of these cards for detecting RNA viruses in animals. This study aimed to evaluate the sensitivity of RNA virus detection using conventional reverse-transcription polymerase chain reaction (RT-PCR) on FTA cards. Materials and Methods: A highly virulent Newcastle disease virus (NDV) and an avian influenza virus (AIV) with low pathogenicity were propagated using chicken embryonic eggs. Three days after inoculation, the allantoic fluid was harvested, stored at –80°C, and the stock virus was tested for virus titration. African horse sickness virus (AHSV) was obtained from a live attenuated vaccine that was dissolved and stored at –80°C. For sample preparation, each stock virus was 10-fold serially diluted and each dilution was inoculated onto an FTA card, followed by drying in a Class II safety cabinet. Both the stock virus and infected FTA card were genomically isolated using an extraction kit, FTA purification kit, and extraction kit with Tris-EDTA (TE) buffer. The target genome was then detected by one-step RT-PCR for NDV and AIV, and two-step RT-PCR for African horse sickness, including gel electrophoresis for the detection of specific nucleic acids. Results: The detection limit of stock AIV was compared on FTA cards, using the FTA purification kit, and with TE buffer with an extraction kit. The corresponding results were 1.47, 1.17, and 2.18 log10 EID50, respectively, while for NDV the results were 4.13, 4.83, and 4.84 log10 ELD50. Finally, detection limit of stock AHSV and AHSV on the FTA card extracted using TE buffer with an extraction kit were 4.30 and 4.01 log10 plaque-forming units, respectively. Conclusion: This study demonstrated that the detection limit or sensitivity of all tested RNA viruses on FTA cards did not differ when compared with those of the stock virus and in both methods for RNA isolation on FTA cards. These cards are suitable for collecting and transporting samples infected with RNA viruses, particularly AIV, NDV, and AHSV. Flinders technology associates cards also provide hazard-free samples, a reliable source of RNA for molecular characterization, and sufficient quantity for diagnostic applications based on nucleic acid-based detection.

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“…Different DNA viruses usually demonstrated good storage stability in dried samples, for example, avian Marek’s disease virus (Cortes et al 2009 ), duck hepatitis B virus (Wang et al 2002 ) and fowl adenovirus I (Moscoso et al 2007 ), bovine herpesvirus-1 (Sarangi et al 2018 ) and bovine leucosis virus (Saushkin et al 2019 ; El Daous et al 2020 ), African swine fever virus (Michaud et al 2007 ; Uttenthal et al 2013 ; Randriamparany et al 2014 ). Common filter papers are cheap and could be as good as more expensive commercial FTA cards for virus storage, like it was shown for African swine fever virus (Uttenthal et al 2013 ; Randriamparany et al 2014 ), infectious bursal disease virus (Moscoso et al 2006 ; Maw et al 2006 ) and Marek’s disease virus (Cortes et al 2009 ; Wannaratana et al 2021 ). In another investigation, FTA cards and Whatmann 3MM filter paper (cheaper option) showed similar results for DBS long-term storage (up to 9 months) at ambient and tropical temperature (22, 32 and 37 oC) to detect RNA and DNA containing viruses (Michaud et al 2007 ).…”

Section: Dried Veterinary Samples Storage Stabilitymentioning

confidence: 99%

“…In case of PCR, FTA cards impregnated with detergents and other special substances are the most frequently used cards (Table 1 ). Moreover, they can provide virus inactivation, and are the safest to use among other Whatman filter papers (Wannaratana et al 2021 ). Wannaratana et al ( 2021 ) found that three Whatman cellulose carriers, namely FTA card, 903 card and qualitative filter paper grade 2 can preserve Marek’s disease virus DNA for at least 30 days post spot but only on FTA cards no viable Newcastle disease virus was detected at all post spotted timepoints.…”

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

“…Moreover, they can provide virus inactivation, and are the safest to use among other Whatman filter papers (Wannaratana et al 2021 ). Wannaratana et al ( 2021 ) found that three Whatman cellulose carriers, namely FTA card, 903 card and qualitative filter paper grade 2 can preserve Marek’s disease virus DNA for at least 30 days post spot but only on FTA cards no viable Newcastle disease virus was detected at all post spotted timepoints. For specific purposes, a common laboratory filter paper or stationary paper can be considered as affordable cost-efficient absorbing material in comparison with more expensive commercially available special sampling cards.…”

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

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Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

2022

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Dried Blood Spots (DBS) technology has become a valuable tool in medical studies, however, in veterinary and biological research DBS technology applications are still limited. Up-to-date no review has comprehensively integrated all the evidence existing across the fields, technologies and animal species. In this paper we summarize the current applications of DBS technology in the mentioned areas, and provide a scope of different types of dried sample carriers (cellulose and non-cellulose), sampling devices, applicable methods for analyte extraction and detection. Mammals, birds, insects and other species are represented as the study objects. Besides the blood, the review considers a variety of specimens, such as milk, saliva, tissue samples and others. The main applications of dried samples highlighted in the review include epidemiological surveys and monitoring for infections agents or specific antibodies for disease/vaccination control in households and wildlife. Besides the genetic investigations, the paper describes detection of environmental contaminants, pregnancy diagnosis and many other useful applications of animal dried samples. The paper also analyses dried sample stability and storage conditions for antibodies, viruses and other substances. Finally, recent developments and future research for DBS technology in veterinary medicine and biological sciences are discussed.

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Comparison of three filter paper-based devices for safety and stability of viral sample collection in poultry

Cited by 5 publications

References 34 publications

903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA

903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA

Sensitivity of RNA viral nucleic acid-based detection of avian influenza virus, Newcastle disease virus, and African horse sickness virus on flinders technology associates card using conventional reverse-transcription polymerase chain reaction

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

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