Comparison of three chromogenic media and enrichment broth media for the detection of methicillin-resistant Staphylococcus aureus from mucocutaneous screening specimens

Nonhoff, Claire; Denis, Olivier; Brenner, Anddon J.; Buidin, P.; Legros, Nicole; Thiroux, C.; Dramaix, M.; Struelens, Marc

doi:10.1007/s10096-008-0637-9

Cited by 48 publications

(34 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite commercial efforts to develop new media with high sensitivity, the present study, as well as others (MalhotraKumar et al, 2010;Nonhoff et al, 2009), showed a substantial increase in sensitivity following enrichment compared with direct cultures. Therefore, broth enrichment remains necessary for reliable MRSA detection.…”

Section: Resultscontrasting

confidence: 64%

“…The colours of the colonies ranged from light blue to the specific denimblue coloration considered typical for Brilliance MRSA Agar. In combination with broth enrichment, the negative predictive value of Brilliance MRSA Agar was comparable to that of MRSA ID medium Several studies have recently evaluated various novel chromogenic selective media for the detection of MRSA from clinical samples (Malhotra-Kumar et al, 2010;Nonhoff et al, 2009;Peterson et al, 2010). Comparison of the results of these studies is not easy because of differences in the study designs: swab type, body site sampled, direct inoculation versus prior homogenization in saline or broth, incubation time and the use of broth enrichment.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Clinical evaluation of Oxoid Brilliance MRSA Agar in comparison with bioMérieux MRSA ID medium for detection of livestock-associated meticillin-resistant Staphylococcus aureus

Verkade

Ferket

Kluytmans

2011

Journal of Medical Microbiology

View full text Add to dashboard Cite

Oxoid Brilliance MRSA Agar and bioMé rieux MRSA ID medium were evaluated for their ability to identify meticillin-resistant Staphylococcus aureus (MRSA) in clinical samples. Nasal and throat samples (n5629) were taken from veterinarians and their household members. The sensitivities of Brilliance MRSA Agar and MRSA ID medium after 20 h of incubation were 63.6 and 64.5 %, and the specificities were 94.1 and 99.4 %, respectively. After an enrichment step, the sensitivities increased to 96.3 and 97.2 %, but the specificities decreased to 88.7 and 98.5 %, respectively. Brilliance MRSA Agar and MRSA ID medium are both sensitive methods for the screening of MRSA in combination with broth enrichment, but positive results require confirmation.

show abstract

Section: Resultscontrasting

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Clinical evaluation of Oxoid Brilliance MRSA Agar in comparison with bioMérieux MRSA ID medium for detection of livestock-associated meticillin-resistant Staphylococcus aureus

Verkade

Ferket

Kluytmans

2011

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…After 48 h of incubation, 170 out of 243 specimens with mauve colonies on CMRSAII did not grow MRSA on the reference plate, but all of these mauve colonies could be easily proven to be MRSA by using a confirmatory test, such as coagulase or cefoxitin disk diffusion testing. When a confirmatory test was included, the specificity of CMRSAII was 100% and was comparable to or better than those described for other chromogenic media (9,10,12,19,22,23,26). In conclusion, CMRSAII is a reliable screening medium for multiple specimen types.…”

Section: Discussionsupporting

confidence: 59%

“…Since there is a lack of a gold standard for MRSA screening, most authors compared the recovery rate of a specific medium with the combined result of all media or methods that have been used (5,6,9,10,12,13,15,19,20,(21)(22)(23)(24)(26)(27)(28)(29)(30). This prevents the misclassification of true-positive results, i.e., detection of MRSA classified as false positive if the comparison method failed to detect MRSA.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a New Selective Medium, BD BBL CHROMagar MRSA II, for Detection of Methicillin-Resistant Staphylococcus aureus in Different Specimens

et al. 2010

View full text Add to dashboard Cite

The sensitivity of screening for methicillin-resistant Staphylococcus aureus (MRSA) can be improved by adding other specimen sites to nares. We describe an evaluation of a new selective medium, BBL CHROMagar MRSA II (CMRSAII), for its ability to detect MRSA from different specimen types. CMRSAII is a chromogenic medium which incorporates cefoxitin for the detection of MRSA. A study was performed at four clinical laboratories with the following specimens: 1,446 respiratory, 694 stool, 1,275 skin, and 948 wound specimens and 688 blood culture bottles containing Gram-positive cocci. The recovery of MRSA on traditional culture media was compared to results with CMRSAII. S. aureus was tested by cefoxitin disk diffusion. CMRSAII was interpreted as positive for MRSA at 24 h (range, 18 to 28 h) based solely on the visualization of mauve-colored colonies and at 48 h (range, 36 to 52 h) based on detection of mauve colonies with subsequent confirmation as S. aureus (by coagulase or latex agglutination testing). MRSA was recovered more frequently on CMRSAII (89.8% at 24 h and 95.6% at 48 h) than on traditional culture plates (83.1% at 24 h and 79.8% at 48 h) for all specimen types combined (P < 0.001). The percent sensitivities of CMRSAII at 24-and 48-h reads, respectively, were 85.5 and 92.4% for respiratory specimens, 87.9% and 98.3% for stool specimens, 88.4% and 96.1% for skin specimens, 92.1% and 94.6% for wound specimens, and 100% and 100% for positive blood cultures. The specificity was 99.8% for respiratory specimens and 100% for all others. In conclusion, CMRSAII is a reliable screening medium for multiple specimen types.

show abstract

“…Several chromogenic agars have been specifically developed for MRSA screening. These media show superior sensitivity and specificity compared to those of conventional selective plates (8)(9)(10). The purpose of this study was to compare the performance of the new bioMérieux chromID MRSA SMART (SMART) agar with bioMérieux chromID MRSA first generation (chromID) and Oxoid Brilliance MRSA version 2 agar (OX2) with and without enrichment broth (EB).…”

mentioning

confidence: 99%

Prospective Two-Center Comparison of Three Chromogenic Agars for Methicillin-Resistant Staphylococcus aureus Screening in Hospitalized Patients

et al. 2015

View full text Add to dashboard Cite

bThree chromogenic media, chromID MRSA SMART (SMART), chromID MRSA first generation (chromID), and Brilliance MRSA (OX2), were evaluated for methicillin-resistant Staphylococcus aureus (MRSA) screening using 1,220 samples. The sensitivity at 24 h was significantly better with the SMART agar (66.4%) than that with chromID agar (50.5%). Enrichment and incubation until 48 h are still needed for an optimal yield. M ethicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections (1, 2). Asymptomatic carriers in the nose, throat, or on the skin represent the major reservoirs for MRSA transmission in hospitals (3, 4). Rapid identification of MRSA colonization is of utmost importance to identify carriers in order to implement infection control procedures and reduce patient-to-patient transmission (5-7). Several chromogenic agars have been specifically developed for MRSA screening. These media show superior sensitivity and specificity compared to those of conventional selective plates (8-10). The purpose of this study was to compare the performance of the new bioMérieux chromID MRSA SMART (SMART) agar with bioMérieux chromID MRSA first generation (chromID) and Oxoid Brilliance MRSA version 2 agar (OX2) with and without enrichment broth (EB).This prospective study was conducted between January and May 2014 at Hôpital Erasme, Brussels, Belgium, and Amphia Hospital, Breda, The Netherlands. Screening samples were collected from patients admitted to both hospitals using ESwab transport medium (Copan Diagnostics). The swabs were inoculated into EB supplemented with 6.5% NaCl (bioMérieux) and directly on the SMART, chromID, and OX2 agars, and a Colombia agar plate with 5% sheep blood (SBA) (growth control) at 35 to 37°C. EB was subcultured after 24 h onto secondary selective plates. Primary plates were examined at 24 h and 48 h, and secondary plates were examined after 24 h. The assessments included comparative amounts of growth of presumptive MRSA/sample and the amount of background flora able to grow on the three media. All suspected MRSA colonies were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and methicillin susceptibility was determined by the cefoxitin disk method, as recommended by EUCAST (11).Each new MRSA isolate per patient and discrepant results between the three media were tested for the presence of the mecA gene by in-house PCR (Erasme) (12) or GeneXpert (Xpert SA nasal G3 kit version 4; Cepheid) (Amphia). Discrepant results (typical MRSA colonies and mecA negative) were further analyzed for the presence of the mecC gene, and the MICs to cefoxitin and oxacillin were determined (11, 13). The presence of MRSA recovered from at least one of the media (primary chromogenic agar or after enrichment) was considered the gold standard. True-positive results were defined as MRSA isolates showing characteristic colonies (color and morphology) confirmed by PCR, false-positive results as isolates with typical colonies not confirmed by PC...

show abstract

Comparison of three chromogenic media and enrichment broth media for the detection of methicillin-resistant Staphylococcus aureus from mucocutaneous screening specimens

Cited by 48 publications

References 28 publications

Clinical evaluation of Oxoid Brilliance MRSA Agar in comparison with bioMérieux MRSA ID medium for detection of livestock-associated meticillin-resistant Staphylococcus aureus

Clinical evaluation of Oxoid Brilliance MRSA Agar in comparison with bioMérieux MRSA ID medium for detection of livestock-associated meticillin-resistant Staphylococcus aureus

Evaluation of a New Selective Medium, BD BBL CHROMagar MRSA II, for Detection of Methicillin-Resistant Staphylococcus aureus in Different Specimens

Prospective Two-Center Comparison of Three Chromogenic Agars for Methicillin-Resistant Staphylococcus aureus Screening in Hospitalized Patients

Contact Info

Product

Resources

About