Comparison of Thiopental, Urethane, and Pentobarbital in the Study of Experimental Cardiology in Rats In Vivo

Żorniak, Michał; Mitręga, Katarzyna; Białka, Szymon; Porc, Maurycy; Krzemiński, Tadeusz F.

doi:10.1097/fjc.0b013e3181dd502c

Cited by 30 publications

(24 citation statements)

References 32 publications

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“…According the results of this study, pentobarbital is the most suitable anesthetic offering stable hemodynamic values during arrhythmia studies. These hemodynamic values were similar to physiological values in awake rats; the long arrhythmia duration during reperfusion and approximately 50% mortality index are crucial parameters for evaluating antiarrhythmic drugs [34]. …”

Section: Barbiturates (Thiopental and Pentobarbital) Etomidate Asupporting

confidence: 57%

The Effect of Intravenous Anesthetics on Ischemia-Reperfusion Injury

Eroğlu

2014

BioMed Research International

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The effects of intravenous anesthetics on ischemia-reperfusion injury (IRI) have been investigated in both animals and clinical studies. The protective effects and the dosages of the intravenous anesthetics on IRI were discussed in this paper. The prevention of the tissue injury after the IRI was demonstrated with intravenous anesthetics in some studies. In the future, the studies should be focused on the dosage of the anesthetics related to diminishing the tissue injuries. Further studies might be required in order to investigate the effects of the anesthetics on molecular levels.

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Section: Barbiturates (Thiopental and Pentobarbital) Etomidate Asupporting

confidence: 57%

The Effect of Intravenous Anesthetics on Ischemia-Reperfusion Injury

Eroğlu

2014

BioMed Research International

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“…However, there are only a few studies addressing the influence of anesthetics on I/R-induced arrhythmias (7)(8)(9)(10), whilst there has been no comparison between these two commonly used anesthetics (thiopental and K+X) in an in vivo I/R arrhythmia model. With this in mind, the aim of the present study was to compare the duration and severity of ventricular arrhythmias induced by coronary artery occlusion and reperfusion, as well as the hemodynamic parameters between thiopental-and K+X-anesthetized rats.…”

Section: Introductionmentioning

confidence: 99%

“…The use of different types of anesthesia may be the reason for certain discrepancies between the results of ischemia/reperfusion (I/R)-induced arrhythmia studies in the literature. The anesthetic agents used in this model were reported to influence the occurrence of I/Rinduced arrhythmias (7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Comparison of thiopental and ketamine+xylazine anesthesia inischemia/reperfusion-induced arrhythmias in rats

Gonca¹

2015

Turk J Med Sci

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Background/aim: To investigate the influence of thiopental (85 mg/kg, intraperitoneally (ip)), and ketamine+xylazine (ketamine 75 mg/kg and xylazine 8 mg/kg, ip) anesthesia on the incidence and duration of ischemia/reperfusion-induced arrhythmias.Materials and methods: Myocardial ischemia was induced by a 6-min ligation of the left anterior descending coronary artery, followed by a 6-min reperfusion. Measurements were taken of the incidence and duration of ventricular arrhythmias, the mean arterial blood pressure and heart rate, and the pressure rate-product (as an index of myocardial oxygen consumption). Results:The arrhythmia score and the incidence of ventricular fibrillation and tachycardia were significantly decreased in the ketamine+xylazine-anesthetized rats compared with the thiopental-anesthetized group (arrhythmia score: 2.0 ± 2.1 versus 3.7 ± 1.2, P < 0.05). The heart rate was significantly lower in the ketamine+xylazine group during the entire experiment, whilst the pressure-rate product was also significantly lower in the ketamine+xylazine group at different time points of the ischemia and reperfusion periods. Conclusion:Ketamine+xylazine anesthesia has a strong antiarrhythmic effect and an apparent depressive action on the heart rate and the myocardial oxygen consumption index. Therefore, ketamine+xylazine anesthesia is not appropriate for the evaluation of possible antiarrhythmic agents. Thiopental anesthesia is preferable to ketamine+xylazine anesthesia in the in vivo ischemia-reperfusion arrhythmia model.

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“…24 At the end of the experimental study the body weights of rats were estimated, and also SBP estimation was done by the Power Lab (4/35) data acquisition system (Australia) after anesthetizing rats with an intraperitoneal injection of pentobarbital (60 mg/kg, Sigma, St. Louis, USA). 28,29 Carotid cannulation was done in which one end Pharmaceutical Sciences, 2020, 26(1), 13-24 | 15 of the cannula was into the carotid artery however the other was connected to a three-way stopcock. The pressure transducer was connected to this three-way stopcock together with a heparinized saline-filled syringe that helped the application and maintenance of positive pressure at the baseline level.…”

Section: Animal Sacrifaction and Blood Pressure Monitoringmentioning

confidence: 99%

Alagebrium Mitigates Metabolic Insults in High Carbohydrate and High Fat Diet Fed Wistar Rats

et al. 2020

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Background: Metabolic syndrome (MS) is characterized by sustained hyperglycemia that triggers advanced glycation end products (AGEs) generation. Alagebrium (ALA) is an advanced glycation end products (AGEs) cross-links breaker.Methods: 32 Wistar rats were divided into normal control (NC) group (8 rats) and MS groups (24 rats) received a high carbohydrate high fat diet (HCFD) for 10 weeks. Rats with established MS were equally divided into 3 subgroups remained on HCFD for further 6 weeks: MS control (MSC), ALA treated received 10 mg/kg/day ALA orally and metformin treated (MF) (a reference drug) received 50 mg/kg/day MF orally. The studied parameters were systolic blood pressure (SBP), body and liver weights (BW, LW), LW/BW% ratio, fasting blood glucose (FBG), serum insulin, lipid profile, liver enzymes, serum AGEs, hepatic Interleukin-17 (IL-17), adipokines, pAkt/Akt ratio, and liver histopathology. Results: HCFD elevated SBP, BW, LW and LW/BW% ratio, FBG, serum insulin, and AGEs. It also deteriorated lipid profile and liver enzymes, induced inflammation, insulin resistance and histopathological derangements. ALA ameliorated the elevated SBP, FBG, lipid profile, liver enzymes, mitigated insulin resistance, hepatic IL-17, serum AGEs, modulated adipokines levels and improved liver histopathology. However, MF had better effects than ALA in all studied parameters except AGEs. Conclusion: ALA is protective against dietary-induced MS via ameliorating the inflammatory process and serum AGEs that implicated in MS pathogenesis, which makes it a promising new tool in MS treatment.

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Comparison of Thiopental, Urethane, and Pentobarbital in the Study of Experimental Cardiology in Rats In Vivo

Cited by 30 publications

References 32 publications

The Effect of Intravenous Anesthetics on Ischemia-Reperfusion Injury

The Effect of Intravenous Anesthetics on Ischemia-Reperfusion Injury

Comparison of thiopental and ketamine+xylazine anesthesia inischemia/reperfusion-induced arrhythmias in rats

Alagebrium Mitigates Metabolic Insults in High Carbohydrate and High Fat Diet Fed Wistar Rats

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