Comparison of ThinPrep and conventional smears in salivary gland fine-needle aspiration biopsies

Parfitt, Jeremy; McLachlin, C. Meg; Mr, Weir

doi:10.1002/cncr.22575

Cited by 43 publications

(60 citation statements)

References 11 publications

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“…While there is a large body of literature discussing findings in nongynecological specimens by TP, the literature on salivary gland FNA biopsies using this technique is extremely limited. As noted by previous reports [1,2,3], LBC introduces new artifacts that alter the cellular, architectural and extracellular matrix appearance (table 1). The authors emphasized the need for becoming familiar with those artifacts prior to full implementation of this new technology for patient care.…”

Section: Introductionmentioning

confidence: 92%

“…This methodology has several advantages over conventional smears (CS), including the following: (1) more standardized processing, especially with samples received from outside clinics or shipped from a distance to centralized laboratories; (2) decreased screening area; (3) decreased obscuring material such as blood, inflammation and mucus; (4) lack of air-drying artifacts; (5) a more monolayer cellular surface that is easier to screen; (6) consistently well-preserved cells, and (7) the potential for automated screening in LBC specimens [1,2,3,4]. Because of the many advantages of LBC, the technique has been increasingly adopted by many laboratories for nongynecological specimens, particularly exfoliative samples.…”

Section: Introductionmentioning

confidence: 99%

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The Utility of Liquid-Based Cytology in Salivary Gland Fine-Needle Aspirates: Experience of an Academic Institution

Rarick

Wasman²,

Michael³

2014

Acta Cytologica

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Objective: To review the morphological features of salivary gland fine-needle aspirates (FNA) on ThinPrep® (TP) preparations. Emphasis is placed on the commonalities and specific differences between TP and conventional smear (CS) preparations. Study Design: The cytology and surgical pathology archives were searched for ‘salivary' or ‘parotid' at our institution from 2003 to 2013 for cases processed by TP and with a range of specified diagnoses and surgical follow-up. These cases were reviewed by the authors. A review of the known literature was also performed. Results: Morphological features and artifacts were noted for the most common salivary gland lesions. General features noted in the literature were identified, along with novel features identified on our review of cases. Emphasis was placed on cellular alterations, extracellular differences and architectural changes. Conclusions: The multitude of both benign and malignant lesions coupled with the unique artifacts encountered on TP makes salivary gland FNAs difficult for most practicing pathologists and cytopathologists. For this reason, many laboratories have been reluctant to implement TP preparations for these lesions. With knowledge of the specific artifacts and differences on TP compared to CS, TP can be used with confidence without a compromise in diagnostic accuracy for adequately cellular samples.

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Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The Utility of Liquid-Based Cytology in Salivary Gland Fine-Needle Aspirates: Experience of an Academic Institution

Rarick

Wasman²,

Michael³

2014

Acta Cytologica

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“…LBC is increasingly applied to this scenario. Parfitt et al [22] evaluated salivary gland lesions with conventional smears and TP-prepared specimens with the following findings:…”

Section: Fine-needle Aspirationmentioning

confidence: 99%

Overview of Nongynecological Samples Prepared with Liquid-Based Cytology Medium

Ren

Solomides²,

Draganova‐Tacheva³

et al. 2014

Acta Cytologica

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Objective: Liquid-based cytology of nongynecological specimens is commonly used in cytology laboratories throughout the world and various processing methods, such as ThinPrep and SurePath, have been reported. The cytological features and performance of liquid-based cytology for various cytology specimens, including body cavity fluids, urine, brushing specimens and fine-needle aspiration of various lesions, were reviewed and compared with the experience of our laboratory and the literature published in PubMed. Study Design: The parameters for the evaluation of liquid-based cytology and conventional smears were described in the various types of specimens. Criteria for the interpretation of nongynecological liquid-based cytology were highlighted to show differences in cell morphology, background and artifacts. Results: The interpretation requires familiarity with the appearance of liquid-based cytology in the various types of preparations to avoid misdiagnosis. Conclusions: Cell blocks can be prepared with specimens preserved in a liquid-based cytology medium and immunocytochemical stains and molecular testing can be successfully performed. These are important adjuncts in order to reach a definitive diagnosis.

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“…7,8 Settings or specific diagnoses in which background material is critical to establishing the diagnosis (some sarcomas, salivary gland tumors, mucinous tumors) may be more difficult to accurately diagnose with TP. 2,9,10 The utility of TP in lung cytology has been addressed in few studies, and in these studies only a small number of cases were small cell carcinoma of lung (SCCL). [11][12][13] Thus, the utility and limitations of TP in the cytologic diagnosis of SCCL is still evolving.…”

mentioning

confidence: 99%

Analysis of ThinPrep cytology in establishing the diagnosis of small cell carcinoma of lung

Kim

Owens

2009

Cancer Cytopathology

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BACKGROUND:ThinPrep liquid‐based cytology (TP) preparations are being used increasingly in nongynecologic specimens. To the authors' knowledge, few studies to date have evaluated TP cytology in the setting of small cell carcinoma of the lung (SCCL). Accurately differentiating SCCL from other lung tumors has important clinical and therapeutic implications. Herein, the authors evaluated the diagnostic utility and cytomorphology of TP in the setting of SCCL.METHODS:All cases of SCCL with prior or concurrent TP cytologic specimens were identified via computer search. The cytodiagnoses were tabulated. When available, cytologic material was reviewed. Performance parameters of the various cytologic modalities processed with TP were calculated.RESULTS:In 121 patients with SCCL, 261 TP specimens were identified. The cytodiagnoses were: SCCL (119 specimens), suspicious for SCCL (45 specimens), atypical cells‐not otherwise specified (22 specimens), negative/nondiagnostic (63 specimens), and nonsmall cell carcinoma (4 specimens). During the same period of time, 3 cases of false‐positive diagnoses of SCCL were identified. The positive predictive value for a cytodiagnosis of SCCL on TP was 97.5%, and the sensitivity was 62.8%. Bronchial brush was the most sensitive cytologic modality (78.3%). Immunostaining was found to be contributory to the diagnosis in 10 of the 11 cases in which it was attempted.CONCLUSIONS:TP is a sound alternative to conventional preparations for the cytodiagnosis of SCCL. Cytomorphology of SCCL is altered in TP with less molding, less cell fragility, more discohesion, and tumor cell shrinkage artifact. Immunohistochemical staining of cellblocks is a useful adjunct in challenging cases. A positive cytodiagnosis of SCCL on TP can be used to initiate definitive therapy when biopsy is not possible. Cancer (Cancer Cytopathol) 2009. © 2009 American Cancer Society.

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Comparison of ThinPrep and conventional smears in salivary gland fine-needle aspiration biopsies

Cited by 43 publications

References 11 publications

The Utility of Liquid-Based Cytology in Salivary Gland Fine-Needle Aspirates: Experience of an Academic Institution

The Utility of Liquid-Based Cytology in Salivary Gland Fine-Needle Aspirates: Experience of an Academic Institution

Overview of Nongynecological Samples Prepared with Liquid-Based Cytology Medium

Analysis of ThinPrep cytology in establishing the diagnosis of small cell carcinoma of lung

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