Overview of Nongynecological Samples Prepared with Liquid-Based Cytology Medium

Ren, Shuyue; Solomides, Charalambos; Draganova‐Tacheva, Rossitza; Bibbo, Marluce

doi:10.1159/000363123

Cited by 23 publications

(22 citation statements)

References 38 publications

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“…[6] Hence, interpreting pathologists should be cautious to avoid misinterpretations while reporting FNA prepared using LBC if that is the only methodology employed.…”

Section: Discussionmentioning

confidence: 99%

Utility of manual liquid-based cytology and conventional smears in the evaluation of various fine-needle aspiration samples

Arul

2016

J Cytol

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Background:Liquid-based cytology (LBC) preparation is a way to improve and refine the fine-needle aspiration (FNA) samples. There are a few studies comparing LBC with conventional smear (CS).Aim:The present study was undertaken to evaluate the utility of manual LBC (MLBC) and CS preparations in various FNA samples.Materials and Methods:In this cross-sectional study, a total of 100 FNA samples from various anatomical sites were evaluated using MLBC and CS preparations. Cellularity, blood, informative background, monolayers, cell architecture, cytoplasmic, and nuclear preservation were compared with MLBC and CS preparations by Wilcoxon signed rank test. P < 0.05 is considered statistically significant.Results:MLBC preparations were superior to CS preparations in view of absence of blood and debris (P = 0.001), presence of monolayers (P < 0.001), and preservation of cytoplasmic (P = 0.001) and nuclear details (P = 0.001). However, no statistically significant differences were found between MLBC and CS preparations with regard to cellularity (P = 0.157), informative background (P = 0.083), and architecture (P = 0.739).Conclusion:MLBC preparations in FNAC are a safe, easy, and less time-consuming procedure, and it may have promising diagnostic value in the evaluation of FNA samples from various anatomical sites. However, the use of both MLBC and CS preparations is recommended to achieve optimal diagnostic yield.

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“…[6] Hence, interpreting pathologists should be cautious to avoid misinterpretations while reporting FNA prepared using LBC if that is the only methodology employed.…”

Section: Discussionmentioning

confidence: 99%

Utility of manual liquid-based cytology and conventional smears in the evaluation of various fine-needle aspiration samples

Arul

2016

J Cytol

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“…However, because of the chemical influences of the fixation medium and the physical forces of processing techniques, LBC tends to produce certain cytomorphological alterations and artefacts: smaller cell clusters and sheets and breakage of papillae; more dyscohesive cells; attenuated chromatin details with prominent nucleoli and smaller cell size; intranuclear inclusion is difficult to visualize; altered background matrix in both quantity and quality; aggregation of lymphocytes and markedly decreased number of extracellular particles; and small mononuclear cells, red blood cells, and myoepithelial cells [15].…”

Section: Discussionmentioning

confidence: 99%

“…Pervez et al concluded that the diagnosis of fibroadenoma seems to be most problematic on LBC preparations [19]. Some studies showed a low diagnostic rate compared to CS and false-positive diagnoses while over-classifying fibroadenomas as atypical or suspicious [10][11][12][13][14][15][16][17][18][19]20].…”

Section: Discussionmentioning

confidence: 99%

Validity of UPREP liquid based cytology in FNAC examination for palpable lesions type of article – original article

J¹,

Aswathy²

2019

Trop J Pathol Microbiol

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Background: Liquid-based cytology (LBC) is a technique that enables cells to be suspended in a monolayer, through which better morphological assessment is possible. There are very few studies done to evaluate the outcomes between conventional and UPREP LBC method. A successful validation of UPREP LBC will go a long way in providing both affordable and accurate management of cancers in developing countries. Methods: This study was carried out as a comparative study among 110 all adults who were advised to undergo Fine Needle Aspiration Cytology (FNAC) examination for palpable lesions in thyroid, breast and lymph node in our tertiary care hospital. Conventional FNAC was taken for 55 participants and for the UPREP LBC group (55 participants), the same procedure was repeated and instead of air, preservative solution was drawn into the syringe. Various cytological parameters including cellularity, background, monolayer, cellular morphologic change, nuclear changes and inflammatory infiltrate were studied. Results: Among the individual lesions involving breast, thyroid and lymph node, breast carcinoma was highest in both the groups (25.4%) followed by fibroadenoma of the breast (20%) and Hashimoto's thyroiditis (18.2%). Statistically significant differences were observed between the conventional and UPREP groups. Informative background was increasingly better in the conventional group compared to the UPREP group (p<0.001) while background debris was absent in most of the patients in UPREP group (p<0.001). Details pertaining to nucleus and cytoplasm were better perceived in the UPREP group compared to the conventional group (p<0.001). Conclusion: Manual liquid based cytology like UPREP LBC is an accurate, less expensive alternative procedure to automated LBC with the advantage of providing monolayer, absence of obscuring blood or debris, better nuclear and cytoplasmic morphology.

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“…ThinPrep TM might have induced artifacts and cellular morphologic alterations, which influenced diagnostic accuracy. The interpretation requires familiarity with cytological appearance to avoid misdiagnosis [15]. For thyroid lesions the colloid is seen as droplets rather than a diffuse pattern [7].…”

Section: Diagnostic Category For Direct Smear Materialsmentioning

confidence: 99%

Evaluation on Adequacy and Diagnostic Comparability of Discarded Residual Materials of Fine-needle Aspirations Processed using ThinprepTM for Lesions on Four Different Organs

Zawawi¹,

Othman²

2015

J Cytol Histol

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Objective:The study was to evaluate the adequacy and diagnostic accuracy of discarded residual materials of fine-needle aspirations (FNA) processed using ThinPrep TM on four different organs; breast, thyroid, lymph nodes and salivary glands.Study Designs: Aspirated materials of these lesions were directly smeared on glass slides as routinely done. The needles used were then vigorously rinsed by drawing in the ThinPrep TM medium in order to obtain the needleresidual materials. The direct smears [the gold standard] were reviewed by pathologists on rotation and each was given a diagnosis as per routine testing. The needle-residual material smears [test method] were reviewed separately by one pathologist who was blinded to the diagnoses made.Results: A total of 118 cases comprising 47(39.8%) breasts, 42(35.6%) thyroid, 25(21.2%) lymph node and 4(3.4%) salivary glands lesions were included. The overall diagnostic accuracy of needle-residual materials was comparable to the direct smears materials with overall kappa score of 0.653. Lymph nodes, breast and salivary glands diagnostic accuracy show good agreement (kappa: 0.769, 0.636 and 0.617 respectively). Moderate agreement was seen in thyroid (kappa: 0.569). Conclusion:Residual materials left in needles have sufficient materials for cytological assessment for future molecular studies if needed.

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Overview of Nongynecological Samples Prepared with Liquid-Based Cytology Medium

Cited by 23 publications

References 38 publications

Utility of manual liquid-based cytology and conventional smears in the evaluation of various fine-needle aspiration samples

Utility of manual liquid-based cytology and conventional smears in the evaluation of various fine-needle aspiration samples

Validity of UPREP liquid based cytology in FNAC examination for palpable lesions type of article – original article

Evaluation on Adequacy and Diagnostic Comparability of Discarded Residual Materials of Fine-needle Aspirations Processed using ThinprepTM for Lesions on Four Different Organs

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