Comparison of the Vidas System and Two Recent Fully Automated Assays for Diagnosis and Follow-Up of Toxoplasmosis in Pregnant Women and Newborns

Murat, Jean-Benjamin; Dard, Céline; Hidalgo, Hélène Fricker; Dardé, Marie‐Laure; Brenier-Pinchart, Marie-Pierre; Pelloux, Hervé

doi:10.1128/cvi.00089-13

Cited by 41 publications

(32 citation statements)

References 32 publications

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“…However, adjusting the PPV in our study for epidemiological data is not possible, since prevalence and incidence data are not available for the various regions where the specimens were sent from. Laboratory testing for toxoplasmosis, including IgG, IgM, and IgG avidity, varies greatly by method and kit (23)(24)(25). Furthermore, the specificity of IgM has been found to vary from lot to lot even from the same manufacturer (14).…”

Section: Discussionmentioning

confidence: 99%

Significance of a Positive Toxoplasma Immunoglobulin M Test Result in the United States

Dhakal¹,

Gajurel

Pomarès

et al. 2015

J Clin Microbiol

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Section: Discussionmentioning

confidence: 99%

Significance of a Positive Toxoplasma Immunoglobulin M Test Result in the United States

Dhakal¹,

Gajurel

Pomarès

et al. 2015

J Clin Microbiol

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“…The direct detectionof T. gondii is difficult, therefore, most clinical laboratories use serological tests to detect antibodies against T. gondii, such as the latex agglutination test, enzyme-linked immunosorbent assay (ELISA), and indirect fluorescent antibody test (IFAT) (7,8). Toxoplasmosis among pregnant women is a major concern in both developed and developing countries.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence, Seroconversion, and Risk Factors for Toxoplasmosis among Pregnant Women in Taipei, Taiwan

Hung

Lee

et al. 2015

Jpn J Infect Dis

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SUMMARY: Herein, we determined the seroprevalence, seroconversion, and risk factors associated with Toxoplasma gondii (T. gondii) infection among pregnant women in Taipei, Taiwan. Pregnant women attending antenatal consultation in a Taipei medical center were invited, and 104 women completed a self-administered structured questionnaire. Venous blood samples were collected during the first and third trimester after consent was obtained. Serum IgG and IgM antibodies (Abs) as well as IgG avidity were analyzed using an enzyme-linked fluorescent assay. Of the samples collected in the first trimester, seven were seropositive for IgG Abs and one was seropositive for IgG ＋ IgM Abs with a borderline avidity index, resulting in an overall seroprevalence of 7.7z. No statistically significant association was found between toxoplasmosis and age, pregnancy history, or any risk factors. Seroconversion was not detected from paired sera between the first and third trimesters. Pregnant women with senior high school education level or those who claimed to knowing Toxoplasma exhibited a significantly higher seroprevalence than those with bachelor degree (P ＝ 0.05) or those who claimed not to have this knowledge (P ＝ 0.05). Therefore, failure to understand the importance of T. gondii infection and the prevention measures resulted in the development of toxoplasmosis among these women.

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“…In this study, ELFA detected more cases positive for IgM antibodies in G2 and more cases with IgG antibodies in G1. This higher positivity may be related to the fact that ELFA is an automated method; for some authors, automated methods are more sensitive and specific (Calderaro et al, 2008;Kasper et al, 2009;Maudry et al, 2009;Murat et al, 2013b;Petersen et al, 2005;Prusa et al, 2010;Rodrigues et al, 2009;Wilson et al, 1997). In this study, ELFA and ELISA had sensitivities of 100% for IgG, and 79.2% and 75.5% for IgM, respectively (Table 3).…”

Section: Discussionmentioning

confidence: 61%

“…The results of qPCR are understandable as the result of serology suggests an acute infection, which is characterized by periods of parasitemia (Cordeiro et al, 2010). Moreover, qPCR is considered by some authors as a more sensitive technique with low risks of contamination (Homan et al, 2000;Lin et al, 2000;Murat et al, 2013b).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil

Murata

Ferreira

Pereira-Chioccola

et al. 2017

Diagnostic Microbiology and Infectious Disease

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Toxoplasmosis during pregnancy can have severe consequences. The use of sensitive and specific serological and molecular methods is extremely important for the correct diagnosis of the disease. We compared the ELISA and ELFA serological methods, conventional PCR (cPCR), Nested PCR and quantitative PCR (qPCR) in the diagnosis of Toxoplasma gondii infection in pregnant women without clinical suspicion of toxoplasmosis (G1=94) and with clinical suspicion of toxoplasmosis (G2=53). The results were compared using the Kappa index, and the sensitivity, specificity, positive predictive value and negative predictive value were calculated. The results of the serological methods showed concordance between the ELISA and ELFA methods even though ELFA identified more positive cases than ELISA. Molecular methods were discrepant with cPCR using B22/23 primers having greater sensitivity and lower specificity compared to the other molecular methods.

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Comparison of the Vidas System and Two Recent Fully Automated Assays for Diagnosis and Follow-Up of Toxoplasmosis in Pregnant Women and Newborns

Cited by 41 publications

References 32 publications

Significance of a Positive Toxoplasma Immunoglobulin M Test Result in the United States

Significance of a Positive Toxoplasma Immunoglobulin M Test Result in the United States

Seroprevalence, Seroconversion, and Risk Factors for Toxoplasmosis among Pregnant Women in Taipei, Taiwan

Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil

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