Comparison of the toxicity of the dyes Sudan II and Sudan IV to catalase

Li, Tong; Hao, Minglu; Pan, Jie; Zong, Wansong; Liu, Rutao

doi:10.1002/jbt.21943

Cited by 8 publications

(2 citation statements)

References 49 publications

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“…Sudan dyes are synthetic industrial dyes that are widely used as a colorant in oil, wax, gasoline, shoes, and floor polishes (Teng & Zhou, 2017). Sudan dyes are carcinogenic, genotoxic, sensitive, and mutagenic (Li et al, 2017;Xu et al, 2010), and are toxic to human organs such as the liver and kidney. The International Agency for Research on Cancer (IARC) has analyzed and evaluated the carcinogenic effect of Sudan Red and classified it as a category 3 carcinogen (Zuckerman, 1995).…”

Section: Introductionmentioning

confidence: 99%

Rapid Determination of Sudan Dyes in chilli products using ultra high performance supercritical fluid chromatography-photodiode array detection

Nie

Xie

Wang

et al. 2021

CyTA - Journal of Food

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A novel, rapid, and reliable ultra-high performance supercritical fluid chromatography (UHPSFC) method was developed to correctly separate and quantitatively determine Sudan dyes in chilli products. The effect of different separation parameters on the retention characteristics of Sudan dyes was investigated. The results showed that diode array detection of Sudan I-II was accomplished at 480 nm, and that of Sudan III-IV at 500 nm. In the analysis of Sudan dyes, the whole analysis time was less than 10 minutes, the method had a limit of detection and limit of quantification of 0.25 μg/mL and 0.50 μg/mL, respectively. All calibration curves had good linearity (r 2 > 0.9989) within the tested concentration ranges. The method had intra-and inter-day precision of less than 3.5% and 4.1%, respectively, with a recovery between 96.58% and 104.50%, and a relative standard deviation of less than 4.4%. The method was successfully applied to analyze chilli product sample.Determinación rápida de colorantes de Sudán en productos de chile utilizando cromatografía de fluido supercrítico de ultra alto rendimiento-detección de matriz de fotodiodos RESUMEN El presente estudio se propuso desarrollar un método novedoso, rápido y fiable de cromatografía de fluidos supercríticos de alto rendimiento (UHPSFC) para separar correctamente y determinar cuantitativamente los colorantes Sudán en productos de chile. Para ello, se investigó el efecto de diferentes parámetros de separación en las características de retención de los colorantes Sudán. Los resultados permitieron constatar que la detección por matriz de diodos de Sudán I-II se logró a 480 nm, mientras que la de Sudán III-IV ocurrió a 500 nm. Asimismo, al analizar los colorantes de Sudán, la totalidad del tiempo de análisis fue inferior a 10 minutos, el método tuvo un límite de detección y un límite de cuantificación de 0.25 μg/mL y 0.50 μg/mL, respectivamente. Todas las curvas de calibración mostraron buena linealidad (r 2 > 0.9989) dentro de los rangos de concentración probados. Además, el método tuvo una precisión intra e interdiaria inferior a 3.5 y a 4.1%, respectivamente, registrando una recuperación de entre 96.58 y 104.50%, y una desviación estándar relativa inferior a 4.4%. En conclusión, el método se aplicó con éxito para analizar muestras de productos de chile.

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Section: Introductionmentioning

confidence: 99%

Rapid Determination of Sudan Dyes in chilli products using ultra high performance supercritical fluid chromatography-photodiode array detection

Nie

Xie

Wang

et al. 2021

CyTA - Journal of Food

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“…Catalase is one of the most important enzymes to protect from oxidative damage by excessive H 2 O 2 through decomposition of it into water and oxygen (2H 2 O 2 → O 2 + 2H 2 O) in cellular and tissue environment, which occurs widely in aerobic organisms . Catalase is a tetramer protein, and each subunit is composed of more than 500 amino acids with its heme active site deeply buried inside the protein . The decrease of catalytic activity would cause accumulation of hydrogen peroxide in vivo, leading oxidative damage to DNA and proteins .…”

Section: Introductionmentioning

confidence: 99%

Exploring adverse effects of puerarin on catalase by multiple spectroscopic investigations and docking studies in vitro

Bai¹,

Wang³

et al. 2019

J Biochem & Molecular Tox

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Puerarin belongs to one of the most familiar tradition medicines of China, but adverse effects of puerarin during the clinical treatment have been found for years, the mechanisms of which remain unclear. In this study, toxic mechanisms of puerarin on the structure and function of catalase were studied by multiple spectroscopic techniques, isothermal titration calorimetric measurement, and molecular docking methods in vitro. Results showed puerarin could inhibit the activity of catalase due to direct interactions between puerarin and catalase, resulting in conformational and functional changes of the enzyme. To be specific, puerarin statically quenched catalase fluorescence, bound into the active site channel of catalase, hindered the path of the catalytic substrate (H 2 O 2 ), affected its skeleton conformation and secondary structure, and interacted with the enzymatically related residues through hydrophobic interactions (ΔH > 0 and ΔS > 0) spontaneously (ΔG < 0). This study illustrates potential adverse effects of puerarin, which should catch more attentions during the clinical diagnosis. K E Y W O R D S adverse effect, binding mode, catalase, molecular docking, puerarin

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