Comparison of the suitability of alkaline or enzymatic sample pre-treatment for characterization of silver nanoparticles in human tissue by single particle ICP-MS

Vidmar, Janja; Buerki-Thurnherr, Tina; Loeschner, Katrin

doi:10.1039/c7ja00402h

Cited by 45 publications

(18 citation statements)

References 40 publications

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“…In contrast, it was shown that enzymatic treatment with proteinase K was more suitable for silver nanoparticles in human placental tissue, as TMAH treatment appeared to change the nanoparticles, most likely by silver ion precipitation and/or nanoparticle aggregation. 280 Please do not adjust margins Please do not adjust margins…”

Section: Sample Preparationmentioning

confidence: 99%

Analytical applications of single particle inductively coupled plasma mass spectrometry: a comprehensive and critical review

et al. 2021

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Section: Sample Preparationmentioning

confidence: 99%

Analytical applications of single particle inductively coupled plasma mass spectrometry: a comprehensive and critical review

et al. 2021

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“…This claim was drawn from published work with Au NP and not verified for NP with other compositions [33]. Caution must be used when employing enzymatic digestions for NP extraction from biological tissues due to the possibility for dissolution and possible reprecipitation [43]. Alcohols, such as methanol (MeOH), are used in a variety of organic compound extraction protocols from plant and animal tissues [44][45][46][47].…”

Section: Supplementary Informationmentioning

confidence: 99%

Methanol-based extraction protocol for insoluble and moderately water-soluble nanoparticles in plants to enable characterization by single particle ICP-MS

et al. 2020

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The detection and characterization of soluble metal nanoparticles in plant tissues are an analytical challenge, though a scientific necessity for regulating nano-enabled agrichemicals. The efficacy of two extraction methods to prepare plant samples for analysis by single particle ICP-MS, an analytical method enabling both size determination and quantification of nanoparticles (NP), was assessed. A standard enzyme-based extraction was compared to a newly developed methanol-based approach. Au, CuO, and ZnO NPs were extracted from three different plant leaf materials (lettuce, corn, and kale) selected for their agricultural relevance and differing characteristics. The enzyme-based approach was found to be unsuitable because of changes in the recovered NP size distribution of CuO NP. The MeOH-based extraction allowed reproducible extraction of the particle size distribution (PSD) without major alteration caused by the extraction. The type of leaf tissue did not significantly affect the recovered PSD. Total metal losses during the extraction process were largely due to the filtration step prior to analysis by spICP-MS, though this did not significantly affect PSD recovery. The methanol extraction worked with the three different NPs and plants tested and is suitable for studying the fate of labile metal-based nano-enabled agrichemicals.

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“…Enzymatic digestion with Proteinase K at an enzyme concentration of around 4 µg Proteinase K per mg wet tissue resulted in a satisfactory size distribution but a 60% lower recovery rate of gold nanoparticles from rat spleen in comparison to alkaline digestion with tetramethyl ammonium hydroxide (TMAH) [17]. In contrast, silver nanoparticles were satisfactorily recovered from human placenta tissue at an enzyme concentration of 60 µg Proteinase K per mg wet tissue [18] and from chicken meat at an enzyme concentration of 4 µg Proteinase K per mg wet tissue [19]. Another study was able to make successful use of alkaline digestion for both gold and silver nanoparticle extraction from ground beef, Daphnia magna, and Lumbriculus variegatus [20].…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS

Noireaux¹,

Grall

Hullo

et al. 2019

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Gold nanoparticles (AuNPs) are increasingly studied for cancer treatment purposes, as they can potentially improve both control and efficiency of the treatment. Intensive research is conducted in vitro on rodent and human cell lines to objectify the gain of combining AuNPs with cancer treatment and to understand their mechanisms of action. However, using nanoparticles in such studies requires thorough knowledge of their cellular uptake. In this study, we optimized single particle ICPMS (sp-ICPMS) analysis to qualify and quantify intracellular AuNP content after exposure of in vitro human breast cancer cell lines. To this aim, cells were treated with an alkaline digestion method with 5% TMAH, allowing the detection of gold with a yield of 97% on average. Results showed that under our experimental conditions, the AuNP size distribution appeared to be unchanged after internalization and that the uptake of particles depended on the cell line and on the exposure duration. Finally, the comparison of the particle numbers per cell with the estimates based on the gold masses showed excellent agreement, confirming the validity of the sp-ICPMS particle measurements in such complex samples.

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Comparison of the suitability of alkaline or enzymatic sample pre-treatment for characterization of silver nanoparticles in human tissue by single particle ICP-MS

Abstract: The method using enzymatic tissue treatment followed by spICP-MS is efficient for determination of mass and number concentration and size distribution of AgNPs in human placental tissues.

Cited by 45 publications

References 40 publications

Analytical applications of single particle inductively coupled plasma mass spectrometry: a comprehensive and critical review

Analytical applications of single particle inductively coupled plasma mass spectrometry: a comprehensive and critical review

Methanol-based extraction protocol for insoluble and moderately water-soluble nanoparticles in plants to enable characterization by single particle ICP-MS

Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS

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