Comparison of the sensitivity of<i>Monilinia fructicola</i>isolates to tebuconazole in Brazil using three methods

Lichtemberg, Paulo S. F.; Zeviani, Walmes M.; Michailides, Themis J.; Mio, Louise Larissa May De

doi:10.1080/07060661.2016.1147496

Cited by 7 publications

(10 citation statements)

References 14 publications

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“…The present study showed that the frequency of tebuconazole‐resistant isolates of M. fructicola in the São Paulo areas declined steadily after the 2012/13 growing season and reached similar levels to Paraná areas in the 2015/16 growing season after the absence of DMI fungicide applications. The EC 50 of the M. fructicola isolates obtained from the different monitored growing seasons in the Paraná areas was very close to the 0.046 μg mL −1 baseline sensitivity established by Lichtemberg et al (), due to the few applications of the DMI fungicide in these areas. On the other hand, the average EC 50 found in the areas of São Paulo in the 2012/13 growing season reached a value higher than the 5.65 μg mL −1 reported by May‐De‐Mio et al () and decreased continuously until the 2015/16 growing seasons, when values were close to baseline sensitivity.…”

Section: Discussionsupporting

confidence: 80%

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Discontinuance of tebuconazole in the field restores sensitivity of Monilinia fructicola in stone fruit orchards

et al. 2019

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Demethylation inhibitor (DMI) fungicides are used to control brown rot in stone fruit worldwide. However, their specific mode of action can select resistant isolates of Monilinia fructicola. Monilinia fructicola resistant to DMI fungicides are associated with a fitness cost in the absence of selective pressure, indicating that the sensitive population can be re‐established when discontinuing the fungicide in the field. This work aimed to build up the sensitive population of M. fructicola after discontinuing the use of tebuconazole for successive crop seasons. The sensitivity of M. fructicola to tebuconazole was assessed in four commercial peach orchards in Paraná and São Paulo States from 2012/13 to 2015/16. Different fungicide programmes were used and DMI fungicides were discontinued from 2013/14. The sensitivity of M. fructicola to tebuconazole was assessed by a mycelial growth assay in vitro and by determining the frequency of the G461S mutation in the MfCYP51 gene. The isolates from Paraná had high sensitivity to the fungicide across all seasons and the frequency of the G461S mutation remained below 5%. The isolates from São Paulo were highly resistant in the 2012/13 season; however, there was a gradual decline until 2015/16. In addition, the G461S mutation frequency in Sao Paulo State was about 80% in the 2012/13 season, but reduced until it was completely undetectable in 2015/16. These results provide evidence that resistance can be managed in orchards with high selective pressure to tebuconazole after discontinuing the use of the fungicide for at least 3 years.

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Section: Discussionsupporting

confidence: 80%

“…Reduction in the sensitivity of Monilinia spp. to DMI fungicides has been reported in the USA (Luo et al , ; Chen et al , ), Brazil (May‐De‐Mio et al , ; Lichtemberg et al , ), China (Chen et al , ), Spain (Egüen et al , ) and Chile (Pereira et al , ).…”

Section: Introductionmentioning

confidence: 99%

Discontinuance of tebuconazole in the field restores sensitivity of Monilinia fructicola in stone fruit orchards

et al. 2019

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“…In addition, it has been spreading in the EU since 2001 (EFSA, 2011). In Brazil, M. fructicola was reported in all stone fruit production areas (MAY-DE MIO et al, 2011;LICHTEMBERG et al, 2016) on peaches, plums and nectarines. Detection of M. laxa in São Paulo State was performed in 2007 (SOUZA et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Detection of M. laxa in São Paulo State was performed in 2007 (SOUZA et al, 2008). From 2008 to 2011, the frequency of Monilinia species was monitored annually in the peach producing areas in Brazil and there was no spread detected of M. laxa (LICHTEMBERG et al, 2016). However, M. laxa has been detecting in imported fruit from 2013 to 2015 (unpublished data) and the impact and relevance of these remains unknown and should be investigated.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of Monilinia fructicola and M. laxa isolates from Brazil: monocyclic components of peach brown rot

2017

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“…reported after just five years of usage in São Paulo, and after 10 years of usage in Paraná and Rio Grande do Sul State (May De Mio et al 2011;Lichtemberg et al 2016b). Despite the reduction in the frequency of tebuconazole resistant isolates in Brazil (Lichtemberg et al 2016b), peach and other stone fruit growers often question the effectiveness of tebuconazole for controlling brown rot, mentioning generally low control efficacy (Paulo Lichtemberg, Personal communication).…”

Section: Introductionmentioning

confidence: 99%

Fitness costs associated with G461S mutants of Monilinia fructicola could favor the management of tebuconazole resistance in Brazil

Lichtemberg

Michailides

Puckett

et al. 2018

Trop. plant pathol.

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In this study, we investigated the sensitivity components of demethylation fungicide inhibitors (DMI-tebuconazole) for two different sensitivity genotypes of Monilinia fructicola (G461S mutants and wild-type isolates). These components included the fungicide resistance stability and fitness studied over the course of nine and five successive transfers in vitro and ex vivo, respectively, in the absence of fungicide. The results showed no effect of successive transfers on tebuconazole sensitivity of isolates with the G461S mutation for in vitro and ex vivo assays. Athough stable resistance was found, resistant isolates demonstrated a fitness disadvantage relative to the wild-type isolates. The in vitro assay revealed that wild-type isolates had higher germination rates and mycelial growth capacity; and when tested ex vivo, wild-type isolates could produce more spores and larger lesions in peach fruit. The competition studies corroborate the predicted fitness results, where resistant spores were less frequently re-isolated from two pairs of resistant and sensitive mixtures prepared at different ratios. The results obtained in this study have important implications for tebuconazole resistance management of stone-fruit orchards in Brazil and field tests are currently underway to confirm its practicality.

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Comparison of the sensitivity ofMonilinia fructicolaisolates to tebuconazole in Brazil using three methods

Cited by 7 publications

References 14 publications

Discontinuance of tebuconazole in the field restores sensitivity of Monilinia fructicola in stone fruit orchards

Discontinuance of tebuconazole in the field restores sensitivity of Monilinia fructicola in stone fruit orchards

Comparative analysis of Monilinia fructicola and M. laxa isolates from Brazil: monocyclic components of peach brown rot

Fitness costs associated with G461S mutants of Monilinia fructicola could favor the management of tebuconazole resistance in Brazil

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