Comparison of the Retention of Aliphatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Modifiers of the Binary Eluent

Abstract

The retention of aliphatic hydrocarbons with polar groups has been compared in respect to the separation selectivity changes in reversed-phase high-performance liquid chromatography with C18 stationary phase type and binary water eluent composed of methanol, acetonitrile, or tetrahydrofuran as modifiers. The changes in separation selectivity when one modifier is replaced by another in the eluent is explained, taking into consideration molecular interactions of the solutes with components of the stationary phase region, i.e., extracted modifier, and ordering of the stationary phase by the modifier.

Insight into molecular characteristics of a Chinese coal via separation, characterization, and data processing

“…In the score plot (Figure 4A), the nine subfractions are divided into three groups according to the eluent used in enrichment process. This result is related to the polarity of eluents, which can influence the distribution of enriched compounds . As discussed above, PE is good at enriching hydrocarbons, and the same results are reflected to the loading plot of PCA (Figure 4B).…”

Comparison of the Retention and Separation Selectivity of Aromatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Stationary Phases and Eluents

“…Based on these circumstances, in our previous papers we proposed an approach in which separation selectivity can be explained by molecular interactions of solutes and the component of the stationary phase when one modifier is replaced by another [39,41]. The application of this approach to the explanation of separation selectivity changes was demonstrated, among others, for aliphatic hydrocarbons with a polar group [42] and aromatic hydrocarbons with different polar groups [43] in systems with C18 stationary phase type.…”