Comparison of the response of four aerosol detectors used with ultra high pressure liquid chromatography

Hutchinson, Joseph P.; Li, Jianfeng; Farrell, William; Groeber, Elizabeth; Szücs, Roman; Dicinoski, Greg W.; Haddad, Paul R.

doi:10.1016/j.chroma.2011.01.062

Cited by 59 publications

(36 citation statements)

References 24 publications

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“…The calibration curves for all substrates do not show linear relationships. As was reported in a number of papers, 14,19,21,[25][26][27] the slopes of the calibration curves for the substrates gradually decreased with increasing concentration. The signal intensity of the corona CAD should depend on the number of the electric charge.…”

Section: Syringe Pump-corona Cad Analysismentioning

confidence: 58%

“…1,10 From the comparison of these detectors, 9,[11][12][13] the reproducibility of the response from corona CAD is better than two other detectors. 14 The sensitivity of corona CAD is comparable to that of NQAD and better than that of ELSD. [14][15][16] In addition, the response of corona CAD also more universal compared with ELSD.…”

Section: Introductionmentioning

confidence: 68%

“…14 The sensitivity of corona CAD is comparable to that of NQAD and better than that of ELSD. [14][15][16] In addition, the response of corona CAD also more universal compared with ELSD. [17][18] So far, experimental results by Górecki et al showed that the response factors obtained from nonvolatile species were very close to each other when the composition of the mobile phase was matched.…”

Section: Introductionmentioning

confidence: 68%

See 2 more Smart Citations

Effects of Densities of Brominated Flame Retardants on the Detection Response for HPLC Analysis with a Corona-charged Aerosol Detector

et al. 2015

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Section: Syringe Pump-corona Cad Analysismentioning

confidence: 58%

Section: Introductionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 68%

See 1 more Smart Citation

Effects of Densities of Brominated Flame Retardants on the Detection Response for HPLC Analysis with a Corona-charged Aerosol Detector

et al. 2015

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“…While changing detector response with organic solvent concentration has been investigated for its detrimental effect on response uniformity in gradient elution [16][17][18], high organic concentrations as used in HILIC may be advantageous for sensitivity as it should facilitate desolvation of particles in the CAD. Aerosol-based detectors are known to produce non-linear calibration curves [19], which can arise for different reasons in different detectors. For instance in the ELSD, it is due to both the non-linearity of aerosol formation and a change in detection mechanism with the size of aerosol particles [20].…”

Section: Introductionmentioning

confidence: 99%

Performance of charged aerosol detection with hydrophilic interaction chromatography

Russell

Heaton

Underwood

et al. 2015

Journal of Chromatography A

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The performance of the charged aerosol detector (CAD) was investigated using a diverse set of 29 solutes, including acids, bases and neutrals, over a range of mobile phase compositions, particularly with regard to its suitability for use in hydrophilic interaction chromatography (HILIC). Flow injection analysis was employed as a rapid method to study detector performance. CAD response was 'quasi-universal', strong signals were observed for compounds that have low volatility at typical operating (room) temperature. For relatively involatile solutes, response was reasonably independent of solute chemistry, giving variation of 12-18% RSD from buffered 95% ACN (HILIC) to 10% ACN (RP). Somewhat higher response was obtained for basic compared with neutral solutes. For cationic basic solutes, use of anionic reagents of increasing size in the mobile phase (formic, trifluoroacetic and heptafluorobutyric acid) produced somewhat increased detector response, suggesting that salt formation with these reagents is contributory. However, the increase was not stoichiometric, pointing to a complex mechanism. In general, CAD response increased as the concentration of acetonitrile in the mobile phase was increased from highly aqueous (10% ACN) to values typical in the HILIC range (80-95% ACN), with signal to noise ratios about four times higher than those for the RP range. The response of the CAD is non-linear. Equations describing aerosol formation cannot entirely explain the shape of the plots. Limits of detection (determined with a column for solutes of low k) under HILIC conditions were of the order of 1-3ng on column, which compares favourably with other universal detectors. CAD response to inorganic anions allows observation of the independent movement through the column of the cationic and anionic constituents of basic drugs, which appear to be accompanied by mobile phase counterions, even at quite high solute concentrations.

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“…The WCPC condenses water vapor onto the particles and increases their size so that they could be detected individually using a laser-based optical sensor. This detection method has a linear response to macrolides 27 , amino acids 28 , aromatic compounds 29 , vitamin E 29 , fatty acids 29 , etc. Therefore, we applied HPLC-NQAD to the quantification of TAG molecular species and confirmed the linearity of the calibration curves.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Triacylglycerol Molecular Species in Cocoa Butter Using High-Performance Liquid Chromatography Equipped with Nano Quantity Analyte Detector

Beppu¹,

Nagai²,

Yoshinaga³

et al. 2013

J. Oleo Sci.

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INTRODUCTIONThe main component of lipids is triacylglycerol TAG . TAG comprises one glycerol molecule and three fatty acid molecules 1 . As many types of fatty acids exist in nature, the combination patterns of the three fatty acids are almost infinite. The idea taking account of the combination of three fatty acids in TAG is called TAG molecular species . Fats and oils consist of many TAG molecular species and the physical properties of each TAG molecular species are different. The physical properties of TAG molecular species significantly affect their emulsification properties during food processing 2 . Consequently, it is important to develop a method of quantifying the ratio of TAG molecular species in fats and oils for complete characterization.To date, many methods have been developed to quantify TAG molecular species in fats and oils. A gas chromatography-flame ionization detector GC-FID system 3, 4 is employed in the official method for quantifying TAG molecular

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Comparison of the response of four aerosol detectors used with ultra high pressure liquid chromatography

Cited by 59 publications

References 24 publications

Effects of Densities of Brominated Flame Retardants on the Detection Response for HPLC Analysis with a Corona-charged Aerosol Detector

Effects of Densities of Brominated Flame Retardants on the Detection Response for HPLC Analysis with a Corona-charged Aerosol Detector

Performance of charged aerosol detection with hydrophilic interaction chromatography

Quantification of Triacylglycerol Molecular Species in Cocoa Butter Using High-Performance Liquid Chromatography Equipped with Nano Quantity Analyte Detector

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