Comparison of the resistance to infection of intestinal submucosa arterial autografts versus polytetrafluoroethylene arterial prostheses in a dog model

Badylak, Stephen F.; Coffey, Arthur C.; Lantz, Gary C.; Tacker, Willis A.; Geddes, L. A.

doi:10.1016/s0741-5214(94)70073-7

Cited by 145 publications

(87 citation statements)

References 22 publications

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“…It is plausible that such cells would then differentiate in response to local environmental cues, and thus contribute to tissue organization and differentiation. It has also been shown that degradation products of SIS-ECM have an antimicrobial effect, [35][36][37][38] and that without ECM degradation this effect is not observed. 39 Factors that inhibit the ECM degradation process, such as chemical crosslinking, may interfere with the biological activities that have been demonstrated by the products of ECM degradation.…”

Section: Discussionmentioning

confidence: 99%

Extracellular matrix scaffolds are repopulated by bone marrow‐derived cells in a mouse model of achilles tendon reconstruction

Zantop

Gilbert

Yoder

et al. 2006

Journal Orthopaedic Research

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167

124

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The extracellular matrix derived from porcine small intestinal submucosa (SIS-ECM), an FDA-approved material currently used clinically for rotator cuff repair, has been shown to attract bone marrow-derived cells during in vivo remodeling of a subcutaneous implant and produce chemoattractant peptides following chemical degradation in vitro. The purpose of the present study was to determine if bone marrow-derived cells participate in the long-term remodeling of the Achilles tendon in a mouse model when repaired with SIS-ECM. A 2-mm gap was produced in the Achilles tendon of 40 chimeric mice produced to express green fluorescent protein (GFP) in all of their bone marrow-derived cells. Tendons were repaired by replacing the resected section with autologous tendon tissue or with a single layer sheet of lyophilized SIS-ECM. Four animals from each treatment group were sacrificed at 1, 2, 4, 8, and 16 weeks, and sections were harvested for histologic and fluorescence microscopy. Both groups showed accumulation of GFP-expressing marrow-derived cells at the site of tendon remodeling at 1 and 2 weeks that were associated with areas of angiogenesis and inflammation. By 16 weeks, the SIS-ECM-treated group showed GFP expressing cells throughout the remodeled tendon in the absence of any inflammatory response, while the autologous tendon repair group showed no GFP expressing cells within the tendon except for occasional cells in the lumen of blood vessels. An SIS-ECM scaffold used for tendon repair recruits a population of bone marrow-derived cells that participates in the long-term remodeling process. The ability of SIS-ECM to recruit a population of marrow-derived cells to the remodeling site may alter the default mechanism of tendon healing. The involvement of these cells in the remodeling process may explain in part the process of site specific constructive remodeling as opposed to scar tissue formation when the ECM is used as a biologic scaffold for tendon reconstruction. ß

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Section: Discussionmentioning

confidence: 99%

Extracellular matrix scaffolds are repopulated by bone marrow‐derived cells in a mouse model of achilles tendon reconstruction

Zantop

Gilbert

Yoder

et al. 2006

Journal Orthopaedic Research

Self Cite

167

124

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“…Leukocytes exhibit depressed chemotactic, phagocytic, and oxidative capacities after exposure to artificial materials, and shear stress may serve to exacerbate these effects. Indeed, vascular grafts using artificial materials that are implanted in dogs are significantly more susceptible to infection than are grafts made from biological materials, both autografts or allografts (55,56). Although the mechanisms through which a cardiovascular device permits the establishment of infection are unclear, evidence suggests that artificial materials produce distinct interactions that have deleterious effects on inflammatory cell function.…”

Section: Discussionmentioning

confidence: 99%

Shear stress-induced apoptosis of adherent neutrophils: A mechanism for persistence of cardiovascular device infections

Shive

Salloum

Anderson

2000

Proc. Natl. Acad. Sci. U.S.A.

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The mechanisms underlying problematic cardiovascular deviceassociated infections are not understood. Because the outcome of the acute response to infection is largely dependent on the function of neutrophils, the persistence of these infections suggests that neutrophil function may be compromised because of cellular responses to shear stress. A rotating disk system was used to generate physiologically relevant shear stress levels (0 -18 dynes͞ cm 2 ; 1 dyne ‫؍‬ 10 N) at the surface of a polyetherurethane urea film. We demonstrate that shear stress diminishes phagocytic ability in neutrophils adherent to a cardiovascular device material, and causes morphological and biochemical alterations that are consistent with those described for apoptosis. Complete neutrophil apoptosis occurred at shear stress levels above 6 dynes͞cm 2 after only 1 h. Morphologically, these cells displayed irreversible cytoplasmic and nuclear condensation while maintaining intact membranes. Analysis of neutrophil area and filamentous actin content demonstrated concomitant decreases in both cell area and actin content with increasing levels of shear stress. Neutrophil phagocytosis of adherent bacteria decreased with increasing shear stress. Biochemical alterations included membrane phosphatidylserine exposure and DNA fragmentation, as evaluated by in situ annexin V and terminal deoxynucleotidyltransferase-mediated dUTP end labeling (TUNEL) assays, respectively. The potency of the shear-stress effect was emphasized by comparative inductive studies with adherent neutrophils under static conditions. The combination of tumor necrosis factor-␣ and cycloheximide was ineffective in inducing >21% apoptosis after 3 h. These findings suggest a mechanism through which shear stress plays an important role in the development of bacterial infections at the sites of cardiovascular device implantation.

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“…[1][2][3][4][5][6][7][8][9][10][11][12] Such biological scaffolds have been surprisingly resistant to bacterial infection, [13][14][15][16] even in clinical applications that are at high risk for bacterial contamination. [17][18][19][20] Generally, naturally occurring biomaterials such as those composed of purified collagen or intact ECM are more resistant to bacterial infection than synthetic biomaterials.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Brennan

Reing²,

Chew³

et al. 2006

Tissue Engineering

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209

133

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Biological scaffolds composed of extracellular matrix (ECM) have been shown to be resistant to deliberate bacterial contamination in preclinical in vivo studies. The present study evaluated the degradation products resulting from the acid digestion of ECM scaffolds for antibacterial effects against clinical strains of Staphylococcus aureus and Escherichia coli. The ECM scaffolds were derived from porcine urinary bladder (UBM-ECM) and liver (L-ECM). These biological scaffolds were digested with acid at high temperatures, fractionated using ammonium sulfate precipitation, and tested for antibacterial activity in a standardized in vitro assay. Degradation products from both UBM-ECM and L-ECM demonstrated antibacterial activity against both S. aureus and E. coli. Specific ammonium sulfate fractions that showed antimicrobial activity varied for the 2 different ECM scaffold types. The results of this study suggest that several different lowmolecular-weight peptides with antibacterial activity exist within ECM and that these peptides may help explain the resistance to bacterial infection provided by such biological scaffolds.

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Comparison of the resistance to infection of intestinal submucosa arterial autografts versus polytetrafluoroethylene arterial prostheses in a dog model

Cited by 145 publications

References 22 publications

Extracellular matrix scaffolds are repopulated by bone marrow‐derived cells in a mouse model of achilles tendon reconstruction

Extracellular matrix scaffolds are repopulated by bone marrow‐derived cells in a mouse model of achilles tendon reconstruction

Shear stress-induced apoptosis of adherent neutrophils: A mechanism for persistence of cardiovascular device infections

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

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