Comparison of the QuantiFERON TB Gold In-tube Assay With Tuberculin Skin Test for the Diagnosis of Latent Tuberculosis Infection Among HIV-infected and Uninfected Children

Dehority, Walter; Viani, Rolando M.; Araneta, Maria Rosario G.; Lopez, Graciano; Spector, Stephen A.

doi:10.1097/inf.0000000000001771

Cited by 5 publications

(7 citation statements)

References 25 publications

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“…A meta-analysis found that the sensitivity of ELISPOT (72%, 95 CI 62–81%) was higher than that of ELISA (61%, 95 CI 41–75%) in this population [ 57 ]. Overall, the concordance between IGRAs and the TST is medium or poor [ 66 , 67 ]. Concordance is worse in low-income countries and patients with a low level of CD4 + T-cells [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Value of Interferon-Gamma Release Assays for Tuberculosis in the Immunocompromised Population

Yang

Wang

et al. 2022

Diagnostics

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Interferon-gamma release assays (IGRAs) are widely used in the diagnosis of Mycobacterium tuberculosis (M. tuberculosis) infection by detecting interferon-γ released by previously sensitized T-cells in-vitro. Currently, there are two assays based on either enzyme-linked immunosorbent assay (ELISA) or enzyme-linked immunospot (ELISPOT) technology, with several generations of products available. The diagnostic value of IGRAs in the immunocompromised population is significantly different from that in the immunocompetent population because their results are strongly affected by the host immune function. Both physiological and pathological factors can lead to an immunocompromised situation. We summarized the diagnostic value and clinical recommendations of IGRAs for different immunocompromised populations, including peoplewith physiological factors (pregnant and puerperal women, children, and older people), as well as people with pathological factors (solid organ transplantation recipients, combination with human immunodeficiency virus infection, diabetes mellitus, end-stage renal disease, end-stage liver disease, and chronic immune-mediated inflammatory diseases). Though the performance of IGRAs is not perfect and often requires a combination with other diagnostic strategies, it still has some value in the immunocompromised population. Hopefully, the newly developed IGRAs could better target this population.

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Section: Discussionmentioning

confidence: 99%

Diagnostic Value of Interferon-Gamma Release Assays for Tuberculosis in the Immunocompromised Population

Yang

Wang

et al. 2022

Diagnostics

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“…The test’s veracity depends on the generation of an appropriate standard curve, which must cover the specific criteria established by the test. A positive result is considered when the amount of IFN- γ released in response to antigens ESAT-6, CFP 10 and TB 7.7 has a value greater than 0.35 IU/ml, according to the manufacturer’s instructions [ 11 – 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…This test detects the amount of interferon gamma (IFN-γ) released by lymphocytes in the blood of sensitized people, using the Enzyme-Linked Immunosorbent Assay (ELISA). It contains mixtures of synthetic peptides similar to those presented by MTB and absent in the Bacillus Calmette Guerin (BCG) used for vaccination: white secretory early antigen (ESAT-6), culture-filtered protein (CFP-10) [ 9 , 10 ] and in later years the TB 7.7, which reduces the number of indeterminate results and increases the diagnostic sensitivity and specificity [ 11 – 13 ]. Recently, to improve the sensitivity of the QFT-GIT in young children, or people with recent exposure, or with HIV infection, was developed the QFT-Plus that has one additional tube for the induction of cell-mediated immune responses from both CD4+ 31 and CD8+ T cells.…”

Section: Introductionmentioning

confidence: 99%

“…In international literature, only a few studies address the issue of LTBI in the pediatric population in Mexico, mainly in populations living in the border with United States [ 11 , 15 ]. For this reason, the objective of the present study is to identify the prevalence and to evaluate the concordance of the TST and QFT diagnosis tests for LTBI in a group of children with HIV-infection treated in Guadalajara, Mexico, city located in the west-center of the country, where no previous studies has issued this question.…”

Section: Introductionmentioning

confidence: 99%

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A prevalence study in Guadalajara, Mexico, comparing tuberculin skin test and QuantiFERON-TB Gold In-Tube

et al. 2022

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Background Tuberculosis (TB) is a prevalent disease throughout the world. The extent of TB illness in childhood is not clear; recent data shows that 10–20% of the cases are found in children under 15 years old. In 2017, 1 million children developed the disease, of which 9% were co-infected with HIV. Methods A cross-sectional study that analyzed 48 children diagnosed with HIV-infection in Guadalajara, Mexico. The tuberculin skin test (TST) and QuantiFERON-TB Gold In-Tube test (QFT) were performed and compared to diagnose latent TB infection (LTBI). Results The average age was 9 years old (± 4), with an age range of 1–16 years; the 6-12-year-old group predominated with 50% of cases. 27 patients (56%) were male; 83% had received the BCG vaccination and 23% had a history of being contacts of TB cases. In the study, 40 patients (83%) were without immunosuppression; seven (15%) with moderate immunosuppression, and only one patient had severe immunodeficiency. Overall, 3 of the 48 children (6.2%) had a positive TST, while 8 out of 48 (16.6%) had a positive QFT. The concordance between the two tests was 89.6% (43/48) with Kappa = 0.5 (95% CI, 0.14–0.85). Conclusions The QFT test represents an opportunity in the diagnosis of LTBI, particularly in pediatric HIV- patients. This is the first study that compares the two tests (TST and QFT) in children with HIV-infection in Guadalajara, Mexico.

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“…The QFT-GIT has been reported to have higher sensitivity, specificity and be more attractive than the tuberculin skin test (TST) [4][5][6][7][9][10][11] which may result in interpretation errors [5,6,11], including the need of two clinic visits. Collecting blood samples may not frequently encounter difficulties compared with other specimens collection and QFT-GIT can provide rapid results, within two days [6].…”

Section: Introductionmentioning

confidence: 99%

Performance of a lymphocyte t interferon gamma test (Quantiferon-TB gold in tube) in the diagnosis of active tuberculosis in HIV-infected children

et al. 2020

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Objective Evaluate the performance of QuantiFERON ® -TB Gold In-Tube test (QFT-GIT), to improve the diagnosis of active tuberculosis (TB) in Human Immuno-Deficiency Virus (HIV)-infected children. Method Sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV) of QFT-GIT were assessed in 58/63 HIV-infected children who were suspected of having TB. Results Sensitivity of QFT-GIT was 20.69%, specificity 96.55%, PPV/NPV respectively 85.71% and 54.90%. Conclusion QFT-GIT appears to be of little contribution to the diagnosis of active TB in children living with HIV in a TB-endemic country.

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Comparison of the QuantiFERON TB Gold In-tube Assay With Tuberculin Skin Test for the Diagnosis of Latent Tuberculosis Infection Among HIV-infected and Uninfected Children

Abstract: A moderate correlation exists between TST and QTF-GIT among HIV-infected and uninfected children with preserved immune function in an area of moderate tuberculosis endemicity.

Cited by 5 publications

References 25 publications

Diagnostic Value of Interferon-Gamma Release Assays for Tuberculosis in the Immunocompromised Population

Diagnostic Value of Interferon-Gamma Release Assays for Tuberculosis in the Immunocompromised Population

A prevalence study in Guadalajara, Mexico, comparing tuberculin skin test and QuantiFERON-TB Gold In-Tube

Performance of a lymphocyte t interferon gamma test (Quantiferon-TB gold in tube) in the diagnosis of active tuberculosis in HIV-infected children

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