Comparison of the Protoscolocidal Effectiveness of Hypertonic Saline, Povidone—Iodine and Albendazole Solutions in an Experimental Lung Hydatid Cyst Model

Abstract

Secondary hydatidosis is an important problem encountered during the surgical treatment of hydatid cysts. This study describes an experimental model of secondary hydatidosis by cyst inoculation, used to explore whether simultaneous inoculation of protoscolocidal agents could prevent secondary hydatidosis. Fertile cyst fluid was injected into the pleural space of rabbits alone (group 1, n = 8), and in combination with 2% albendazole solution (group 2, n = 8), 20% hypertonic saline (group 3, n = 8) or 10% povidone-iodine (group 4, n = 8). Computed tomography imaging of the thorax, indirect haemagglutination (IHA) titres and eosinophil counts were used to determine cyst development. After 16 months, three control rabbits had pneumothorax, seven had cysts and four had parenchymal nodules. Histopatho logical investigation of nodules revealed 87.5% cyst formation. Pleural thickening was observed in rabbits from all groups. Cyst formation rates, IHA titres and eosinophilia counts were higher in group 1 than in groups 2 -4. This study demonstrated the experimental formation of secondary hydatidosis and found that topical protoscolocidal agents were beneficial in preventing cyst recurrence.

Improved experimental model of hepatic cystic hydatid disease resembling natural infection route with stable growing dynamics and immune reaction

“…Group B was superior for research due to its low dose of infection and predictable cyst development as well as better normal distribution. It will benefit experimenters to observe the in vivo efficacy of new treatments against hydatid without the need for sacrificing the mouse[ 12 - 15 ].…”

Progress in the pharmacological treatment of human cystic and alveolar echinococcosis: Compounds and therapeutic targets

“…In addition, 150 mg·kg -1 ABZ administered for 10 consecutive days from the time of infection was demonstrated to completely inhibit cyst growing [ 88 ]. ABZ has also been tested in the treatment of secondary pleural hydatidosis in rabbits [ 90 ]. In this animal model intra-thoracic administration of 2% ABZ as a single dose was simultaneously conducted with the injection of hydatid cyst fluid containing fertile protoscoleces into the pleural cavity of experimentally infected rabbits.…”

“…H 2 O 2 and povidone-iodine have shown scolicidal activity in vitro at 4% and 10%, respectively [ 73 , 90 ], but have been more frequently used peri-operatively covering the peri-cystic interventional area with patches soaked with diluted solutions of one of these compounds. Due to their toxicity, the intra-cyst use of H 2 O 2 and povidone-iodine has been performed in very few cases [ 135 , 136 ] and its general use for intra-operative interventions cannot be recommended.…”