Comparison of the proteomes of three yeast wild type strains: CEN.PK2, FY1679 and W303

Rogowska-Wrzesińska, Adelina; Larsen, Peter Mose; Blomberg, Anders; Görg, Angelika; Roepstorff, Peter; Norbeck, Joakim; Fey, Stephen J.

doi:10.1002/cfg.94

Cited by 40 publications

(29 citation statements)

References 49 publications

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“…Because we did not detect an accumulation of ERCs, we measured the average lifespan in our hho1 Δ cells to address the possibility that the shortened lifespan of hho1Δ mutants is a strain‐dependent phenotype. Different phenotypes in W303 and S288C strains with the same mutant allele have been observed previously (for example, see Rogowska‐Wrzesinska et al ., 2001). We analysed lifespan in wild‐type, hho1Δ and sir2Δ cells of the S288C genetic background (Fig.…”

Section: Resultssupporting

confidence: 67%

Linker histone H1 represses recombination at the ribosomal DNA locus in the budding yeast Saccharomyces cerevisiae

Mueller

Elfline

et al. 2008

Molecular Microbiology

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SummarySeveral epigenetic phenomena occur at ribosomal DNA loci in eukaryotic cells, including the silencing of Pol I and Pol II transcribed genes, silencing of replication origins and repression of recombination. In Saccharomyces cerevisiae, studies focusing on the silencing of Pol II transcription and genetic recombination at the ribosomal DNA locus (rDNA) have provided insight into the mechanisms through which chromatin and chromatin-associated factors regulate gene expression and chromosome stability. The core histones, H2A, H2B, H3 and H4, the fundamental building blocks of chromatin, have been shown to regulate silent chromatin at the rDNA; however, the function of the linker histone H1 has not been well characterized. Here, we show that S. cerevisiae histone H1 represses recombination at the rDNA without affecting Pol II gene silencing. The most highly studied repressor of recombination at the rDNA is the Silent information regulator protein Sir2. We find that cells lacking histone H1 do not exhibit a premature-ageing phenotype nor do they accumulate the rDNA recombination intermediates and products that are found in cells lacking Sir2. These results suggest that histone H1 represses recombination at the rDNA by a mechanism that is independent of the recombination pathways regulated by Sir2.

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Section: Resultssupporting

confidence: 67%

Linker histone H1 represses recombination at the ribosomal DNA locus in the budding yeast Saccharomyces cerevisiae

Mueller

Elfline

et al. 2008

Molecular Microbiology

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“…A few other studies have shown that different S. cerevisiae genetic backgrounds result in significant physiological differences, e.g. in lipid metabolism (Daum et al 1999) or protein expression (Rogowska-Wrzesinska et al 2001). Another good example is the comparison of ENA/PMR2 loci encoding the alkali-metal cation ATPase (Wieland et al 1995;Daran-Lapujade et al 2003).…”

Section: Resultsmentioning

confidence: 98%

Saccharomyces cerevisiae BY4741 and W303-1A laboratory strains differ in salt tolerance

2010

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“…Variant D differs in a single nucleotide substitution from variant A and is present only in laboratory strains S288c and its derivative W303 (Rogowska-Wrzesinska et al 2001). S288c, the most popular genetic background in yeast research laboratories, is a derivative of a natural heterothallic diploid strain isolated from rotting figs in California in 1938 (Mortimer and Johnston 1986).…”

Section: Frequency and Origin Of The Translocation (Viii;xvi) In S Cmentioning

confidence: 99%

Molecular Characterization of a Chromosomal Rearrangement Involved in the Adaptive Evolution of Yeast Strains

Pérez‐Ortín

Querol²,

Puig³

et al. 2002

Genome Res.

240

273

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Wine yeast strains show a high level of chromosome length polymorphism. This polymorphism is mainly generated by illegitimate recombination mediated by Ty transposons or subtelomeric repeated sequences. We have found, however, that the SSU1-R allele, which confers sulfite resistance to yeast cells, is the product of a reciprocal translocation between chromosomes VIII and XVI due to unequal crossing-over mediated by microhomology between very short sequences on the 5Ј upstream regions of the SSU1 and ECM34 genes. We also show that this translocation is only present in wine yeast strains, suggesting that the use for millennia of sulfite as a preservative in wine production could have favored its selection. This is the first time that a gross chromosomal rearrangement is shown to be involved in the adaptive evolution of Saccharomyces cerevisiae.[The sequence data from this study have been submitted to EMBL under accession nos. AF239757, AF239758, and AJ458340-AJ458367. The following individual kindly provided reagents, samples, or unpublished information as indicated in the paper: N. Goto-Yamamoto.] The unaware use of yeast for winemaking by the first agricultural civilizations has been reported as far back as 7400 years ago. Until the middle of the last millennium, wines were mainly produced around the Mediterranean Sea and the Caucasus. Since then, winemaking has spread with the European colonizers throughout the temperate regions of the world (Pretorius 2000).Although different genera and species of yeasts are found in musts, the species Saccharomyces cerevisiae is mainly responsible for the transformation of musts into wines. The origin of S. cerevisiae is controversial. Some authors propose that this species is a "natural" organism present in plant fruits (Mortimer and Polsinelli 1999). Others argue that S. cerevisiae is a domesticated species originated from its closest relative S. paradoxus, a wild species found all around the world (Vaughan-Martini and Martini 1995). This debate is important in postulating the original genome of S. cerevisiae and how the strong selective pressure applied since its first unconscious use in controlled fermentation processes has reshaped it. Useful phenotypic traits such as fast growth in sugar-rich media, high alcohol production and tolerance, and good flavor production selected for billions of generations have had strong influences on the S. cerevisiae genome.In contrast to most S. cerevisiae strains used in the laboratory, which are either haploid or diploid and have a constant chromosome electrophoretic profile, wine yeast strains are mainly diploid, aneuploid, or polyploid, homothallic, and . Their exacerbated capacity to reorganize its genome by chromosome rearrangements such as Ty-promoted chromosomal translocations (Longo and Vézinhet 1993;Rachidi et al. 1999), mitotic crossing-over (Aguilera et al. 2000), and gene conversion (Puig et al. 2000) promotes a faster adaptation to environmental changes than spontaneous mutations, which occur at comparatively very low rat...

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Comparison of the proteomes of three yeast wild type strains: CEN.PK2, FY1679 and W303

Cited by 40 publications

References 49 publications

Linker histone H1 represses recombination at the ribosomal DNA locus in the budding yeast Saccharomyces cerevisiae

Linker histone H1 represses recombination at the ribosomal DNA locus in the budding yeast Saccharomyces cerevisiae

Saccharomyces cerevisiae BY4741 and W303-1A laboratory strains differ in salt tolerance

Molecular Characterization of a Chromosomal Rearrangement Involved in the Adaptive Evolution of Yeast Strains

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