Comparison of the properties of collagen extracted from dried jellyfish and dried squid

Abstract

With increased recent interest in the utilization of industrial by-products, finding different sources, optimizing extracting conditions and characterization of collagen extracts have recently become important research topics. This study addresses the isolation of acid-soluble and pepsin-soluble forms of collagen from dried jellyfish and squid, and their partial characterization. The properties of these proteins have been studied and a comparison made of the protein patterns of collagen extracted from marine organisms with those from other organisms, to determine which collagen subtypes are present, and in what proportions. Pepsin-soluble collagen (PSC) from dried jellyfish and dried squid contained a collagen form classified as type I, of molecular composition comparable with that of collagen type I from rat tail. Peptide maps of collagens digested by achromopeptidase were slightly different, indicating some differences in amino acid sequence or conformation. The collagen showed high solubility at acidic pH (4-5) but its solubility markedly decreased in the presence of sodium chloride (NaCl) up to 2%. Collagen type I from dried jellyfish and dried squid could be a useful alternative to mammalian collagen, with potential use in the biomedical, pharmaceutical and nutraceuticals industries.

Characterization of Purified Collagen from Marine Squid Uroteuthis Duvauceli

“…The solutions were centrifuged at 20,000 g for 30 min and the protein content of the supernatant determined. Protein solubility was calculated using the following equation 11 .…”

“…The mixture was stirred for 30 min, followed by centrifugation at 20,000 g for 30 min. Protein content in the supernatant was measured and the relative solubility calculated using the following equation 11 .…”

Collagen from Marine Biological Sources and Medical Applications

“…The marine environment serves as an intersting source of collagen and bioactive substances that are utilized in different fields including pharmaceutical, cosmetic, and food industry . Marine collagen can be categorized into two depending on the source: collagen isolated from invertebrate animals including cuttlefish, sea anemone, prawn, star fish, jellyfish, sponges, sea urchin, octopus, squid, constitute invertebrate source of marine collagen. Another category of marine collagen falls under marine vertebrate source which include fish and marine mammals.…”

“…Enzyme inactivation can also be done by dialyzing the extract solution against buffer solutions, e.g . sodium phosphate buffer for acidic extracts . The extract solution is salted out and the resulting precipitate is collected by centrifugation or ultrafiltration and dialyzed against buffer solution followed by distilled water and then lyophilized.…”

Pemanfaatan Kolagen Laut dalam Sistem Penghantaran Obat

“…Berdasarkan sumbernya, kolagen laut dikategorikan menjadi dua yaitu kolagen yang diisolasi dari hewan invertebrata seperti sotong, anemon laut, udang, ikan bintang, ubur-ubur 2,3,4,5 , spons 12 , bulu babi, gurita, dan cumi-cumi 13 . Kategori lainnya adalah kolagen laut yang bersumber dari vertebrata laut yang mencakup ikan dan mamalia laut.…”