Comparison of the precision of internal and external standard calibration in an interlaboratory liquid chromatographic study

Pauls, R. E.; McCoy, Robert W.

doi:10.1002/jhrc.1240091015

Cited by 9 publications

(3 citation statements)

References 5 publications

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“…There has been some discussion in the literature with regard to the use of internal standards [4]. The lower limit of quantification (LLOQ)is 10pgmL 1 using l mL of plasma, with a validated dynamic range of 10 to 500 pgmL 1.…”

Section: Discussionmentioning

confidence: 99%

Analysis of CP-122,288 at femto-molar levels from human plasma using solid-supported extraction and HPLC column switching

2000

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SummaryA sensitive and specific method for the analysis of CP-122,288 in p Jasma has been developed and validated using column-switching HPLC with fluorescence detection. Solid-phase extraction (SPE) cartridges (Chem-Elut) are used to extract analyte and internal standard from human plasma samples. The extracts are analysed by heart-cutting from one analytical HPLC column to a second column with fluorescence detection using excitation and emission wavelengths of 225 and 350 nm respectively after post column UV irradiation at 254 nm. The method has a lower limit of quantification (LLOQ) of 10 pg mL 1 and is linear over a fift,/-fold range, u p to 500 pg mL 1. An internal standard 638) is used which is a close chemical analogue of the compound. This validated method has been used extensively for the analysis of CP-122,288 in plasma samples from clinical trials.

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Section: Discussionmentioning

confidence: 99%

Analysis of CP-122,288 at femto-molar levels from human plasma using solid-supported extraction and HPLC column switching

2000

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“…The successful use of an internal standard depends on the existence of a high correlation between the peak areas (heights) of the analytes and the internal standard for the complete analytical procedure and their being lower variability in the internal standard area (height) compared to those of the analytes [291,292]. The successful use of an internal standard depends on the existence of a high correlation between the peak areas (heights) of the analytes and the internal standard for the complete analytical procedure and their being lower variability in the internal standard area (height) compared to those of the analytes [291,292].…”

Section: Properties Required Of An Ideal Internal Standardmentioning

confidence: 99%

Fundamental Relationships of Chromatography

Poole

1991

Chromatography Today

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“…Internal standards would correct for this if added to the unknown at the beginning of the sample preparation. 4 The measurement uncertainty based on the Guide of Uncertainty Measurement (GUM) 5 depends on the proper combination of various sources of uncertainty and therefore the mathematical model used to calculate the appropriate analyte in question, from a calibration curve with external or internal standard. 6,7 …”

Section: Introductionmentioning

confidence: 99%

Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysis

Oliveira¹,

Müller²,

Abad³

et al. 2010

Quím. Nova

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, 8.000, 94930-000 Cachoeirinha -RS, BrasilRecebido em 22/9/09; aceito em 30/11/09, publicado na web em 8/4/10Traditionally, in the cigarettes industry, the determination of ammonium ion in the mainstream smoke is performed by ion chromatography. This work studies this determination and compares the results of this technique with the use of external and internal standard calibration. A reference cigarette sample presented measurement uncertainty of 2.0 mg/cigarette and 1.5 mg/cigarette, with external and internal standard, respectively. It is observed that the greatest source of uncertainty is the bias correction factor and that it is even more significant when using external standard, confirming thus the importance of internal standardization for this correction.

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Comparison of the precision of internal and external standard calibration in an interlaboratory liquid chromatographic study

Cited by 9 publications

References 5 publications

Analysis of CP-122,288 at femto-molar levels from human plasma using solid-supported extraction and HPLC column switching

Analysis of CP-122,288 at femto-molar levels from human plasma using solid-supported extraction and HPLC column switching

Fundamental Relationships of Chromatography

Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysis

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