Analysis of CP-122,288 at femto-molar levels from human plasma using solid-supported extraction and HPLC column switching

Abstract

SummaryA sensitive and specific method for the analysis of CP-122,288 in p Jasma has been developed and validated using column-switching HPLC with fluorescence detection. Solid-phase extraction (SPE) cartridges (Chem-Elut) are used to extract analyte and internal standard from human plasma samples. The extracts are analysed by heart-cutting from one analytical HPLC column to a second column with fluorescence detection using excitation and emission wavelengths of 225 and 350 nm respectively after post column UV irradiation at 254 nm. The method has a lower limit of quantification (LLOQ) of 10 pg mL 1 and is linear over a fift,/-fold range, u p to 500 pg mL 1. An internal standard 638) is used which is a close chemical analogue of the compound. This validated method has been used extensively for the analysis of CP-122,288 in plasma samples from clinical trials.

Determination of a potent urokinase-type plasminogen activator, UK-356,202, in plasma at pg/mL levels using column-switching HPLC and fluorescence detection

“…Classical detection techniques such as fluorescence have generally become limited to assays where the compounds are of high molecular weight [4] or where HPLC-MS/MS is not sensitive enough [5]. In other cases it still offers a cheaper and more convenient alternative [6,7].…”