Comparison of the platelet‐rich plasma and buffy coat protocols for preparation of canine platelet concentrates

Abstract

TitleComparison of the platelet-rich plasma and buffy coat protocols for preparation of canine platelet concentrates Permalink https://escholarship.org/uc/item/9px7k8tz Veterinary Clinical Pathology, 43(4) Journal

Equine platelet concentrate preparation and validation

“…All the units of PC reached the target of ≥5.5 × 10 10 PLTs/unit and had a plasma pH ≥6.2 at the end of the 5‐day storage time, thus meeting the American Association of Blood Banks standards for human PC . Interestingly, when PRP and BC protocols were compared for preparation of canine PC, none of the PC units met this requirement . Another study evaluating a PRP method for production of canine PC demonstrated a mean PLT yield of 8 × 10 10 per PC unit .…”

“…6 Interestingly, when PRP and BC protocols were compared for preparation of canine PC, none of the PC units met this requirement. 4 Another study evaluating a PRP method for production of canine PC demonstrated a mean PLT yield of 8 × 10 10 per PC unit. 7 Minimizing both WBC and RBC contamination in PC is important as RBC and WBC can enhance PLT aggregation and contribute to PLT activation.…”

“…In humans, the PRP method is used more commonly in North America and the BC technique is used more often in Europe. In a study comparing these 2 methods to produce canine PC, PRP‐PC had less white blood cell (WBC) and RBC contamination and superior PLT function compared to the BC‐PC . The objectives of this study were to test and validate the methodology for production of an equine PRP‐PC product, including assessment of PLT, WBC, and RBC concentrations, in vitro PLT function, microbial growth and markers of platelet storage lesion (PSL) throughout storage.…”

Comparison of equine platelet function and survival in whole blood collected in acid‐citrate‐dextrose solution or citrate‐phosphate‐dextrose‐adenine solution

“…The mean lactate, glucose, and bicarbonate concentrations were consistently higher in CPDA compared to ACD. The bicarbonate concentrations significantly decreased after one hour in both anticoagulants suggesting some storage‐associated PLT damage in both anticoagulants, and higher lactate concentrations and lower bicarbonate and glucose concentrations are suggestive of PLT storage injury . Glucose is required for PLT metabolism, but can be associated with increased lactate production and decreased pH triggering PLT storage lesion.…”

“…Platelets are reactive cells that can be irreversibly activated during processing and storage . Platelet activation, and subsequent cell damage, can be induced by shear stress, contact with charged surfaces, changes in temperature, and decreased oxygen availability.…”