Comparison of equine platelet function and survival in whole blood collected in acid‐citrate‐dextrose solution or citrate‐phosphate‐dextrose‐adenine solution

Bozorgmanesh, Rana; Sutton‐Burges, Julie W.; Tablin, Fern

doi:10.1111/vcp.12491

Cited by 4 publications

(6 citation statements)

References 13 publications

(15 reference statements)

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“…Herein CPD-A was used in methods 1 and 3, and sodium citrate in method 2. There is no data to suggest that either one of the anticoagulants used in the present study is superior to another; however, both were superior to acid-citrate-dextrose solution in two studies [ 42 , 43 ]. Certainly, in this study, the platelet viability and clumps were not affected by the method used.…”

Section: Discussionmentioning

confidence: 82%

Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma

Segabinazzi

Podico

Rosser

et al. 2021

Animals

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In light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (n = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (p < 0.05), and the latter also had greater contamination with WBC than the others (p < 0.001). Platelet viability was similar across treatments (p > 0.05). Cooling for 24 h did not affect platelet counts in all methods (p > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (p = 0.04), and agglutination increased over time in all methods (p < 0.001). The three methods increased (1.8–5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy.

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Section: Discussionmentioning

confidence: 82%

Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma

Segabinazzi

Podico

Rosser

et al. 2021

Animals

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“…The administration of blood products to horses is a life‐saving procedure not devoid of risk 1‐8 . The incidence of transfusion‐associated adverse reactions is approximately 16% in horses 9 .…”

Section: Introductionmentioning

confidence: 99%

Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage

Miglio,

Rocconi,

Cremonini

et al. 2024

Veterinary Internal Medicne

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BackgroundUnderstanding of the biochemical and morphological lesions associated with storage of equine blood is limited.ObjectiveTo demonstrate the temporal sequences of lipid and metabolic profiles of equine fresh and stored (up to 42 days) and leukoreduced packed red blood cells (LR‐pRBC) and non‐leukoreduced packed RBC (nLR‐pRBC).AnimalsPacked RBC units were obtained from 6 healthy blood donor horses enrolled in 2 blood banks.MethodsObservational study. Whole blood was collected from each donor using transfusion bags with a LR filter. Leukoreduction pRBC and nLR‐pRBC units were obtained and stored at 4°C for up 42 days. Sterile weekly sampling was performed from each unit for analyses.ResultsRed blood cells and supernatants progressively accumulated lactate products while high‐energy phosphate compounds (adenosine triphosphate and 2,3‐Diphosphoglycerate) declined. Hypoxanthine, xanthine, and free fatty acids accumulated in stored RBC and supernatants. These lesions were exacerbated in non‐LR‐pRBC.Conclusion and Clinical ImportanceLeukoreduction has a beneficial effect on RBC energy and redox metabolism of equine pRBC and the onset and severity of the metabolic storage lesions RBC.

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“…The Multiplate whole blood impedance aggregometer is an established clinical and research instrument for evaluating platelet function in human patients. Equine platelet function has been examined previously using flow cytometry 4 and impedance aggregometry 5,6 . Multiplate technology has been validated for platelet function testing in horses by a previous study 7 and employed to assess the inhibitory effects of clopidogrel 8 and aspirin 9 therapy on equine platelet aggregation.…”

Section: Introductionmentioning

confidence: 99%

“…Equine platelet function has been examined previously using flow cytometry 4 and impedance aggregometry. 5,6 Multiplate technology has been validated for platelet function testing in horses by a previous study 7 and employed to assess the inhibitory effects of clopidogrel 8 and aspirin 9 therapy on equine platelet aggregation. Metabolic acidosis is a common acid-base disturbance that can occur in ill horses due to tissue hypoxia or ischemia, resulting in increased anaerobic metabolism and lactic acid production.…”

Section: Introductionmentioning

confidence: 99%

Effect of lactic acid addition to equine whole blood on platelet aggregation measured by impedance aggregometry

Lanier

Taintor

Christopherson

et al. 2022

Veterinary Clinical Pathol

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Background Acidemia in sick or injured horses is often due to lactic acid accumulation. Alterations in platelet function and hemostasis are among numerous deleterious effects caused by decreased physiologic pH. Objectives We aimed to evaluate the effect of hyperlactatemia and resultant acidemia on platelet aggregation in equine whole blood using impedance aggregometry. Methods Platelet aggregation was measured using the Multiplate analyzer in whole blood from 34 healthy horses at baseline and after in vitro addition of lactic acid to adjust the pH. Platelet aggregation of each sample was quantified by the area under the curve measurement reported by the Multiplate system. The association between platelet aggregation and pH was analyzed using a linear mixed‐effects model. The association of baseline platelet aggregation with hematocrits (Hcts), white blood cell (WBC) counts, and platelet counts was evaluated using Pearson's correlations. Results There was a significant association between acidemia and decreased platelet aggregation. No significant correlations were detected between platelet aggregation and Hct, WBC count, or platelet count. Platelet aggregation measured in healthy horses using the Multiplate analyzer showed substantial variation between animals. Conclusions Acidemia caused by the addition of lactic acid to equine whole blood was associated with a mild though statistically significant decrease in platelet aggregation. In conjunction with other factors, this change may contribute to morbidity‐related disorders of hemostasis, although its precise clinical relevance is uncertain.

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Comparison of equine platelet function and survival in whole blood collected in acid‐citrate‐dextrose solution or citrate‐phosphate‐dextrose‐adenine solution

Cited by 4 publications

References 13 publications

Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma

Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma

Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage

Effect of lactic acid addition to equine whole blood on platelet aggregation measured by impedance aggregometry

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