Comparison of the performance of targeted mitochondrial antioxidant mitoquinone and non-targeted antioxidant pentoxifylline in improving rooster sperm parameters during freezing and thawing

Nazari, Mahdi; Kia, Hossein Daghigh; Mehdipour, Mahdieh; Najafi, Abouzar

doi:10.1016/j.psj.2022.102035

Cited by 4 publications

(1 citation statement)

References 46 publications

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“…Other studies have also shown that, although PTX could increase sperm motility, it did not have a significant effect on the acrosomal reaction of the thawed spermatozoa. In this study, they reasoned that PTX could not preserve the sperm membrane integrity, which was needed for the initiation of the AR but, when mitoquinone was added, sperm membrane integrity improved significantly (Nazari et al, 2022).…”

Section: Discussionmentioning

confidence: 93%

The effect of pentoxifylline and calcium ionophore treatment on sperm cell biology in oligoasthenoteratozoospermia samples

Mahaldashtian

Khalili

Vatanparast

et al. 2022

Zygote

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Summary The objective of this study was to assess the effects of pentoxifylline (PTX) and Ca2+ ionophore (CI) A12387 treatment on some biological characteristics of sperm cells in oligoasthenoteratozoospermia (OAT) patients. After processing, each sample was divided into four groups: 1, control; 2, exposed to 3.6 mM PTX; 3, exposed to 5 μm calcium ionophore (CI); and 4, exposed to both PTX and CI; 30 min at 37°C. Sperm motility was measured before and after preparation. Acrosome reaction (AR), status of sperm vacuoles, mitochondrial membrane potential (MMP) and DNA fragmentation were assessed using PSA-FITC staining, motile sperm organelle morphology examination (MSOME), JC-1 staining and sperm chromatin dispersion (CSD) test, respectively. Treatment with PTX and CI led to increased and decreased sperm motility, respectively (P < 0.05). Furthermore, vacuole status and rates of sperm DNA fragmentation were not significantly different among groups (P > 0.05). Moreover, the data showed that the rates of AR and disrupted MMP were significantly different between groups (P < 0.05). In conclusion, in vitro application of PTX not only did not have any adverse effects on sperm cell biology characteristics, but also can rectify the harmful effect of CI.

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Section: Discussionmentioning

confidence: 93%