Effects of mitoquinone (MitoQ) supplementation during boar semen cryopreservation on sperm quality, antioxidant status and mitochondrial proteomics

Shi, Lei; Zhang, Yan; Huang, Xiaoyu; Shi, Mingyue; Sun, Di; Zhang, Yanwei; Li, Wenxin; Jin, Tianyu; Feng, Jingjuan; Xing, Jiandong; Li, Bugao; Cao, Guoqing

doi:10.1016/j.anireprosci.2022.107099

Cited by 3 publications

(3 citation statements)

References 52 publications

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“…Mitochondria are the main source of sperm energy and the main site of ROS production [ 27 ]. ROS can damage sperm mitochondria, alter MMP, induce mitochondrial dysfunction, and further affect sperm energy metabolism [ 28 ]. During sperm preservation, the AMPK signaling pathway can significantly improve sperm preservation quality and antioxidant capacity [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics Analysis of Sodium Salicylate Improving the Preservation Quality of Ram Sperm

Bai,

Zhang,

Shen

et al. 2023

Molecules

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The aim of this study was to investigate the effects of sodium salicylate (SS) on the preservation and metabolic regulation of sheep sperm. Under 4 °C low-temperature conditions, SS (at 10 µM, 20 µM, 30 µM, and 50 µM) was added to the semen diluent to detect sperm motility, plasma membrane, and acrosome integrity. Based on the selected optimal concentration of SS (20 µM), the effects of 20 µM of SS on sperms’ antioxidant capacity and mitochondrial membrane potential (MMP) were evaluated, and metabolomics analysis was conducted. The results showed that on the 20th day of low-temperature storage, the sperm motility of the 20 µM SS group was 62.80%, and the activities of catalase (CAT) and superoxide dismutase (SOD) were significantly higher than those of the control group (p < 0.01). The content of Ca2+, reactive oxygen species (ROS), and malondialdehyde (MDA) were significantly lower than those of the control group (p < 0.01), and the total antioxidant capacity (T-AOC) was significantly higher than that of the control group (p < 0.05); mitochondrial activity and the total cholesterol (TC) content were significantly higher than those in the control group (p < 0.01). An ultrastructural examination showed that in the SS group, the sperm plasma membrane and acrosome were intact, the fibrous sheath and axoneme morphology of the outer dense fibers were normal, and the mitochondria were arranged neatly. In the control group, there was significant swelling of the sperm plasma membrane, rupture of the acrosome, and vacuolization of mitochondria. Using metabolomics analysis, 20 of the most significant differential metabolic markers were screened, mainly involving 6 metabolic pathways, with the amino acid biosynthesis pathway being the most abundant. In summary, 20 µM of SS significantly improved the preservation quality of sheep sperm under low-temperature conditions of 4 °C.

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Section: Discussionmentioning

confidence: 99%

Metabolomics Analysis of Sodium Salicylate Improving the Preservation Quality of Ram Sperm

Bai,

Zhang,

Shen

et al. 2023

Molecules

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show abstract

“…The antioxidant status of sperm samples was evaluated using the same methods described previously (Shi et al, 2022). Total antioxidant capacity (T‐AOC), malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH‐Px) activity, hydrogen peroxide (H 2 O 2 ) content, and catalase (CAT) activity were determined using the commercial biochemical reagent kit (Jiancheng Bioengineering Institute, Nanjing, China; Product No.…”

Section: Methodsmentioning

confidence: 99%

“…The antioxidant status of sperm samples was evaluated using the same methods described previously (Shi et al, 2022)…”

Section: Determination Of Sperm Antioxidant Capacitymentioning

confidence: 99%

Exogenous spermidine effectively improves the quality of cryopreserved boar sperm

Li,

Wang,

Guo

et al. 2023

Animal Science Journal

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Boar sperm are less resistant to the dramatic environmental changes that occur during in vitro preservation. Spermidine has various physiological functions including the anti‐oxidative effect. The main objective of this study was to clarify whether spermidine could protect boar sperm from the attack of reactive oxygen species under cryopreservation treatment. We set the concentrations of spermidine at 0, 2, 4, 6, and 8 mmol/L and evaluated the effects of spermidine on sperm motility, viability, malformation rates, kinetic parameters, membrane integrity, mitochondrial activity, DNA integrity, H2O2 content, malondialdehyde content, total antioxidant capacity, and antioxidant enzyme activity. Finally, the effects of spermidine on the sperm fertility were assessed by artificial insemination. The results showed that spermidine improved various physiological parameters of sperm in a dose‐dependent manner. The quality and antioxidant capacity of sperm cryopreserved with 6 mmol/L spermidine were significantly less reduced (P < 0.05), and the contents of malformation rate, H2O2, and malondialdehyde content were significantly decreased (P < 0.05). The significant increase in the number of litters indicated the possibility that spermidine had important practical value in pig reproduction (P < 0.05). Therefore, the addition of appropriate concentrations of spermidine to cryopreservation extenders may effectively improve the quality of boar sperm for in vitro preservation.

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Boar semen cryopreservation: State of the art, and international trade vision

Bolarin,

Berndtson,

Tejerina

et al. 2024

Animal Reproduction Science

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Effects of mitoquinone (MitoQ) supplementation during boar semen cryopreservation on sperm quality, antioxidant status and mitochondrial proteomics

Cited by 3 publications

References 52 publications

Metabolomics Analysis of Sodium Salicylate Improving the Preservation Quality of Ram Sperm

Metabolomics Analysis of Sodium Salicylate Improving the Preservation Quality of Ram Sperm

Exogenous spermidine effectively improves the quality of cryopreserved boar sperm

Boar semen cryopreservation: State of the art, and international trade vision

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