Comparison of the Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes for Confirmation of Stilbenes in Bovine Urine Samples Using LC–MS/MS QTRAP® System

Matraszek-Żuchowska, Iwona; Woźniak, Barbara; Posyniak, Andrzej

doi:10.1007/s10337-016-3121-1

Cited by 20 publications

(15 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As to MRM mode, the first quadrupole (Q1) also works filtering a specific precursor ion of interest (i); the second quadrupole (Q2) acts as collision cell inducing a characteristic product ion (ii); and the third quadrupole (Q3) filters specific m/z product ions (iii)—only analytes with this precursor/product ion combination will be detected. By working as a double mass filter, triple quadrupole drastically reduces noise and increases selectivity by providing complete structural specificity of the analyte 378,379 …”

Section: Resultsmentioning

confidence: 99%

“…By working as a double mass filter, triple quadrupole drastically reduces noise and increases selectivity by providing complete structural specificity of the analyte. 378,379 Despite the advantages of MRM, the dwell time of MRM transitions and the limited number of monitored ions throughout each chromatographic peak remained for several years as a problem of conventional MRM, implying longer chromatographic runs. 20 To overcome these limitations, some methods explored the use of a scheduled MRM algorithm, in which each MRM transition is monitored around its typical retention time and consequently more transitions might be monitored sequentially without losing sensitivity.…”

Section: Gc and Lc Separation Techniques Coupled To Msmentioning

confidence: 99%

See 1 more Smart Citation

Doping detection in animals: A review of analytical methodologies published from 1990 to 2019

Moreira

Carmo

Pinho

et al. 2021

Drug Testing and Analysis

View full text Add to dashboard Cite

Despite the impressive innate physical abilities of horses, camels, greyhounds, or pigeons, doping agents might be administered to these animals to improve their performance. To control these illegal practices, anti‐doping analytical methodologies have been developed. This review compiles the analytical methods that have been published for the detection of prohibited substances administered to animals involved in sports over 30 years. Relevant papers meeting the search criteria that discussed analytical methods aiming to detect and/or quantify doping substances in animal biological matrices published from 1990 to 2019 were considered. A total of 317 studies were included, of which 298 were related to horses, demonstrating significant advances toward the development of doping detection methods for equine sports. However, analytical methods for the detection of doping agents in sports involving other species are lacking. Due to enhanced accuracy and specificity, chromatographic analysis coupled to mass spectrometry detection is preferred over immunoassays. Regarding biological matrices, blood and urine remain the first choice, although alternative biological matrices, such as hair and feces, have been considered. With the increasing number and type of drugs used as doping agents, the analytes addressed in the published papers are diverse. It is very important to continue to detect and quantify these drugs, recognizing those that are most frequently used, in order to punish the abusers, protect animals' health, and ensure a healthier and genuine competition.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Gc and Lc Separation Techniques Coupled To Msmentioning

confidence: 99%

Doping detection in animals: A review of analytical methodologies published from 1990 to 2019

Moreira

Carmo

Pinho

et al. 2021

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…This accumulation signifies a 500-times increase in the scanning sensitivity and brings a high intensity of the mass spectra recorded in EPI mode. It double checks the doubtful samples by comparing the acquired mass spectra with the mass spectral library built from standards [35]. This study developed a new analytical strategy to combine QuEChERS sample preparation and QTRAP LC-MS/MS system to improve the efficiency of quantification and confirmation of carbamate residues while ensuring high degree of reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System

et al. 2018

View full text Add to dashboard Cite

In this research, fifteen carbamate pesticide residues were systematically analyzed by ultra-high performance liquid chromatography–quadrupole-linear ion trap mass spectrometry on a QTRAP 5500 system in both multiple reaction monitoring (MRM) and enhanced product ion (EPI) scan modes. The carbamate pesticide residues were extracted from a variety of samples by QuEChERS method and separated by a popular reverse phase column (Waters BEH C18). Except for the current conformation criteria including selected ion pairs, retention time and relative intensities from MRM scan mode, the presence of carbamate pesticide residues in diverse samples, especially some doubtful cases, could also be confirmed by the matching of carbamate pesticide spectra via EPI scan mode. Moreover, the fragmentation routes of fifteen carbamates were firstly explained based on the mass spectra obtained by a QTRAP system; the characteristic fragment ion from a neutral loss of CH3NCO (−57 Da) could be observed. The limits of detection and quantification for fifteen carbamates were 0.2–2.0 μg kg−1 and 0.5–5.0 μg kg−1, respectively. For the intra- (n = 3) and inter-day (n = 15) precisions, the recoveries of fifteen carbamates from spiked samples ranged from 88.1% to 118.4%, and the coefficients of variation (CVs) were all below 10%. The method was applied to pesticide residues detection in fruit, vegetable and green tea samples taken from local markets, in which carbamates were extensively detected but all below the standard of maximum residue limit.

show abstract

“…Lipids were extracted from the cortical tissues using Bligh and Dyer method (Bligh & Dyer, 1959), and extracted lipids were directly loaded onto the mass spectrometer (SCIENX QTRAP LC-MS/MS system; Framingham, MA) for targeted lipidomics. The phospholipid class was selectively targeted using characteristic scans, and the same mass spectrometer was operated in multi reaction monitoring (MRM) mode to detect specific species of very long chain fatty acids that are presumably synthesized by ELOVL4 (Matraszek-Zuchowska et al, 2016). Detected fatty acids are converted to relative percentages by dividing the peak intensity of each fatty acid species by the total peak intensities detected per animal.…”

Section: Lipidomics Of Phospholipid Fatty Acidsmentioning

confidence: 99%

Fatty acid metabolism changes in association with neurobehavioral deficits in animal models of fetal alcohol spectrum disorders

Hwang

Imamura

Bari³

et al. 2022

Preprint

View full text Add to dashboard Cite

Fetal alcohol spectrum disorders (FASD) show various behavioral problems due to prenatal alcohol exposure (PAE). Our previous study found significant changes in gene expressions linked to fatty acid metabolism in the brain of the PAE mouse model. Given the importance of fatty acids in normal brain functions and the contributions to neurodegenerative diseases, we hypothesized that the fatty acids changed by PAE contribute to neurobehavioral deficits in FASD. This study found an increase of palmitic acid and arachidonic acid in phospholipid compositions in the cerebral cortex of PAE at postnatal day 30. The increase of palmitic acid was consistent with the increase of the producing enzyme, fatty acid synthase (Fasn). The decrease of 26:6 fatty acid was also found in phospholipid. It is consistent with the increase of the Elongation of very long chain fatty acids protein 4 (ELOVL4) which uses 26:6 as a substrate for making very long chain fatty acids. However, there was no increase in the elongated products. Rather, we found an accumulation of the lipid droplets (LDs) in the PAE brain, suggesting changes in fatty acid metabolism that lead to the accumulation of excessive fatty acids. Although metabolic measurements, including plasma triglyceride level, were not affected by PAE, the abundance of fatty acid-related gut microbiota was altered. Interestingly, multi-omics association analysis revealed a potential contribution of the altered gut microbiota, primarily Ruminococcaceae that produces short chain fatty acid, to LD formation in the PAE brain and the behavioral problems, suggesting that the gut microbiome could serve as a tool to facilitate uncovering the brain pathophysiology of FASD and a potential target to mitigate neurobehavioral problems.

show abstract

Comparison of the Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes for Confirmation of Stilbenes in Bovine Urine Samples Using LC–MS/MS QTRAP® System

Cited by 20 publications

References 22 publications

Doping detection in animals: A review of analytical methodologies published from 1990 to 2019

Doping detection in animals: A review of analytical methodologies published from 1990 to 2019

Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System

Fatty acid metabolism changes in association with neurobehavioral deficits in animal models of fetal alcohol spectrum disorders

Contact Info

Product

Resources

About