Comparison of the methods of disinfection/sterilization of extracted human roots for research purposes

Pelozo, Laís Lima; Silva-Neto, Reinaldo Dias; Oliveira, Letícia de; Salvador, Sérgio Luiz de Souza; Corona, Silmara Aparecida Milori; Souza‐Gabriel, Aline Evangelista

doi:10.17219/dmp/144762

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…All prepared teeth were immersed in brain heart infusion (BHI) broth (Oxoid Ltd., Basingstoke, Hants, UK) and ultrasonicated for 1 min before autoclave sterilisation for 15 min at 121 °C [ 18 ]. Two samples were randomly selected and transferred into a sterile polystyrene bijou tube (Thermo Scientific TM Sterilin TM , ThermoFisher Scientific Inc., Waltham, MA, USA) containing 2 mL BHI for anaerobic incubation for 24 h to check for the effectiveness of the sterilisation process.…”

Section: Methodsmentioning

confidence: 99%

Effect of Passive Ultrasonic Irrigation, Er,Cr:YSGG Laser, and Photon-Induced Photoacoustic Streaming against Enterococcus faecalis Biofilms in the Apical Third of Root Canals

et al. 2023

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Purpose: This study aimed to compare the antibacterial effectiveness of passive ultrasonic irrigation (PUI), Er,Cr:YSGG laser (WTL), and photon-induced photoacoustic streaming (PIPS) using an Er:YAG laser against Enterococcus faecalis biofilms in the apical third of root canals. Methods: Root canals of 70 single-rooted human teeth were instrumented and infected with E. faecalis for 3 weeks to form biofilms. The samples were randomly divided into five groups as follows: (i) PUI + 3% NaOCl (n = 16); (ii) Er,Cr:YSGG laser (n = 16); (iii) PIPS + 3% NaOCl (n = 16); (iv) positive control group (n = 10); and (v) negative control group (n = 10). The bacterial content in the root canal was sampled using (a) the paper-point sampling method before (S1) and after (S2) treatment and (b) pulverising the apical 5 mm of the root. The number of bacteria recovered from each group was counted as colony-forming units (CFUs). The amount of reduction between the groups was compared with the Kruskal–Wallis test and post-test Dunn’s multiple comparisons tests. The significance level was set at 5% (p < 0.05). Results: The samples from the paper-point sampling method showed that the amount of bacteria before (S1) and after treatment (S2) was significantly different between PIPS and WTL, as well as between the PUI and WTL groups. In contrast, no significant difference was found between the PIPS and PUI groups. From the pulverised samples, the results indicated no significant difference among all experimental groups in the amount of bacterial reduction in the apical 5 mm of the root. Conclusions: PUI and PIPS showed a significantly greater reduction in bacterial content within the main root canal compared with the WTL. There was no difference among all experimental groups in the apical third of the root.

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Section: Methodsmentioning

confidence: 99%

Effect of Passive Ultrasonic Irrigation, Er,Cr:YSGG Laser, and Photon-Induced Photoacoustic Streaming against Enterococcus faecalis Biofilms in the Apical Third of Root Canals

et al. 2023

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Efficacy of an impression disinfectant solution after repeated use: An in vitro study

Chukwu,

Munn,

Wilson

et al. 2024

Heliyon

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Application of discipline integration in the practical teaching of endodontic‒restorative sequential treatment

Chen

Wang

Zhang

2023

Preprint

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Background: The cultivation of clinical reasoning and operative skills is the core of the practice course for undergraduates majoring in stomatology. To achieve this goal, a “discipline integration teaching method” is adopted in the practice course for root canal and prosthetic treatments in stomatology. Methods: A total of 893 students of West China School of Stomatology, Sichuan University, participated in this study. Of these students, 443 in the 2016-grade and 2017-grade groups used the traditional teaching method (Group TT) for preclinical practice courses (endodontics and prosthodontics training courses were taught separately), and 450 in the 2018-grade and 2019-grade groups used the discipline integration teaching method (Group DIT) for a preclinical practice course (root canal and prosthetic treatments were integrated into an endodontic‒restorative sequential treatment). The scores of the students (clinical skills examination and treatment planning) were compared between the two groups. In addition, students in Group DIT and their teachers were asked to complete a questionnaire on their attitudes and opinions about the DIT method in the preclinical course. Results: The results indicated that the DIT method was more effective than the TT method in enhancing students’ clinical reasoning and operational abilities. The questionnaire results showed that the teachers and students had recognized the DIT method. Conclusions: The DIT method significantly improved students’ learning quality and proved effective in the endodontic‒restorative sequential treatment practice course. This teaching method is expected to be further applied to other correlated subjects.

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Comparison of the methods of disinfection/sterilization of extracted human roots for research purposes

Cited by 3 publications

References 25 publications

Effect of Passive Ultrasonic Irrigation, Er,Cr:YSGG Laser, and Photon-Induced Photoacoustic Streaming against Enterococcus faecalis Biofilms in the Apical Third of Root Canals

Effect of Passive Ultrasonic Irrigation, Er,Cr:YSGG Laser, and Photon-Induced Photoacoustic Streaming against Enterococcus faecalis Biofilms in the Apical Third of Root Canals

Efficacy of an impression disinfectant solution after repeated use: An in vitro study

Application of discipline integration in the practical teaching of endodontic‒restorative sequential treatment

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