Comparison of the lipid composition of<i>Culex quinquefasciatus</i> and<i>Culex tritaeniorhynchus</i> cells obtained from the logarithmic and stationary phases of growth

McMeans, E.; Yang, Tao; Anderson, L. E.; Louloudes, Spiro J.; Jenkin, Howard M.

doi:10.1007/bf02532581

Cited by 2 publications

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Phospholipid composition ofCulex quinquefasciatus andCulex tritaeniorhynchus cells in logarithmic and stationary growth phases

Jenkin

McMeans

Anderson

et al. 1976

Lipids

View full text Add to dashboard Cite

Culex quinquefasciatus and Culex tritaeniorhynchus cells were grown in spinner culture and harvested in logarithmic and stationary phases of growth. The phospholipids were extracted from the cells, and the fatty acid profiles of the phospholipid classes were determined and compared. The major components were phosphatidylcholine and phosphatidylethanolamine, constituting greater than or equal to 80% of the phospholipid. The fatty acid profiles of lysophosphatidylcholine, phosphatidylinositol, and cardiolipin showed changes with aging of the Culex cells and between the species. In the lysophosphatidylcholine fraction, there was an increase in saturation of the fatty acids of the C. quinquefasciatus cells, and chain lengthening occurred in both species from the logarithmic to stationary phases of growth. In the phosphatidylinositiol fraction, both Culex species showed a decrease in monoenes and an increase in polyenes, while only the C. tritaeniorhynchus cells showed an increase in fatty acid chain length with aging. The C. quinquefasciatus cells had an increase in polyenes with aging in the cardiolipin fraction. Differences in the percentage composition of the fatty acids were shown in all the phospholipid fractions between the Culex species in the logarithmic phase of growth and all except the phosphatidylinositiol and cardiolipin fractions in the stationary phase.

show abstract