Mosquito Cell Culture

Kurtti, Timothy J.; Munderloh, Ulrike G.

doi:10.1016/b978-0-12-007903-2.50013-1

Cited by 12 publications

(7 citation statements)

References 135 publications

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“…Since the first mosquito cell line was established by Grace (1966), numerous cell lines have been established from various species of mosquitoes (Kurtti and Munderloh 1984). However, to date there has been only 1 derived from Ae.…”

mentioning

confidence: 99%

Establishment and Characterization of Three Cell Lines from Aedes triseriatus (Diptera: Culicidae)

Charpentier¹,

Belloncik²,

Ducros³

et al. 1995

Journal of Medical Entomology

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Three cell lines (A.t. GRIP-1, 2, and 3) were established from Aedes triseriatus (Say) embryonated eggs or neonate larvae and their morphology, growth, karyotype, and isozyme pattern were studied. The isozyme alleles observed in the 3 cell lines also were found in adults of the original mosquito colony. Each cell line differed in enzymatic, morphological, and karyotypical patterns. La Crosse encephalitis (LAC) and snowshoe hare (SSH) viruses, members of the California encephalitis virus group, were able to replicate in these 3 cell lines. Furthermore, these cell lines, especially A.t. GRIP-1, were more sensitive than the Aedes aegypti (L.) (ATC 10) cell line for detection of small amounts of delta-endotoxin of Bacillus thuringiensis serovar. israelensis (de Barjac).

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mentioning

confidence: 99%

Establishment and Characterization of Three Cell Lines from Aedes triseriatus (Diptera: Culicidae)

Charpentier¹,

Belloncik²,

Ducros³

et al. 1995

Journal of Medical Entomology

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“…Since the first cell line was established by Grace (1966), many mosquito cell lines have been established, the greatest number being in the Culicinae subfamily (Kurtti & Munderloh 1994). However, there were no prior records of cell lines of the Psorophora genus.…”

mentioning

confidence: 99%

“…baculovirus) to develop biological control strategies (Gelernter & Federici 1986). In the last few years, the use of molecular techniques to study cellular and viral gene in vitro has again stimulated interest in insect cell lines for physiological, biochemical and genetic research (Kurtti & Munderloh 1994, Shotkoski & Fallon 1994, Leake 1997.…”

mentioning

confidence: 99%

A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses

Bello

Rodrı́guez

Escovar

et al. 2001

Mem. Inst. Oswaldo Cruz

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“…Given that Aedes albopictus hosts a natural infection, one might wonder why Wolbachia is absent from older Ae. albopictus lines dating back to the late 1960s, before Wolbachia was known to be widespread in insects [63]. The possibility exists that early in their history, such lines may have been infected, followed by loss of Wolbachia over decades of use in multiple laboratories.…”

Section: Cell Lines Derived From Infected Insectsmentioning

confidence: 99%

Growth and Maintenance of Wolbachia in Insect Cell Lines

Fallon

2021

Insects

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The obligate intracellular microbe, Wolbachia pipientis (Rickettsiales; Anaplasmataceae), is a Gram-negative member of the alpha proteobacteria that infects arthropods and filarial worms. Although closely related to the genera Anaplasma and Ehrlichia, which include pathogens of humans, Wolbachia is uniquely associated with invertebrate hosts in the clade Ecdysozoa. Originally described in Culex pipiens mosquitoes, Wolbachia is currently represented by 17 supergroups and is believed to occur in half of all insect species. In mosquitoes, Wolbachia acts as a gene drive agent, with the potential to modify vector populations; in filarial worms, Wolbachia functions as a symbiont, and is a target for drug therapy. A small number of Wolbachia strains from supergroups A, B, and F have been maintained in insect cell lines, which are thought to provide a more permissive environment than the natural host. When transferred back to an insect host, Wolbachia produced in cultured cells are infectious and retain reproductive phenotypes. Here, I review applications of insect cell lines in Wolbachia research and describe conditions that facilitate Wolbachia infection and replication in naive host cells. Progress in manipulation of Wolbachia in vitro will enable genetic and biochemical advances that will facilitate eventual genetic engineering of this important biological control agent.

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Mosquito Cell Culture

Cited by 12 publications

References 135 publications

Establishment and Characterization of Three Cell Lines from Aedes triseriatus (Diptera: Culicidae)

Establishment and Characterization of Three Cell Lines from Aedes triseriatus (Diptera: Culicidae)

A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses

Growth and Maintenance of Wolbachia in Insect Cell Lines

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