Comparison of the Kato-Katz technique, hatching test and indirect hemagglutination assay (IHA) for the diagnosis of Schistosoma japonicum infection in China

Yu, Jingyi; Vlas, Sake J. de; Jiang, Qingwu; Gryseels, B.

doi:10.1016/j.parint.2006.11.002

Cited by 137 publications

(129 citation statements)

References 22 publications

Supporting

Mentioning

127

Contrasting

Order By: Relevance

“…The indirect hemagglutination assay blood test for antibodies to S. japonicum was performed on all consenting individuals between ages 3 and 65 y in each study village. Stool samples obtained from seropositive humans were tested for S. japonicum infection using the miracidia hatching method and Kato-Katz smears (23). Fecal samples were also collected from cattle, water buffalo, goats, pigs, dogs, cats, and rodents and tested for miracidia hatching as described in ref.…”

Section: Methodsmentioning

confidence: 99%

Identifying host species driving transmission of schistosomiasis japonica, a multihost parasite system, in China

Rudge

Webster

Lü

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

116

View full text Add to dashboard Cite

Understanding disease transmission dynamics in multihost parasite systems is a research priority for control and potential elimination of many infectious diseases. In China, despite decades of multifaceted control efforts against schistosomiasis, the indirectly transmitted helminth Schistosoma japonicum remains endemic, partly because of the presence of zoonotic reservoirs. We used mathematical modeling and conceptual frameworks of multihost transmission ecology to assess the relative importance of various definitive host species for S. japonicum transmission in contrasting hilly and marshland areas of China. We examine whether directing control interventions against zoonotic reservoirs could further reduce incidence of infection in humans or even eliminate transmission. Results suggest that, under current control programs, infections in humans result from spillover of transmission among zoonotic reservoirs. Estimates of the basic reproduction number within each species suggest that bovines (water buffalo and cattle) maintained transmission in the marshland area and that the recent removal of bovines from this area could achieve local elimination of transmission. However, the sole use of antifecundity S. japonicum vaccines for bovines, at least at current efficacies, may not achieve elimination in areas of comparable endemicity where removal of bovines is not a feasible option. The results also suggest that rodents drive transmission in the hilly area. Therefore, although targeting bovines could further reduce and potentially interrupt transmission in marshland regions of China, elimination of S. japonicum could prove more challenging in areas where rodents might maintain transmission. In conclusion, we show how mathematical modeling can give important insights into multihost transmission of indirectly transmitted pathogens.

show abstract

Section: Methodsmentioning

confidence: 99%

Identifying host species driving transmission of schistosomiasis japonica, a multihost parasite system, in China

Rudge

Webster

Lü

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

116

View full text Add to dashboard Cite

show abstract

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…Moreover, currently, the diagnosis of schistosomiasis relies primarily on the direct microscopic detection of parasite ova, generally using the KK procedure, or by serology (Cai et al, 2014;WHO, 2013;Yu et al, 2007). While the KK is cheap and easy to implement, it can only detect patent infections and has relatively low sensitivity, particularly with light infections that occur in low disease transmission areas (Berhe et al, 2004;Kongs et al, 2001;Yu et al, 2007). In the case of S. japonicum, oviposition occurs 4-6 weeks post-infection and egg detection by microscopy can lack sensitivity due to variability in egg release and sampling related issues such as clumping of eggs and limited egg excretion in low intensity infections (Berhe et al, 2004;Kongs et al, 2001;Yu et al, 2007).…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…While the KK is cheap and easy to implement, it can only detect patent infections and has relatively low sensitivity, particularly with light infections that occur in low disease transmission areas (Berhe et al, 2004;Kongs et al, 2001;Yu et al, 2007). In the case of S. japonicum, oviposition occurs 4-6 weeks post-infection and egg detection by microscopy can lack sensitivity due to variability in egg release and sampling related issues such as clumping of eggs and limited egg excretion in low intensity infections (Berhe et al, 2004;Kongs et al, 2001;Yu et al, 2007). Serological techniques have low specificity and high cross reactivity, and cannot differentiate between an active and a past infection (Doenhoff et al, 2004;Xie et al, 2014).…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…Consequently, it is important to have available sensitive diagnostic tools that can detect low prevalence and low intensity infections, particularly within the framework of population surveillance and the monitoring of schistosomiasis control programs. Conventional parasitological diagnostic methods, such as the Kato-Katz (KK) thick smear examination or the miracidial hatching test (MHT), lack the requisite sensitivity in areas with low schistosome infection rates (Kongs et al, 2001;Spear et al, 2011;Yu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimisation of a droplet digital PCR assay for the diagnosis of Schistosoma japonicum infection: A duplex approach with DNA binding dye chemistry

Weerakoon

Gordon

Gobert

et al. 2016

Journal of Microbiological Methods

View full text Add to dashboard Cite

Schistosomiasis is a chronically debilitating helminth infection with a significant socioeconomic and public health impact. Accurate diagnostics play a pivotal role in achieving current schistosomiasis control and elimination goals. However, many of the current diagnostics procedures, which rely on detection of schistosome eggs, have major limitations including lack of accuracy and the inability to detect pre-patent infections. DNA-based detection methods provide a viable alternative to the current tests commonly used for schistosomiasis diagnosis. Here we describe the optimisation of a novel droplet digital PCR (ddPCR) duplex assay for the diagnosis of Schistosoma japonicum which provides improved detection sensitivity and specificity. The assay involves the amplification of two specific and abundant target gene sequences in S. japonicum; a retrotransposon (SjR2) and a portion of a mitochondrial gene (nad1). The assay detected target sequences in different sources of schistosome DNA isolated from adult worms, schistosomules and eggs, and exhibits a high level of specificity, thereby representing an ideal tool for the detection of low levels of parasite DNA in different clinical samples including parasite cell free DNA in the host circulation and other bodily fluids. Moreover, being quantitative, the assay can be used to determine parasite infection intensity and, could provide an important tool for the detection of low intensity infections in low prevalence schistosomiasis-endemic areas.

show abstract

Therapeutic and prophylactic drug interventions for Schistosomiasis japonicum

Alejandria

Roman

Gonzales

et al. 2012

Cochrane Database of Systematic Reviews

View full text Add to dashboard Cite

Comparison of the Kato-Katz technique, hatching test and indirect hemagglutination assay (IHA) for the diagnosis of Schistosoma japonicum infection in China

Cited by 137 publications

References 22 publications

Identifying host species driving transmission of schistosomiasis japonica, a multihost parasite system, in China

Identifying host species driving transmission of schistosomiasis japonica, a multihost parasite system, in China

Optimisation of a droplet digital PCR assay for the diagnosis of Schistosoma japonicum infection: A duplex approach with DNA binding dye chemistry

Therapeutic and prophylactic drug interventions for Schistosomiasis japonicum

Contact Info

Product

Resources

About