Comparison of the Intestinal Bacteria in Specific Pathogen Free Mice from Different Breeders

Hirayama, Kazuhiro; Endo, Kimiko; Kawamura, Seiji; Mitsuoka, Tomotari

doi:10.1538/expanim1978.39.2_263

“…Previous studies using culture methods and histological examination reported that many unclassified bacteria and strictly anaerobic bacteria inhabit the cecum of SPF and normal mice [2,9]. Furthermore, it has been reported that microbiota can be significantly altered by diets, different breeders and other factors [33]. Because variation of intestinal microbiota in experimental animals can influence experimental results, standardization of intestinal microbiota is required.…”

Section: Comparison Between T-rflp Analysis and 16s Rrna Gene Clone Lmentioning

confidence: 99%

Maturation of the murine cecal microbiota as revealed by terminal restriction fragment length polymorphism and 16S rRNA gene clone libraries

Kibe

¹

2004

FEMS Microbiology Letters

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The maturation of murine cecal microbiota was determined by terminal restriction fragment polymorphism (T-RFLP) and 16S rRNA gene clone libraries. Cecal microbiota in specific pathogen free (SPF) mice aged four to 10 weeks were collected. The cluster of samples in 4-week-old mice was different from those of other ages based on T-RFLP profiles. The majority of clones obtained in this study belonged to the Clostridium coccoides (C. coccoides) group, the Bacteroides group or the Lactobacillus group. Phylogenetic analysis showed characteristic clusters composed of new operational taxonomic unit (OTU) of the C. coccoides and Bacteroides groups. The existence of a large number of yet unidentified bacteria inhabiting the murine cecum was demonstrated by 16S rRNA gene clone libraries. T-RFLP analysis data were more complex and more sensitive than the patterns generated by computer simulation of 16S rRNA gene clone library analysis data. T-RFLP revealed development with maturation of cecal microbiota including unidentified bacteria of SPF mice.

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“…Previous studies using culture methods and histological examination reported that many unclassified bacteria and strictly anaerobic bacteria inhabit the cecum of SPF and normal mice [2,9]. Furthermore, it has been reported that microbiota can be significantly altered by diets, different breeders and other factors [33]. Because variation of intestinal microbiota in experimental animals can influence experimental results, standardization of intestinal microbiota is required.…”

Section: Resultsmentioning

confidence: 99%

Maturation of the murine cecal microbiota as revealed by terminal restriction fragment length polymorphism and 16S rRNA gene clone libraries

Kibe

¹

,

Sakamoto

²

,

Hayashi

³

et al. 2004

FEMS Microbiology Letters

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The maturation of murine cecal microbiota was determined by terminal restriction fragment polymorphism (T-RFLP) and 16S rRNA gene clone libraries. Cecal microbiota in specific pathogen free (SPF) mice aged four to 10 weeks were collected. The cluster of samples in 4-week-old mice was different from those of other ages based on T-RFLP profiles. The majority of clones obtained in this study belonged to the Clostridium coccoides (C. coccoides) group, the Bacteroides group or the Lactobacillus group. Phylogenetic analysis showed characteristic clusters composed of new operational taxonomic unit (OTU) of the C. coccoides and Bacteroides groups. The existence of a large number of yet unidentified bacteria inhabiting the murine cecum was demonstrated by 16S rRNA gene clone libraries. T-RFLP analysis data were more complex and more sensitive than the patterns generated by computer simulation of 16S rRNA gene clone library analysis data. T-RFLP revealed development with maturation of cecal microbiota including unidentified bacteria of SPF mice.

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“…Our results agree with studies in which differences in bacterial populations, other than lactobacilli, between animals from different suppliers were reported (O’Rourke et al . 1988; Hirayama et al . 1990).…”

Section: Discussionmentioning

confidence: 99%

Comparison of faecal Lactobacillus populations in experimental animals from different breeding facilities and possible consequences for probiotic studies

Waard

¹

,

Snel

²

,

Bokken

³

et al. 2002

Lett Appl Microbiol

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Aims: The effect of probiotic lactobacilli is likely dependent on the indigenous Lactobacillus strains in the intestinal tract. Since a substantial number of probiotic studies is performed in rodents, we compared the Lactobacillus strains of different rat and mouse populations in three animal facilities. Methods and Results: SDS-PAGE and 16S rDNA analysis of cultured faecal lactobacilli revealed that different Lactobacillus strains were detected in genetically similar Wistar rats bred at different locations. Further, within the same animal facility host genetics did not affect the types of the predominant lactobacilli strains. Conclusions: Our results show that the environmental background of laboratory animals rather than host genetics determines the indigenous Lactobacillus strains that are found. Significance and Impact of the Study: Our findings underline the importance of microflora analysis in probiotic studies.

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Comparison of the Intestinal Bacteria in Specific Pathogen Free Mice from Different Breeders

Cited by 21 publications

References 9 publications

Maturation of the murine cecal microbiota as revealed by terminal restriction fragment length polymorphism and 16S rRNA gene clone libraries

Maturation of the murine cecal microbiota as revealed by terminal restriction fragment length polymorphism and 16S rRNA gene clone libraries

Maturation of the murine cecal microbiota as revealed by terminal restriction fragment length polymorphism and 16S rRNA gene clone libraries

Comparison of faecal Lactobacillus populations in experimental animals from different breeding facilities and possible consequences for probiotic studies

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