Comparison of faecal Lactobacillus populations in experimental animals from different breeding facilities and possible consequences for probiotic studies

Waard, Rick de; Snel, J.; Bokken, G.C.A.M.; Tan, Paris S. T.; Schut, Frits; Veld, J.H.J. Huis in‘t

doi:10.1046/j.1472-765x.2002.01051.x

Cited by 14 publications

(13 citation statements)

References 17 publications

(23 reference statements)

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“…Thus, the interindividual differences in intestinal repertoire could reflect differences in current antigenic exposure between rats or reflect the end result of sequential accumulation of small differences in the level of antigen exposure over time. Such differences are likely to be small at any one time‐point: studies of intestinal flora suggest some effect of genetics, but a strong effect of environment, such that animals from the same source had very similar flora 25,26 …”

Section: Discussionmentioning

confidence: 99%

Antigen receptor V‐segment usage in mucosal T cells

et al. 2007

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Summary In the accepted model of lymphocyte intestinal homing, naïve T cells recirculate via organized lymphoid tissues, whilst induced effector/memory cells home to the intestinal mucosa. In order to assess the T‐cell‐receptor repertoire in the intestine and gut‐associated lymphoid tissue (GALT), spectratyping was performed on the proximal and the distal intestine, spleen and mesenteric lymph node tissue from six PVG rats. The products were analysed with an automated sequencer and statistical analyses were performed with hierarchical cluster analysis. This demonstrated the presence of a restricted T‐cell repertoire in the small intestine compared with that in the mesenteric lymph nodes and the spleen. It also demonstrated marked differences in repertoire between individual, fully inbred rats maintained under apparently identical conditions in the same cage and fed identical diets. In addition, this work demonstrated marked differences between repertoires in the proximal and the distal intestine. Such marked differences are likely to reflect the end result of increasing divergence over time produced by relatively subtle effects of environment and antigenic load. Equally, marked differences in repertoire between small intestinal segments within individual rats indicate selective recruitment or retention of specific clones, presumably antigen‐driven.

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Section: Discussionmentioning

confidence: 99%

Antigen receptor V‐segment usage in mucosal T cells

et al. 2007

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“…Differences in composition of the Lactobacillus flora might also be due to genetic factors (Tannock et al ., 1994), age and intestinal location (Lu et al ., 2003), ecological factors such as diet and lifestyle (Tannock et al ., 1994) and even the type of housing (De Waard et al ., 2002).…”

Section: Discussionmentioning

confidence: 99%

CloacalLactobacillusisolates from broilers show high prevalence of resistance towards macrolide and lincosamide antibiotics

et al. 2006

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Eighty-seven Lactobacillus strains isolated from cloacal swabs of broiler chickens derived from 20 different farms in Belgium were identified to species level and tested for susceptibility to macrolide and lincosamide antibiotics. Five different Lactobacillus species were identified as being predominantly present in the cloacae of broilers: Lactobacillus crispatus, Lactobacillus salivarius subsp. salivarius, Lactobacillus amylovorus, Lactobacillus gallinarum and Lactobacillu sreuteri. Acquired resistance prevalence to macrolides and lincosamides was very high in the investigated lactobacilli: 89% of the strains were resistant to either or both lincosamide and macrolide class antibiotics. The vast majority of these resistant strains (96%) displayed constitutive resistance. More than one-half of the macrolide and/or lincosamide resistant strains carried an erm(B), erm(C), mef(A), lnu(A) gene or a combination of these genes.

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“…1983) from conventional and SPF mice were L. johnsonii . Recently, it was reported that L. johnsonii , L. murinus and L. reuteri were detected predominantly in faecal samples from SPF mice by 16S rDNA sequences analysis (De Waard et al. 2002).…”

Section: Discussionmentioning

confidence: 99%

Species-specific oligonucleotide probes for the detection and identification ofLactobacillusisolated from mouse faeces

Park

Itoh

2005

Journal of Applied Microbiology

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S . H . P A R K A N D K . I T O H . 2005.Aims: To develop species-specific monitoring techniques for rapid detection and identification of Lactobacillus isolated from mouse faeces. Methods and Results: The specificity of oligonucleotide probes was evaluated by dot blot hybridization to 16S rDNA and 23S rDNA amplified by PCR from 12 Lactobacillus type strains and 100 strains of Lactobacillus isolated from mouse faeces. Oligonucleotide probes specific for each Lactobacillus species hybridized only with targeted rDNA. The Lactobacillus strains isolated from mouse faeces were identified mainly as Lactobacillus intestinalis, L. johnsonii, L. murinus and L. reuteri using species-specific probes. 16S rDNA of eight unidentified isolates were sequenced and two new probes were designed. Four of eight strains of unhybridized Lactobacillus were identified as L. johnsonii/gasseri group, and the remaining four strains as L. vaginalis. Conclusions: The species-specific probe set of L. intestinalis, L. johnsonii, L. murinus, L. reuteri and L. vaginalis in this study was efficient for rapid identification of Lactobacillus isolated from mouse faeces. Significance and Impact of the Study: The oligonucleotide probe set for Lactobacillus species harboured in the mouse intestine, can be used for rapid identification of lactobacilli and monitoring of the faecal Lactobacillus community.

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Comparison of faecal Lactobacillus populations in experimental animals from different breeding facilities and possible consequences for probiotic studies

Cited by 14 publications

References 17 publications

Antigen receptor V‐segment usage in mucosal T cells

Antigen receptor V‐segment usage in mucosal T cells

CloacalLactobacillusisolates from broilers show high prevalence of resistance towards macrolide and lincosamide antibiotics

Species-specific oligonucleotide probes for the detection and identification ofLactobacillusisolated from mouse faeces

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