Comparison of the inhibition potential of parthenolide and micheliolide on various UDP-glucuronosyltransferase isoforms

Gao, Weifeng; Li, Yixuan; Zhang, Weihua; Tao, Ran; Yin, Tingting; Wang, Yijia; Liu, Lina; Fu, Zhiwei; Li, Sainan; Liu, Nai-Rong; Zhang, Heng; Liu, Guang; Zhao, Li-Zhong; Zhang, Xi-Peng; Zhang, Chun-Ze

doi:10.1080/00498254.2018.1544383

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…According to the chromatogram of 4-MU, a nonselective substrate of UGT2B7, with different concentration gradients (0-1 mM), the standard curve of 4-MU under 316 nm irradiation in C18 HPLC column (4.6 × 250 mm, 5 µm, GL Science, Fukushima, Japan) at a flow rate of 1 mL/min was obtained [30][31][32][33][34][35]. Rutin, nicotifiorin, isovitexin, vitexin, HPAA, DHPAA, aucubin, and asperuloside were dissolved in DMSO.…”

Section: Hplc Assessment Of Inhibitory Effects Of Flavones Against Hu...mentioning

confidence: 99%

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Properties of Dietary Flavone Glycosides, Aglycones, and Metabolites on the Catalysis of Human Endoplasmic Reticulum Uridine Diphosphate Glucuronosyltransferase 2B7 (UGT2B7)

Xu,

Lv,

Cui

et al. 2023

Nutrients

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Flavone glycosides, their aglycones, and metabolites are the major phytochemicals in dietary intake. However, there are still many unknowns about the cellular utilization and active sites of these natural products. Uridine diphosphate glucuronosyltransferases (UGTs) in the endoplasmic reticulum have gene polymorphism distribution in the population and widely mediate the absorption and metabolism of endogenous and exogenous compounds by catalyzing the covalent addition of glucuronic acid and various lipophilic chemicals. Firstly, we found that rutin, a typical flavone O-glycoside, has a stronger UGT2B7 binding effect than its metabolites. After testing a larger number of flavonoids with different aglycones, their aglycones, and metabolites, we demonstrated that typical dietary flavone O-glycosides generally have high binding affinities towards UGT2B7 protein, but the flavone C-glycosides and the phenolic acid metabolites of flavones had no significant effect on this. With the disposition of 4-methylumbelliferone examined by HPLC assay, we determined that 10 μM rutin and nicotifiorin could significantly inhibit the activity of recombinant UGT2B7 protein, which is stronger than isovitexin, vitexin, 3-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid. In addition, in vitro experiments showed that in normal and doxorubicin-induced lipid composition, both flavone O-glycosides rutin and flavone C-glycosides isovitexin at 10 μM had no significant effect on the expression of UGT1A1, UGT2B4, UGT2B7, and UGT2B15 genes for 24 h exposure. The obtained results enrich the regulatory properties of dietary flavone glycosides, aglycones, and metabolites towards the catalysis of UGTs and will contribute to the establishment of a precise nutritional intervention system based on lipid bilayers and theories of nutrients on endoplasmic reticulum and mitochondria communication.

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Section: Hplc Assessment Of Inhibitory Effects Of Flavones Against Hu...mentioning

confidence: 99%

“…4-methylumbelliferone (4-MU) has been widely used as a non-specific substrate of UGT to determine the effect of chemicals on UGT activity [30][31][32][33][34][35]. Catalyzed by UGT, 4-MU obtains the glucuronic acid group transferred from UDPGA through a glucuronidation binding reaction.…”

Section: Introductionmentioning

confidence: 99%