Transcriptome analysis reveals important candidate gene families related to oligosaccharides biosynthesis in Morinda officinalis

Liu, Mengyun; Li, Yang; Cai, Miaomiao; Feng, Chong; Zhao, Zhimin; Yang, Dongzi; Ding, Ping

doi:10.1016/j.plaphy.2021.09.028

Cited by 10 publications

(9 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GPI is a multifunctional cytoplasmic enzyme that converts D-fructose to UDP-glucose, which in turn undergoes several transformations ( Liu et al, 2021 ). Pgm belongs to the family of hexose phosphate metastases and is widespread in microorganisms, where its main function is to catalyze the interconversion of D-glucose 1-phosphate and D-glucose 6-phosphate and to keep them in dynamic balance ( Chauton et al, 2013 ).…”

Section: Resultsmentioning

confidence: 99%

Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments

Xie

Wang

Xie

et al. 2022

Current Research in Food Science

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments

Xie

Wang

Xie

et al. 2022

Current Research in Food Science

View full text Add to dashboard Cite

“…Ultrasonic extraction was performed for 20 min after half an hour of rest, and then centrifuged at 3000 rpm for 10 min. The upper layer was collected, filtered through at 0.22 μm microporous membrane filtration and then transferred for HPLC-ELSD analysis [ 71 ].…”

Section: Methodsmentioning

confidence: 99%

“…The concentration and size of the cDNA library were determined using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, US) and quantified with Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Furthermore, all libraries were sequenced using the Illumina HiSeq 4000 platform with a paired-end reads length of 150 bp at Science Corporation of Gene (Guangzhou, China) [ 71 ].…”

Section: Methodsmentioning

confidence: 99%

“…The mobile phase consisted of 0.2% triethylamine acetonitrile (A) -0.2% triethylamine water (B), and the linear gradient was set as follows: 0–10 min for 75% A to 70% A, 10–20 min for 70% A, 20–45 min for 70% A to 60% A, 45–60 min for 60% A, and 60–63 min for 60% A to 75% A. The following parameters were also set: volume flow of 0.8 mL·min − 1 , sample injection volume of 20 μL, column temperature of 35 °C, ELSD drift tube temperature of 75 °C, and nitrogen flow of 2.5 L ·min − 1 [ 71 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Phenotypic, chemical component and molecular assessment of genetic diversity and population structure of Morinda officinalis germplasm

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Morinda officinalis How (MO) is a perennial herb distributed in tropical and subtropical regions, which known as one of the “Four Southern Herbal Medicines”. The extent of genetic variability and the population structure of MO are presently little understood. Here, nine morphological traits, six chemical components and Single nucleotide polymorphism (SNP) markers were used in integrative research of MO germplasm variation among 88 individuals collected from ten populations across four geographical provinces of China. Results Both phenotype and chemical composition have significant genetic variation, and there is a certain correlation between them such as root diameter and the nystose content, as well as geographical distribution. The principal component analysis (PCA) showed the leaf length, leaf width, nystose, 1F-furanosaccharide nystose, and the section color were the major contributors to diversity. The cluster analysis based on phenotypic and oligosaccharide data distinguished three significant groups, which was consistent with the result of a corresponding analysis with 228,615 SNP markers, and importantly, they all showed a significant correlation with geographical origin. However, there was little similarity between two cluster results. The Shannon’s information index (I) varied from 0.17 to 0.53 with a mean of 0.37, suggesting a high level of genetic diversity in MO populations, which mainly existed among individuals within populations, accounting for 99.66% of the total according to the analysis of molecular variance (AMOVA) results. Each population also maintains the connection because of certain gene communication, so that the genetic differentiation between populations was not very significant. The STRUCTURE software was used to analyse the population structure and the result showed that 88 accessions were clustered into three groups, and 67% of them were pure type, which was also confirmed through PCA. Conclusions The comprehensive study of phenotypic, chemical and molecular markers will provide valuable information for future breeding plans and understanding the phylogenetic relationship of MO population.

show abstract

“…It is not clear that the differential expression level of genes in the whole genome of Monascus cultivated with different nutritional ingredients based on RB medium. RNA sequencing (RNA-seq) is a potent and effective way of discovering new genes on a large scale ( Liu et al, 2021 ). It has been widely used to characterize differentially expressed genes (DEGs) under various conditions ( Shin et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus

Chen,

2023

Front. Microbiol.

View full text Add to dashboard Cite

To reduce the production cost of Monascus pigments (MPs), the utilization of rice bran (RB), an agricultural waste product, as a substrate in submerged fermentation was conducted in this study. To improve MP production, different nutritional ingredients including mannitol (Man), NH4NO3 (AN), ZnSO4 (Zn), and optimization (Opti), which was a synthesis of the three above ones, were added in rice bran (RB) medium. The yields of MPs, pigment constituents, and growth and development of Monascus purpureus M9 were investigated in this study. Man had the maximum color value of 3,532 U/g, which was 18.69 times more than that of RB and reached up to 76.65% of the value of rice (Rice) fermentation. Man significantly increased the production of two orange pigments, monascorubrin and rubropunctatin, of which the yields were 69.49 and 95.36% of the counterpart of Rice. The biomass and colony diameter of Opti presented the maximum value among different groups. AN and RB induced more asexual spore formation, whereas Opti and Man promoted sexual spore production. Comparative transcriptomic analysis showed that different nutritional ingredients led to changes in pigment production, promoting the growth and development of M. purpureus M9 through the regulation of related gene expression. Man and Opti improved MP production by regulating the primary metabolism, including the Embden–Meyerhof pathway (EMP), the pentose phosphate (PP) pathway, the tricarboxylic (TCA) cycle, fatty acid degradation (FAD), fatty acid biosynthesis (FAB), amino acid metabolism (AAM), and fructose and mannose metabolism (FMM), to provide the precursors (acetyl-CoA and malonyl-CoA) for MP biosynthesis. This study presents a low-cost method for increasing MP production and explains the molecular mechanisms of different nutritional ingredients for enhancing MP biosynthesis.

show abstract

Transcriptome analysis reveals important candidate gene families related to oligosaccharides biosynthesis in Morinda officinalis

Cited by 10 publications

References 62 publications

Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments

Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments

Phenotypic, chemical component and molecular assessment of genetic diversity and population structure of Morinda officinalis germplasm

Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus

Contact Info

Product

Resources

About