Comparison of the in vitro Effect of Chemical and Herbal Mouthwashes on Candida albicans

Abstract

Background:During the recent decades research has focused to find scientific evidence for the effects of herbal medicines. Researchers are interested in herbal remedies for medication and aim to substitute herbal material instead of chemical formula with limited side effects for human being.Objectives:The aim of the current study was to compare the in vitro effect of herbal and chemical mouthwashes against Candida albicans.Materials and Methods:In this research, we used a standard strain of C. albicans, PTCC 5027. The suspension was made by a fresh culture of C. albicans (24 hours) and the optical density (turbidity equating to a McFarland standard of 0.5) was read at 530 nm. The C. albicans suspension was cultured on Sabouraud dextrose agar plate. Next, two wells were filled with mouthwashes and after incubation at 30ºC for 24 hours, the inhibition zone was measured. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of mouthwashes were determined. Data were analyzed using the SPSS software, independent T-tests and one-sided variance analysis (ANOVA-one way).Results:Based on these findings on agar diffusion with (P = 0.764), MIC and MFC tests (P = 0.879), there were no significant differences between the antifungal effect of herbal and chemical mouthwashes.Conclusions:This study showed that, chemical mouthwashes acted better than herbal mouthwashes and among different chemical mouthwashes, Oral B was most effective.

Chemical composition and in vitro activity of Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. essential oils towards oral isolates of Candida albicans and Candida glabrata

“…Today there are several classes of antifungal drugs available for the treatment of fungal infections in humans. In oral candidiases, various antimycotics have been used as antiseptic agents, with fluconazole, nystatin suspension and chlorhexidine commonly applied topically as mouthwash [21]. However, several studies have been performed to search for natural compounds with antifungal activity, especially among aromatic plants due to their high content of biologically active compounds, mainly EOs [5,6,14].…”

“…In turn, 23 and 25 components were found in summer savory (S. hortensis L.) and thyme oils (T. vulgaris L.), respectively. The least number of components (21), were identified in oregano oil (O. vulgare L.). The content of individual volatile components of the examined essential oils was presented as their percentage share among the detected compounds.…”

Antifungal activity of various essential oils against Saccharomyces cerevisiae depends on disruption of cell membrane integrity

“…Plates were incubated for 48 h at 25°C. After incubation, viability of yeasts was controlled based on turbidity clearance (Talebi et al, 2014).…”

Antifungal potential of Fenugreek Seeds (Trigonella foenum-graecum) Crude Extracts against Microsporum gypseum

“…Many antimicrobial agents are presently accessible for the treatment and management of infectious diseases (Karuppiah and Rajaram, 2012). In order to overcome the ill effects and resistance caused due to synthetic drugs, the World Health Organization have motivated many researchers to exploit natural products for their great therapeutic potential (Talebi et al, 2014). A huge variety of herbal antifungal agents derived from traditional medicinal plants are existing for the treatment of dermatophytoses (Kimm et al, 2015).…”