Comparison of the immobilization of lipase from Pseudomonas fluorescens on divinylsulfone or p-benzoquinone activated support

Rios, Nathália Saraiva; Neto, Davino M. Andrade; Santos, José Cleiton Sousa dos; Fechine, Pierre Basílio Almeida; Fernández‐Lafuente, Roberto; Gonçalves, Luciana Rocha Barros

doi:10.1016/j.ijbiomac.2019.05.106

Cited by 88 publications

(28 citation statements)

References 56 publications

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“…Despite its excellent performance potential, industrial applications were made impossible due to these undesirable characteristics [195,196]. In this scenario, immobilization techniques stand out as alternatives to overcome these limitations, since they can offer better stability, increased activity and selectivity, excellent resistance, improvements in product separation and purification, and the possibility of enzyme reuse, rendering processes increasingly efficient [192][193][194][195][196][197][198][199][200][201][202][203][204][205][206].…”

Section: Enzymesmentioning

confidence: 99%

“…Enzymatic immobilization is based on the confinement of enzyme molecules on the surface of a reliable support that is different from that in which the substrate or products are present [202][203][204][205][206][207][208][209]. In contrast with their solubilized form, immobilized enzymes provide a large enzyme to substrate ratio, efficient digestion, and secure handling, in addition to showing more significant activity and the possibility of reuse for several cycles [197,[210][211][212][213].…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

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Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

et al. 2021

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Among the many biological entities employed in the development of biosensors, enzymes have attracted the most attention. Nanotechnology has been fostering excellent prospects in the development of enzymatic biosensors, since enzyme immobilization onto conductive nanostructures can improve characteristics that are crucial in biosensor transduction, such as surface-to-volume ratio, signal response, selectivity, sensitivity, conductivity, and biocatalytic activity, among others. These and other advantages of nanomaterial-based enzymatic biosensors are discussed in this work via the compilation of several reports on their applications in different industrial segments. To provide detailed insights into the state of the art of this technology, all the relevant concepts around the topic are discussed, including the properties of enzymes, the mechanisms involved in their immobilization, and the application of different enzyme-derived biosensors and nanomaterials. Finally, there is a discussion around the pressing challenges in this technology, which will be useful for guiding the development of future research in the area.

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Section: Enzymesmentioning

confidence: 99%

Section: Enzyme Immobilizationmentioning

confidence: 99%

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

et al. 2021

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“…Epichlorohydrin and some di-epoxides may be used to activate supports with epoxy groups, this group may react with many different groups of a protein but at a low reaction rate, making them interesting but not ideal ones, although some heterofunctional supports have given good results [47][48][49]. Divinylsulfone has been recently recognized as a very suitable support activator, that permits very intense multipoint covalent attachment, but it is relatively toxic [50][51][52]. Glutaraldehyde is perhaps the most utilized reagent for enzyme immobilization, as it can react with primary amino groups located in a support and the resulting supports have low capability to react with the primary amino groups of a protein [53].…”

Section: Biocatalysis and Biocatalysts Designmentioning

confidence: 99%

Genipin as An Emergent Tool in the Design of Biocatalysts: Mechanism of Reaction and Applications

et al. 2019

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Genipin is a reagent isolated from the Gardenia jasminoides fruit extract, and whose low toxicity and good crosslinking properties have converted it into a reactive whose popularity is increasing by the day. These properties have made it widely used in many medical applications, mainly in the production of chitosan materials (crosslinked by this reactive), biological scaffolds for tissue engineering, and nanoparticles of chitosan and nanogels of proteins for controlled drug delivery, the genipin crosslinking being a key point to strengthen the stability of these materials. This review is focused on the mechanism of reaction of this reagent and its use in the design of biocatalysts, where genipin plays a double role, as a support activating agent and as inter- or intramolecular crosslinker. Its low toxicity makes this compound an ideal alterative to glutaraldehyde in these processes. Moreover, in some cases the features of the biocatalysts prepared using genipin surpassed those of the biocatalysts prepared using other standard crosslinkers, even disregarding toxicity. In this way, genipin is a very promising reagent in the design of biocatalysts.

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“…Meeting with the current public policies for green and sustainable development, the interest in using immobilized lipases in industrial processes results in increased funding for the studies thereof, causing new support materials and immobilization processes to be discovered and improved [60][61][62][63][64][65][66][67][68]. In this sense, this study presents the latest research trends in the production of lipase biocatalysts and their optimized industrial applications.…”

Section: Introductionmentioning

confidence: 97%

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

Cavalcante

Sousa

et al. 2021

Catalysts

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The market for industrial enzymes has witnessed constant growth, which is currently around 7% a year, projected to reach $10.5 billion in 2024. Lipases are hydrolase enzymes naturally responsible for triglyceride hydrolysis. They are the most expansively used industrial biocatalysts, with wide application in a broad range of industries. However, these biocatalytic processes are usually limited by the low stability of the enzyme, the half-life time, and the processes required to solve these problems are complex and lack application feasibility at the industrial scale. Emerging technologies create new materials for enzyme carriers and sophisticate the well-known immobilization principles to produce more robust, eco-friendlier, and cheaper biocatalysts. Therefore, this review discusses the trending studies and industrial applications of the materials and protocols for lipase immobilization, analyzing their advantages and disadvantages. Finally, it summarizes the current challenges and potential alternatives for lipases at the industrial level.

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Comparison of the immobilization of lipase from Pseudomonas fluorescens on divinylsulfone or p-benzoquinone activated support

Cited by 88 publications

References 56 publications

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

Genipin as An Emergent Tool in the Design of Biocatalysts: Mechanism of Reaction and Applications

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

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