Comparison of the entire nucleotide and deduced amino acid sequences of the attenuated hog cholera vaccine strain GPE? and the wild-type parental strain ALD

Ishikawa, K.; Nagai, Hidetaka; Katayama, Kazuhiko; Tsutsui, Masahiro; Tanabayashi, Kiyoshi; Takeuchi, Kaoru; Hishiyama, Michiko; Saitoh, Akito; Takagi, Masamchi; Gotoh, Koshichi; Muramatsu, Masatake; Yamada, Akio

doi:10.1007/bf01322665

Cited by 48 publications

(27 citation statements)

References 23 publications

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“…No sequence differences were found in the 5Ј and 3Ј NTRs of Brescia C1.1.1 and CoBrB 476 R strains, suggesting that these regions of the genome do not determine the virulence of the two viruses. The amino acid sequences of C1.1.1 (25) and CoBrB 476 R were compared with those of CSFV strains Alfort (26), ALD (14), GPE Ϫ (14), and C (23). Except for the amino acid at position 2846 in nonstructural protein NS5A, the amino acids of virulent CoBrB 476 R and other virulent and avirulent CSFV strains are conserved compared to those of the C1.1.1 sequence (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…These results suggested that the surface properties of CSFV generated in pigs and carrying 476 Ser in the C terminus of E rns are distinct from those of genetically identical virus produced in cell culture. Field isolates and laboratory strains of CSFV vary considerably in their virulence, but until now, no genetic determinants of virulence could be defined despite extensive efforts in sequencing and detailed phylogenetic studies (14,35). The same holds true for the search for in vitro parameters that would allow grouping of viruses into virulence classes.…”

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confidence: 99%

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Determinants of Virulence of Classical Swine Fever Virus Strain Brescia

Gennip

Vlot

Hulst

et al. 2004

J Virol

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Determinants of Virulence of Classical Swine Fever Virus Strain Brescia

Gennip

Vlot

Hulst

et al. 2004

J Virol

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“…A total of 46 amino acid differences were found between the parent ALD virus and the GPE Ϫ vaccine strain (22). Since the pathogenicity of the triple mutant GPE Ϫ virus is not as high as the pathogenicity of the original ALD virus, additional changes are likely to determine the virulence of the ALD strain.…”

Section: Discussionmentioning

confidence: 99%

“…Pigs inoculated with the GPE Ϫ vaccine were completely protected from challenge virus infection and replication (34,49). Comparison of the genomes of the GPE Ϫ vaccine virus and the parent ALD virus revealed 225 nucleotide substitutions and 46 amino acid differences (22), which did not provide any conclusive information on the molecular basis of the attenuation. Numerous reports suggest that the viral proteins N pro , C, E rns , E1, E2, p7, and NS4B may be involved in determining the virulence of CSFV in pigs (7,8,9,10,11,12,13,32,36,37,38,39,40,43,46,54).…”

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confidence: 91%

Selection of Classical Swine Fever Virus with Enhanced Pathogenicity Reveals Synergistic Virulence Determinants in E2 and NS4B

Tamura

Sakoda

Yoshino

et al. 2012

J Virol

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c Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious disease of pigs. There are numerous CSFV strains that differ in virulence, resulting in clinical disease with different degrees of severity. Low-virulent and moderately virulent isolates cause a mild and often chronic disease, while highly virulent isolates cause an acute and mostly lethal hemorrhagic fever. The live attenuated vaccine strain GPE ؊ was produced by multiple passages of the virulent ALD strain in cells of swine, bovine, and guinea pig origin. With the aim of identifying the determinants responsible for the attenuation, the GPE ؊ vaccine virus was readapted to pigs by serial passages of infected tonsil homogenates until prolonged viremia and typical signs of CSF were observed. The GPE ؊ /P-11 virus isolated from the tonsils after the 11th passage in vivo had acquired 3 amino acid substitutions in E2 (T830A) and NS4B (V2475A and A2563V) compared with the virus before passages. Experimental infection of pigs with the mutants reconstructed by reverse genetics confirmed that these amino acid substitutions were responsible for the acquisition of pathogenicity. Studies in vitro indicated that the substitution in E2 influenced virus spreading and that the changes in NS4B enhanced the viral RNA replication. In conclusion, the present study identified residues in E2 and NS4B of CSFV that can act synergistically to influence virus replication efficiency in vitro and pathogenicity in pigs.

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“…A known example is that GPE − vaccine virus was obtained from an ALD strain after multiple passages in swine testicle, bovine testicle and guinea-pig kidney cells [11]. They were not clustered closely in both the E rns and E2 trees.…”

Section: Phylogenetic Analysis Of E Rns and E2 Genesmentioning

confidence: 99%

Phylogenetic analysis of classical swine fever virus in Taiwan

et al. 2005

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Summary. Two envelope glycoprotein (E rns and E2) regions of the classical swine fever virus (CSFV) were amplified by RT-PCR and sequenced directly from 158 specimens collected between 1989 and 2003 in Taiwan. Phylogenetic analysis of the two regions revealed a similar tree topology and the E rns region provided better discrimination than the E2 region. One hundred and fifteen isolates out of the 158 isolates were clustered within subgroup 2.1 (further classified as 2.1a and 2.1b) and 2.2, which were considered to be likely of the introduced strains, whereas the remaining 43 isolates were clustered within subgroup 3.4 and were considered to be of the endemic strains. The subgroup 2.1a viruses were first detected in 1994 and predominated from 1995 onwards. However, subgroup 3.4 viruses were prevalent in the early years, not being isolated after 1996. We have observed a dramatic switch in genotype from subgroup 3.4 to 2.1a. The subgroup 2.1a isolates are closely related to the Paderborn and Lao isolates, whereas 2.1b isolates have a close relationship to the Chinese Guangxi isolates. The phylogenetic tree of 27 CSFV sequences based on the complete envelope glycoprotein gene (E rns -E2) displayed better resolution than that based on the complete open reading frame.

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Comparison of the entire nucleotide and deduced amino acid sequences of the attenuated hog cholera vaccine strain GPE? and the wild-type parental strain ALD

Cited by 48 publications

References 23 publications

Determinants of Virulence of Classical Swine Fever Virus Strain Brescia

Determinants of Virulence of Classical Swine Fever Virus Strain Brescia

Selection of Classical Swine Fever Virus with Enhanced Pathogenicity Reveals Synergistic Virulence Determinants in E2 and NS4B

Phylogenetic analysis of classical swine fever virus in Taiwan

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