Comparison of the efficiency of different NF membranes for the enrichment of milk oligosaccharides from bovine milk

Role of pH in the recovery of bovine milk oligosaccharides from colostrum whey permeate by nanofiltration

“…A previous attempt to isolate BMO from whey permeate using NF by another group reported a final concentration of 0.83 g L −1 (Altmann et al, 2015). A higher final concentration of BMO will facilitate further processing to concentrate the retentate for integration into food products, which may include drying, evaporation, or reverse osmosis.…”

“…Enzymatic hydrolysis of lactose into glucose and galactose prior to NF can improve efficiency and selectivity of membrane-based separations (Goulas, Kapasakalidis, Sinclair, Rastall, & Grandison, 2002). Recent investigations focused on improving the separation of oligosaccharides from monosaccharides at a variety of scales reported low selectivity using membrane filtration (Altmann et al, 2015; Martinez-Ferez et al, 2006; Nordvang et al, 2014). Generally, it has been found that critical operational parameters affecting membrane selectivity and performance include membrane chemistry, molecular-weight cutoff, transmembrane pressure (TMP), pH and solute concentration of the feed, and the use of diafiltration.…”

Bovine milk oligosaccharides as anti-adhesives against the respiratory tract pathogen Streptococcus pneumoniae

“…Compared with concentrations found in whey (0.07%, w/w; Marotta et al unpublished data), this represents an approximate 329 fold SL enrichment in the OSF. Recently, nanofiltration was investigated to enrich BMO from lactose-hydrolysed bovine milk (Altmann et al, 2015) and lactose-hydrolysed colostrum whey permeate (Cohen, Barile, Liu, & de Moura Bell, 2017). Altmann et al (2015) produced a NF retentate containing 873.23 BMO mg L -1 .…”

“…Although the process employed in the present study did not hydrolyse lactose and did not employ NF, as in Altmann et al (2015) and Cohen et al (2017), the OSF was characterised by a much higher content of SL (23%, w/w). The oligosaccharide profile of the OSF was analysed by NIBRT.…”

Discovery of Novel High-Molecular Weight Oligosaccharides Containing N-Acetylhexosamine in Bovine Colostrum Whey Permeate Hydrolyzed with Aspergillus oryzae β-Galactosidase

“…Altmann et al were the first to employ ultrafiltration and nanofiltration along with a β -galactosidase treatment of bovine milk to enrich oligosaccharides. 18 In that work, the membrane process was scaled up from laboratory (0.2 L) to pilot (6 L) and industrial scale (1000 L). The oligosaccharides were enriched by a factor of 100, and the amounts of carbohydrates in nanofiltration retentate were 2.6% oligosaccharides and 28.3% mono- and disaccharides.…”