Comparison of the Efficacy of Three Different Mouthrinse Solutions in Decreasing the Level of Streptococcus Mutans in Saliva

Abstract

ObjectivesThe aim of this study was to evaluate the effectiveness of three different antiseptic mouthrinse solutions on the saliva samples obtained from the individuals, who had high caries activity rate.MethodsThe efficacy of three antiseptic mouthrinses were evaluated in a study with healthy volunteers. The three antiseptic solutions used in this study were 0.1% octenidine dihydrochloride (Octenisept, Schülke&Mayr, UK), 0.12% chlorhexidine digluconate (Kloroben, Drogsan, Turkey) and an antimicrobial enzymatic rinse (Biotene, Laclede, Inc, USA). A total of 27 adult volunteer subjects were participated in the study. The subjects were stratified into three balanced group. Then the mouth rinses were used by each group according to the manufacturer’s directions. The subjects were restricted for 60 minutes for food intake after using the prescribed mouthrinse. The saliva samples were collected from the volunteers at 1, 10 and 60 minutes after their usage in tubes. The tubes were kept in +4°C in a fridge till the evaluation. 10−3 and 10−5 dilutions were prepared for each solution and S. mutans were evaluated according to total number of colony forming unit (CFU) per ml. The dilutions were spreaded on the surface of Brucella agar plates for anaerobic incubation for 48 hours. The dilutions were 100, 10−3 and 10−5 of the solutions Kloroben, Biotene, Octenisept, and the time factor were 0, 1, 10 and 60 minutes. The statistical analyses were performed by Duncan and Bonferroni tests.ResultsOctenisept was found to be more effective over S. mutans than the other mouthrinse solutions (P<.05).ConclusionsAll mouthrinse solutions except Biotene were effective on oral microorganisms.

Antimicrobial efficiency of mouthrinses versus and in combination with different photodynamic therapies on periodontal pathogens in an experimental study

“…This may be due to the CHX concentration of 0.12% used by Kocak et al . , which was lower in comparison to the present study. Comparable minimal bactericidal concentrations for killing oral plaque‐forming microorganisms were found for OCT and CHX in an in vitro study of Slee and O'Connor .…”

The effect of chlorhexidine on plaque index and mutans streptococci in orthodontic patients: A pilot study

“…The Group III baseplates were made with 14.4 g powder and 5.8 g liquid, including addition of 0.12 ml of chlorhexidine digluconate solution at concentration of 20% (Natural Pharma, São João Clímaco, São Paulo, Brazil) to monomer, thus yielding acrylic resin with final concentration of 0.12% chlorhexidine. The amount of antimicrobial agent added to the acrylic resin was determined on the basis of their concentrations in mouth rinses largely used in dentistry clinic, that is, 0.12% chlorhexidine digluconate [12,24]. The patients were requested to wear the orthodontic appliances full time, except during sleep and meals.…”

“…These microorganisms can promote biofilm formation on brackets [4,5], orthodontic wires [6], elastics [7], and acrylic resin [8][9][10]. Results of studies have shown that placement of fixed orthodontic appliance can alter the oral microbiota, increasing plaque volume and levels of MS in saliva [2,5,[11][12][13]. The increased levels of MS may subsequently become a factor favouring the higher incidence of enamel demineralization seen in some patients with fixed orthodontic appliances [14].…”

Clinical Trials with Herbal Products for the Prevention of Dental Caries and Their Quality: A Scoping Study

“…"Antiinfectives and antiseptics for local oral treatment" are grouped under a different subheading of stomatological preparations, because they have a broader range of indications, such as gingivitis, stomatitis, etc. [25], but such products are claimed to be useful inter alia to prevent dental caries [26].…”