Comparison of the Effectiveness of Ovulation Synchronization Protocol in Anestrous and Cycling Beef Cows

Osawa, Takeshi; Morishige, Daisaku; Ohta, Daisaku; Kimura, Yusuke; Hirata, Toh-Ichi; Miyake, Yoh‐Ichi

doi:10.1262/jrd.49.513

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…This protocol is designed such that the plasma estradiol-17β concentration at the time of GnRH injection in the cow is as high as that in the cow at estrous. However, the average value in this study was lower than that reported previously [18]. This result suggests that the 36-h interval between PRID removal and GnRH applied in this study was rather short and so the ovulatory follicle had not matured sufficiently by the time of GnRH injection.…”

Section: Discussioncontrasting

confidence: 83%

Effect of Suckling on Embryo Production by Repeated Ovum Pick-Up Before and After Timed Artificial Insemination in Early Postpartum Japanese Black Cows

Hirata

Sato

Sasaki

et al. 2008

Journal of Reproduction and Development

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Abstract. We investigated whether suckling would affect embryo production of cows bred by timed artificial insemination (TAI) following an ovulation synchronization protocol combined with ovum pick-up and progesterone releasing intravaginal device (OPU-PRID-TAI protocol). The number of oocytes and transferable embryos collected by repeated OPU, performed before and after TAI, were recorded. A total of 14 Japanese Black cows were divided into weaned (n=7) and suckled groups (n=7). All 14 cows were treated with OPU on day 0 (the first day of treatment) and then with a PRID for 9 days. Prostaglandin F2α analog was administered on day 7, GnRH analog was administered on day 10 (36 h after removal of the PRID) and TAI was performed 12 h later. Ovulation was confirmed by palpation per rectum the following day. After TAI, additional OPU sessions were performed on days 18, 25 and 32. The synchronized ovulation rates of the weaned and suckled groups were 100 and 85.7%, and the conception rates were 71.4 and 42.9%, respectively. Immature oocytes were fertilized and cultured in vitro. The numbers of oocytes collected and blastocysts generated were similar between the individual OPU sessions in both groups. However, the total numbers of oocytes collected, cultured oocytes, cleavage embryos and blastocysts as well as the proportions of cleavage embryos and blastocysts to cultured oocytes were all significantly (P<0.05) greater in the weaned group compared with the suckled group. These results suggest that the OPU-PRID-TAI protocol has the potential to produce a significant number of good-quality embryos in vitro after repeated OPU in early postpartum weaned Japanese Black cows. To collect more oocytes and produce more embryos, we suggest that calves be removed from cows scheduled for treatment using this protocol. Key words: Cow, Early postpartum, Japanese black, Ovum pick-up (OPU), Suckling, Timed artificial insemination (TAI) (J. Reprod. Dev. 54: [346][347][348][349][350][351] 2008) t is economically advantageous for beef cattle producers to have cows in estrus within 60 days postpartum and pregnant within 80 days postpartum and to produce a calf each year. An increase in days open results in a significant economic loss, particularly in the case of Japanese Black cows whose calves have a high market value because of the marbled meat they produce. Various estrus and ovulation synchronization programs have been applied to early postpartum beef cows to achieve maximum reproductive ability [1][2][3]. Use of intravaginal progesterone devices in these programs has been effective in animals with silent heat or anestrus [4].Transvaginal ovum pick-up (OPU), a technique for collecting oocytes from live donor cows using ultrasound-guided transvaginal oocyte aspiration, and in vitro embryo production (IVP) have become important methods for making the most of the genetic traits of superior donors in the beef industry [5][6][7][8]. In addition, OPU is applicable to animals in various reproductive states, including the cyclic, non-cycl...

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Section: Discussioncontrasting

confidence: 83%

Effect of Suckling on Embryo Production by Repeated Ovum Pick-Up Before and After Timed Artificial Insemination in Early Postpartum Japanese Black Cows

Hirata

Sato

Sasaki

et al. 2008

Journal of Reproduction and Development

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“…Moreover, the addition of a progesterone-releasing device (a controlled internal device release, CIDR) between days 0 and 7 of the CO-Synch protocol was associated with a higher pregnancy rate (58%) than in cows that lacked a CIDR (48%) [9]. However, another study showed that the prolonged postpartum anestrus limits the efficacy of such synchronization ovulation protocols in Japanese beef cows [14].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of a modified Double-Ovsynch protocol for the enhancement of reproductive performance in Hanwoo cattle

Jeong

Kim

2023

Anim Biosci

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Objective:We aimed to evaluate the efficacy of a modified Double-Ovsynch protocol vs. artificial insemination following estrus detection (AIED) for the enhancement of reproductive performance in Hanwoo cattle. Methods: Four hundred twelve Hanwoo cows were allocated to two treatment groups. The first group of cows were administered gonadotropin releasing hormone (GnRH) on Day 36 (± 0.6), prostaglandin F2α (PGF2α) on Day 46 (8-12 days later), and GnRH on Day 49, which was followed by Ovsynch, consisting an injection of GnRH on Day 56, PGF2α on Day 63, and GnRH 56 h and timed AI (TAI) 16 h later (modified Double-Ovsynch group, n=203). The second group of cows underwent AIED (AIED group, n=209) and were designated as controls. Results:The pregnancy per AI 60 days after the first AI was higher in the modified Double-Ovsynch (68.5%) than in the AIED (56.5%) group, resulting in a higher probability of pregnancy per AI (odds ratio: 1.68, p<0.05). Moreover, cows in the modified Double-Ovsynch group were more likely (hazard ratio: 1.28, p<0.05) to be pregnant by 150 days after calving than cows in the AIED group, and this difference was associated with a lower mean number of AIs per conception (1.27 vs. 1.39, p<0.05) and a shorter median interval between calving and pregnancy (72 vs. 78 days, p<0.1). Conclusion:The modified Double-Ovsynch protocol, adjusted according to the herd visit schedule, can be readily used to increase the pregnancy per AI following the first AI and to shorten the interval between calving and pregnancy in beef herds.

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“…Complete uterine involution takes more time than ovarian function, and the uterus is usually not ready for the embryo to implant until approximately 5 weeks postpartum in cows. When the ovaries start functioning normally, and uterine involution becomes complete, some cows can conceive by artificial insemination (AI) after estrous detection or a timed AI protocol without estrous detection [ 3 , 4 , 5 , 6 , 7 , 8 ]. However, some other cows cannot get pregnant because the uterine environment is not yet ready to accept the embryo for implantation because of persistent intrauterine inflammation beyond the puerperium.…”

Section: Introductionmentioning

confidence: 99%

Predisposing factors, diagnostic and therapeutic aspects of persistent endometritis in postpartum cows

Osawa

2021

Journal of Reproduction and Development

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A certain level of endometrial bacterial infection and inflammation is involved in bovine uterine involution during the puerperal period. Factors that hamper normal uterine involution expose the uterine environment to pathological conditions, causing different endometritis levels. The lack of proper diagnostic tools extends the time to conception. Efforts have been made to elucidate the postpartum uterine environment, including bacterial flora, changes in transient endometrial inflammation, and the pathophysiology of endometritis, to improve bovine reproductive performance. E. coli and Trueperella pyogenes in the uterus are likely to cause persistent infection, and Mycoplasma bovigenitalium infection is associated with dystocia and cytological endometritis in postpartum dairy cows. Due to the widespread use of cytobrush as a diagnostic tool for bovine subclinical endometritis (SE) that enables quantification of the degree of inflammation, we found that endometritis at week 5 postpartum was associated with delayed first ovulation. Approximately 30% of open cows have SE during the postpartum period, and cows with low blood glucose during prepartum have a high risk of developing SE. Additionally, cows with purulent vaginal discharge do not always have endometritis but only vaginitis and/or cervicitis. Intrauterine infusion of polyvinylpyrrolidone-iodine (PVP-I)improves fertility and promotes endometrial epithelial cell regeneration after inducing transient uterine inflammation, suggesting that PVP-I could be a good alternative to antibiotics. In conclusion, prepartum management to prevent glucose deficiency, prompt diagnosis to identify causative agents and intrauterine inflammation levels, and appropriate treatment to minimize antimicrobial resistance is beneficial for tackling endometritis and improving reproductive performance in bovine herds.

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Comparison of the Effectiveness of Ovulation Synchronization Protocol in Anestrous and Cycling Beef Cows

Cited by 6 publications

References 22 publications

Effect of Suckling on Embryo Production by Repeated Ovum Pick-Up Before and After Timed Artificial Insemination in Early Postpartum Japanese Black Cows

Effect of Suckling on Embryo Production by Repeated Ovum Pick-Up Before and After Timed Artificial Insemination in Early Postpartum Japanese Black Cows

Efficacy of a modified Double-Ovsynch protocol for the enhancement of reproductive performance in Hanwoo cattle

Predisposing factors, diagnostic and therapeutic aspects of persistent endometritis in postpartum cows

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