Comparison of the direct burst pressure and the ring tensile test methods for mechanical characterization of tissue-engineered vascular substitutes

Laterreur, Véronique; Ruel, Jean; Auger, François A.; Vallières, Karine; Tremblay, Caroline; Lacroix, Dan; Tondreau, Maxime Y.; Bourget, Jean-Michel; Germain, Lucie

doi:10.1016/j.jmbbm.2014.02.017

Cited by 58 publications

(36 citation statements)

References 32 publications

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“…UTS and ultimate strain were defined by the peak stress and maximum deformation withstood by the samples prior to failure. The estimated burst pressure (BP estimated , MPa) was calculated from the tensile tests results by rearranging Laplace's law for a pressurized thin-walled hollow cylinder as described previously [24]:

\begin{matrix} T1 \\ {B P}_{e s t i m a t e d} = \frac{F_{m a x}}{w \times I D}, \end{matrix}

where F max is the maximal load at failure (N), ID is the corresponding inner diameter at failure (mm), and w is the length of the ring (mm). The resulting burst pressure is converted to mmHg by multiplying by 7500.62 mmHg/MPa.…”

Section: Methodsmentioning

confidence: 99%

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In VivoRemodeling of Fibroblast-Derived Vascular Scaffolds Implanted for 6 Months in Rats

Tondreau

Laterreur

Vallières

et al. 2016

BioMed Research International

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There is a clinical need for tissue-engineered small-diameter (<6 mm) vascular grafts since clinical applications are halted by the limited suitability of autologous or synthetic grafts. This study uses the self-assembly approach to produce a fibroblast-derived decellularized vascular scaffold (FDVS) that can be available off-the-shelf. Briefly, extracellular matrix scaffolds were produced using human dermal fibroblasts sheets rolled around a mandrel, maintained in culture to allow for the formation of cohesive and three-dimensional tubular constructs, and decellularized by immersion in deionized water. The FDVSs were implanted as an aortic interpositional graft in six Sprague-Dawley rats for 6 months. Five out of the six implants were still patent 6 months after the surgery. Histological analysis showed the infiltration of cells on both abluminal and luminal sides, and immunofluorescence analysis suggested the formation of neomedia comprised of smooth muscle cells and lined underneath with an endothelium. Furthermore, to verify the feasibility of producing tissue-engineered blood vessels of clinically relevant length and diameter, scaffolds with a 4.6 mm inner diameter and 17 cm in length were fabricated with success and stored for an extended period of time, while maintaining suitable properties following the storage period. This novel demonstration of the potential of the FDVS could accelerate the clinical availability of tissue-engineered blood vessels and warrants further preclinical studies.

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\begin{matrix} T1 \\ {B P}_{e s t i m a t e d} = \frac{F_{m a x}}{w \times I D}, \end{matrix}

Section: Methodsmentioning

confidence: 99%

“…The unloaded ID was determined as the diameter when the recorded load significantly exceeds the zero load state. The elastic modulus was defined as the slope of the linear portion of the stress-strain curve in the 30–80% range of the UTS of the sample [24]. …”

Section: Methodsmentioning

confidence: 99%

In VivoRemodeling of Fibroblast-Derived Vascular Scaffolds Implanted for 6 Months in Rats

Tondreau

Laterreur

Vallières

et al. 2016

BioMed Research International

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“…The wall-thickness of the TEVS was measured as described previously [29] using a high accuracy CCD digital micrometer (Keyence, Pointe-Claire, Quebec, Canada). Thickness measurements were performed on seven substitutes for each cell population used (n = 7 per population; n = 21 per cell type and N = 3 populations per cell type).…”

Section: Thickness Measurementsmentioning

confidence: 99%

“…The TEVS were subjected to ring tensile testing on an Instron ElectroPuls E1000 mechanical tester (Instron Corporation, Norwood, MA) along with a ±50 N load cell (Instron Corporation) as described previously [29]. Briefly, 5-mm-long ring samples were cut from the substitutes and mounted between two hooks adapted to the mechanical tester.…”

Section: Uniaxial Tensile Testingmentioning

confidence: 99%

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Human adipose-derived stromal cells for the production of completely autologous self-assembled tissue-engineered vascular substitutes

et al. 2015

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Development of a Novel Hierarchically Biofabricated Blood Vessel Mimic Decorated with Three Vascular Cell Populations for the Reconstruction of Small‐Diameter Arteries

Carrabba,

Fagnano,

Ghorbel

et al. 2023

Adv Funct Materials

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The availability of grafts to replace small‐diameter arteries remains an unmet clinical need. Here, the validated methodology is reported for a novel hybrid tissue‐engineered vascular graft that aims to match the natural structure of small‐size arteries. The blood vessel mimic (BVM) comprises an internal conduit of co‐electrospun gelatin and polycaprolactone (PCL) nanofibers (corresponding to the tunica intima of an artery), reinforced by an additional layer of PCL aligned fibers (the internal elastic membrane). Endothelial cells are deposited onto the luminal surface using a rotative bioreactor. A bioprinting system extrudes two concentric cell‐laden hydrogel layers containing respectively vascular smooth muscle cells and pericytes to create the tunica media and adventitia. The semi‐automated cellularization process reduces the production and maturation time to 6 days. After the evaluation of mechanical properties, cellular viability, hemocompatibility, and suturability, the BVM is successfully implanted in the left pulmonary artery of swine. Here, the BVM showed good hemostatic properties, capability to withstand blood pressure, and patency at 5 weeks post‐implantation. These promising data open a new avenue to developing an artery‐like product for reconstructing small‐diameter blood vessels.

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Comparison of the direct burst pressure and the ring tensile test methods for mechanical characterization of tissue-engineered vascular substitutes

Cited by 58 publications

References 32 publications

In VivoRemodeling of Fibroblast-Derived Vascular Scaffolds Implanted for 6 Months in Rats

In VivoRemodeling of Fibroblast-Derived Vascular Scaffolds Implanted for 6 Months in Rats

Human adipose-derived stromal cells for the production of completely autologous self-assembled tissue-engineered vascular substitutes

Development of a Novel Hierarchically Biofabricated Blood Vessel Mimic Decorated with Three Vascular Cell Populations for the Reconstruction of Small‐Diameter Arteries

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