Comparison of the content and subpopulations of CD3 and CD34 positive cells in bone marrow harvests and G-CSF-mobilized peripheral blood leukapheresis products from healthy adult donors

Hassan, H. T.; Stockschläder, M; Schleimer, B.; Krüger, William; Zander, A.R.

doi:10.1016/s0966-3274(96)80054-2

Cited by 26 publications

(17 citation statements)

References 14 publications

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“…12,22,40 In contrast, in myeloablative PBCS transplantation this inversion does not occur 12,41,42 but values as high as we have seen have not been reported. As the CD4 : CD8 ratio in the patients before transplants was low and the CD4 : CD8 ratio in PBSC leukapheresis products is reported to be around 1.2, 43,44 there must be a preferential proliferation of CD4+ T cells in the first week posttransplant, or a preferential displacement of CD4+ T cells from the lymphoid organs to the intravascular compartment.…”

Section: Discussionmentioning

confidence: 99%

A comparison of T-, B- and NK-cell reconstitution following conventional or nonmyeloablative conditioning and transplantation with bone marrow or peripheral blood stem cells from human leucocyte antigen identical sibling donors

Petersen

Ryder

Björk

et al. 2003

Bone Marrow Transplant

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Summary:This retrospective study compares the reconstitution of T, B and NK cells in three groups of patients transplanted for haematological malignancies with grafts from their HLAidentical sibling donors. In all, 15 patients received PBSC after a nonmyeloablative conditioning regimen consisting of fludarabine and 200 cGy TBI, 13 patients received PBSC after myeloablative conditioning and 37 patients received BM after myeloablative conditioning. In the nonmyeloablative group, the NK cells normalised after 1 month, the CD8+ T cells normalised after 3 months, the CD4+ T cells reached near normal values after 9 months and the B cell values were reduced until 12 months after transplant. In the two myeloablative groups, recipients of PBSC had a significantly higher number of CD4+ T cells after 4 months (P ¼ 0.004) and after 12 months (P ¼ 0.001), than recipients of BM. We found no differences in the T cell reconstitution between the two PBSC groups. This was of interest as the recipients of nonmyeloablative conditioning were older (Po0.001) and had a higher occurrence of chronic GVHD (Po0.05) than the recipients of myeloablative conditioning. In contrast, the recipients of nonmyeloablative conditioning had a delayed B cell recovery when compared to the patients who received myeloablative conditioning (P ¼ 0.04). Bone Marrow Transplantation (2003) 32, 65-72. doi:10.1038/sj.bmt.1704084 Keywords: immune reconstitution; nonmyeloablative conditioning regimen; peripheral blood stem cell transplantation Allogeneic haematopoietic stem cell transplantation after nonmyeloablative conditioning is a treatment modality increasingly used for patients with haematological malignancies ineligible for conventional myeloablative BMT because of older age, comorbidity or the high treatmentrelated mortality after conventional BMT in particular diseases. The results of nonmyeloablative stem cell transplantation have so far been promising. 1,2 One advantage of the use of a nonmyeloablative conditioning regimen is the brief granulo-and thrombocytopenia, which allows the procedure to be performed in an outpatient setting. The reconstitution of the T, B and NK cells after stem cell transplantation is however also important for the defence against pathogens, 3 and low counts of CD4+ T cells and of B cells have been associated with an increased risk of infection. 4,5 Thymic function is impaired after conventional haematopoietic stem cell transplantation, and this is partially because of the high-dose radiochemotherapy included in the conditioning regimen. 6 As the thymic activity post-transplant has been shown to be important for the generation of a diverse and efficient T cell response, 7,8 the lower toxicity of a nonmyeloablative conditioning regimen could in theory enhance the T cell reconstitution. In this study, we retrospectively compared the reconstitution of absolute numbers of T, B and NK cells in patients transplanted with PBSC and conditioned with a nonmyeloablative regimen to patients who received conventional myeloablative conditio...

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Section: Discussionmentioning

confidence: 99%

A comparison of T-, B- and NK-cell reconstitution following conventional or nonmyeloablative conditioning and transplantation with bone marrow or peripheral blood stem cells from human leucocyte antigen identical sibling donors

Petersen

Ryder

Björk

et al. 2003

Bone Marrow Transplant

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“…The differences in neutrophil and platelet reconstitution kinetics are statistically significant in all studies reported. 14 (n ϭ 111) 24.1 (10-110) 34 (n ϭ 26)…”

Section: Neutrophil and Platelet Reconstitutionmentioning

confidence: 99%

Peripheral blood stem cell versus bone marrow allotransplantation: does the source of hematopoietic stem cells matter?

Körbling

Anderlini

2001

Blood

317

205

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“…The CD34 + , CD34 + CD41 + and CD41 + cells were determined as previously described [34,35]. Briefly, 1 × 10 6 cells from each sample on days 0, 7, and 14 were incubated for 30 min at 4°C in darkness with the phycoerythrin (PE)-conjugated mAb anti-CD34 or anti-CD41 and fluorescein isothiocyanate (FITC)-conjugated anti-CD45 obtained from Coulter (Bedford, UK) then washed twice with PBS/1% bovine serum albumin.…”

Section: Flow Cytometry Measurement Of Cd34 + Cd34 + Cd41 + and Cmentioning

confidence: 99%

Cytokine‐Induced Expansion of Human CD34⁺Stem/Progenitor and CD34⁺CD41⁺Early Megakaryocytic Marrow Cells Cultured on Normal Osteoblasts

1999

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Comparison of the content and subpopulations of CD3 and CD34 positive cells in bone marrow harvests and G-CSF-mobilized peripheral blood leukapheresis products from healthy adult donors

Cited by 26 publications

References 14 publications

A comparison of T-, B- and NK-cell reconstitution following conventional or nonmyeloablative conditioning and transplantation with bone marrow or peripheral blood stem cells from human leucocyte antigen identical sibling donors

A comparison of T-, B- and NK-cell reconstitution following conventional or nonmyeloablative conditioning and transplantation with bone marrow or peripheral blood stem cells from human leucocyte antigen identical sibling donors

Peripheral blood stem cell versus bone marrow allotransplantation: does the source of hematopoietic stem cells matter?

Cytokine‐Induced Expansion of Human CD34⁺Stem/Progenitor and CD34⁺CD41⁺Early Megakaryocytic Marrow Cells Cultured on Normal Osteoblasts

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Comparison of the content and subpopulations of CD3 and CD34 positive cells in bone marrow harvests and G-CSF-mobilized peripheral blood leukapheresis products from healthy adult donors

Cited by 26 publications

References 14 publications

A comparison of T-, B- and NK-cell reconstitution following conventional or nonmyeloablative conditioning and transplantation with bone marrow or peripheral blood stem cells from human leucocyte antigen identical sibling donors

A comparison of T-, B- and NK-cell reconstitution following conventional or nonmyeloablative conditioning and transplantation with bone marrow or peripheral blood stem cells from human leucocyte antigen identical sibling donors

Peripheral blood stem cell versus bone marrow allotransplantation: does the source of hematopoietic stem cells matter?

Cytokine‐Induced Expansion of Human CD34+Stem/Progenitor and CD34+CD41+Early Megakaryocytic Marrow Cells Cultured on Normal Osteoblasts

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Cytokine‐Induced Expansion of Human CD34⁺Stem/Progenitor and CD34⁺CD41⁺Early Megakaryocytic Marrow Cells Cultured on Normal Osteoblasts