Peripheral blood stem cell versus bone marrow allotransplantation: does the source of hematopoietic stem cells matter?

Regeneration, health status and quality of life after rhG-CSF-stimulated stem cell collection in healthy donors: a cross-sectional study

“…[20][21][22] However, administration of rhG-CSF leads to high serum levels in individuals, exceeding endogenous G-CSF levels measured under physiological and pathophysiological conditions, leading to adverse events in a number of them. 7 In contrast to BM, which is collected under general anesthesia by multiple aspirates from the iliac crest, a good peripheral venous access is a requirement for successful PBPC collection.…”

Primitive human hematopoietic cells give rise to differentially specified daughter cells upon their initial cell division

“…Here, we analyzed primitive human hematopoietic cells in bulk cultures and at a single-cell level, and report 3 major findings: (1) we realized that upon cultivation CD34 ϩ cells split up into a CD34 ϩ CD133 ϩ and a CD34 ϩ CD133 Ϫ fraction; (2) using our newly established single-cell separation approach we determined the cell fate of primitive human hematopoietic cells and their first-generation daughter cells individually and present evidence for the existence of progenitor cells containing the capacity to form NK cells and macrophages, but which lack more primitive myeloid capacities (LTC-ICs); and (3) we demonstrate that the most primitive ML-ICs have a high tendency (87%) to transmit their cell fate to only 1 of the arising daughter cells. In contrast, the ratio of more-committed progenitor cells giving rise to progeny adopting identical cell fates to those adopting different cell fates is more balanced.…”

“…More than 30 years of clinical experience as well as experiments with animals demonstrated that neonatal and adult HSCs retain the ability to reconstitute the hematopoietic systems of patients after myeloablative treatment. 1 Therefore, an important feature of HSCs is the capacity to replenish all lineages of mature blood cells. Beside this, they also have the potential to expand in vivo as revealed by sequential transplantation experiments using limiting numbers of mouse HSCs to reconstitute the hematopoietic systems of primary and secondary lethally irradiated hosts.…”

Reduced risk of acute GVHD following mobilization of HLA-identical sibling donors with GM-CSF alone

“…[1][2][3][4][5][6][7][8] Nevertheless, in only a minority of the randomized comparative trials did this translate into an overall survival advantage and some studies suggested the use of G-CSF mobilized PBPC was associated with a greater risk of either acute or chronic graft-versus-host disease (GVHD), or both. 6,[8][9][10] In two recent retrospective studies in patients with aplastic anemia or children with leukemia performed by the International Bone Marrow Transplant Registry, transplantation of G-CSF mobilized PBPC was associated with higher rates of GVHD. 11,12 Therefore, strategies to limit GVHD following PBPC transplantation are necessary.…”