Comparison of the cobas Human Immunodeficiency Virus 1 (HIV-1) Test Using the cobas 4800 System With COBAS AmpliPrep/COBAS TaqMan HIV-1 Test and Abbott RealTime HIV-1 Assay and Performance Evaluation of cobas HIV-1

Shin, Kyung‐Hwa; Lee, Hyunji; Lee, Jin Hyeon; Chang, Chulhun L.; Kim, Hyung Hoi

doi:10.1093/ajcp/aqz075

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…The HIV-1 viral loads in these patients’ plasma were re-tested with m2000sp Liquid Handler & m 2000 rt Real Time PCR System (Abbott) following the manufacturer’s protocol, which could be used to evaluate amplification sensitivity for AP in-house method [ 46 , 47 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a Novel In-house HIV-1 Genotype Drug Resistance Assay using Clinical Samples in China

Gao

Yang

et al. 2022

CHR

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Background: HIV drug resistance poses a major challenge for anti-retroviral treatment (ART) and the prevention and control of HIV epidemic. Objective: The study aims to establish a novel in-house assay with high efficiency, named AP in-house method, that would be suitable for HIV-1 drug resistance detection in China. Methods: An in-house HIV-1 genotyping method was used to sequence the partial pol gene from 60 clinical plasma samples; the results of our test were compared with a commercial ViroSeq HIV-1 genotyping system. Results : Among sixty samples, 58(96.7%) were successfully amplified by AP in-house method, five of them harbored viral load below 1,000 copies/ml. The genotype distribution was 43.1% CRF07_BC (25/58), 39.7% CRF01_AE (23/58), 6.9% CRF55_01B (4/58), 5.2% subtype B (3/58) and 5.2% CRF08_BC (3/58). Compared with that of the ViroSeq system, the consistent rate of these nucleotides and amino acids obtained by AP in-house method was up to 99.5 ± 0.4% and 99.5 ± 0.4%, respectively. A total of 290 HIV-1 drug resistance mutations were identified by two methods, including 126 nucleoside reverse transcriptase inhibitors (NRTIs), 145 non-nucleoside reverse transcriptase inhibitors (NNRTIs) and 19 protease inhibitors (PIs) resistance mutations. Out of them, 94.1% (273/290) were completely concordant between the AP in-house method and the ViroSeq system. Conclusion: Overall, the evaluation of AP in-house method provided comparable results to those of the ViroSeq system on diversiﬁed HIV-1 subtypes in China.

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Section: Methodsmentioning

confidence: 99%

Evaluation of a Novel In-house HIV-1 Genotype Drug Resistance Assay using Clinical Samples in China

Gao

Yang

et al. 2022

CHR

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“…This target system targeting both the HIV-1 gag p24 gene and the LTR region. 1000 µl of specimens and controls were used using the HI2QLD48 program [6,7].…”

Section: Early Hiv-1 Diagnosis Among Infantsmentioning

confidence: 99%

Mother-child HIV-1 transmission in the departments of Mono and Couffo in Benin: incidence and associated factors in 2019

Koudokpon

Dougnon

Kissira

et al. 2020

Preprint

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Background: Despite worldwide success of introducing HIV testing and treatment with antiretroviral drugs, HIV remains a real public health problem. Vertical mother-child transmission is a form responsible for many cases of new infections. This study was therefore carried out in order to reduce the prevalence of mother-to-child transmission of HIV-1 in the departments of Mono and Couffo. Method: For this purpose, a total of 374 dry blood spots (DBS) were collected from infants born to HIV-positive mothers during the year 2019, and these DBS were used for the molecular diagnosis of HIV1 in these infants. Information on the type of treatment, the type of diet of infants was also collected. The viral loads of the mothers before delivery were determined after the last antenatal visits. To do so, a venous blood sample was taken from an EDTA tube. These different samples were used for DNA extraction. The early diagnosis of these infants as well as the determination of the viral load were carried out by RT-PCR using the Roche automated system. The results were analyzed with the chi-square test on the SPSS software. Result: The results showed that 51% of the newborns included in the study were male and the vast majority of the newborns were in the 0-6 month age group. 93.41% of the newborns included in this study were tested negative for early detection by RT-PCR. Significant links were observed between the early diagnosis of newborns and the mother's mode of feeding, the PMTCT protocol, and the viral load of the mothers. Monoprophylaxis was predominant in the newborn PMTCT protocol (96.28%). Conclusion: The national program for the prevention of vertical transmission of HIV from mother to child deserves to be continued and strengthened with the objective of zero newborns infected at birth in the departments of Mono and Couffo.

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Comparison of the cobas Human Immunodeficiency Virus 1 (HIV-1) Test Using the cobas 4800 System With COBAS AmpliPrep/COBAS TaqMan HIV-1 Test and Abbott RealTime HIV-1 Assay and Performance Evaluation of cobas HIV-1

Cited by 2 publications

References 10 publications

Evaluation of a Novel In-house HIV-1 Genotype Drug Resistance Assay using Clinical Samples in China

Evaluation of a Novel In-house HIV-1 Genotype Drug Resistance Assay using Clinical Samples in China

Mother-child HIV-1 transmission in the departments of Mono and Couffo in Benin: incidence and associated factors in 2019

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