Comparison of the characteristics of macrophages derived from murine spleen, peritoneal cavity, and bone marrow

Zhao, Yang; Tian, Puxun; Han, Feng; Zheng, Jin; Xia, Xinxin; Xue, Wujun; Ding, Xiaoming; Ding, Chenguang

doi:10.1631/jzus.b1700003

Cited by 64 publications

(43 citation statements)

References 30 publications

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“…In contrast to our findings, previous studies have failed to show increase in glucose uptake in resident peritoneal macrophages activated with IFNγ plus TNF (55). This discrepancy can be explained by functional differences between macrophage sources including the magnitude of their responses to pro-inflammatory and anti-inflammatory stimuli (56,57). Macrophages can also be activated by other stimuli including pathogen-associated molecular patterns (PAMPs) such as LPS.…”

Section: Discussioncontrasting

confidence: 99%

Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis

et al. 2020

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Altered lipid metabolism in macrophages is associated with various important inflammatory conditions. Although lipid metabolism is an important target for therapeutic intervention, the metabolic requirement involved in lipid accumulation during pro-inflammatory activation of macrophages remains incompletely characterized. We show here that macrophage activation with IFNγ results in increased aerobic glycolysis, iNOS-dependent inhibition of respiration, and accumulation of triacylglycerol. Surprisingly, metabolite tracing with 13 C-labeled glucose revealed that the glucose contributed to the glycerol groups in triacylglycerol (TAG), rather than to de novo synthesis of fatty acids. This is in stark contrast to the otherwise similar metabolism of cancer cells, and previous results obtained in activated macrophages and dendritic cells. Our results establish a novel metabolic pathway whereby glucose provides glycerol to the headgroup of TAG during classical macrophage activation.

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Section: Discussioncontrasting

confidence: 99%

Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis

et al. 2020

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“…As shown in Fig. 2j , approximately 10% of splenocytes were macrophages (F4/80), most of which were of the M1 phenotype, in agreement with previous data published elsewhere [ 21 ]. B cells constituted approximately 30% of the splenocytes (data not shown).…”

Section: Resultssupporting

confidence: 92%

Resolution of bleomycin-induced murine pulmonary fibrosis via a splenic lymphocyte subpopulation

et al. 2018

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BackgroundIdiopathic pulmonary fibrosis (IPF) is a progressive disease with high mortality, and the pathogenesis of the disease is still incompletely understood. Although lymphocytes, especially CD4+CD25+FoxP3+ regulatory T cells (Tregs), have been implicated in the development of IPF, contradictory results have been reported regarding the contribution of Tregs to fibrosis both in animals and humans. The aim of this study was to investigate whether a specific T cell subset has therapeutic potential in inhibiting bleomycin (BLM)-induced murine pulmonary fibrosis.MethodsC57BL/6 mice received BLM (100 mg/kg body weight) with osmotic pumps (day 0), and pulmonary fibrosis was induced. Then, splenocytes or Tregs were adoptively transferred via the tail vein. The lungs were removed and subjected to histological and biochemical examinations to study the effects of these cells on pulmonary fibrosis, and blood samples were collected by cardiac punctures to measure relevant cytokines by enzyme-linked immunosorbent assay. Tregs isolated from an interleukin (IL)-10 knock-out mice were used to assess the effect of this mediator. To determine the roles of the spleen in this model, spleen vessels were carefully cauterized and the spleen was removed either on day 0 or 14 after BLM challenge.ResultsSplenocytes significantly ameliorated BLM-induced pulmonary fibrosis when they were administered on day 14. This effect was abrogated by depleting Tregs with an anti-CD25 monoclonal antibody. Adoptive transfer of Tregs on day 14 after a BLM challenge significantly attenuated pulmonary fibrosis, and this was accompanied by decreased production of fibroblast growth factor (FGF) 9-positive cells bearing the morphology of alveolar epithelial cells. In addition, BLM-induced plasma IL-10 expression reverted to basal levels after adoptive transfer of Tregs. Moreover, BLM-induced fibrocyte chemoattractant chemokine (CC motif) ligand-2 production was significantly ameliorated by Treg adoptive transfer in lung homogenates, accompanied by reduced accumulation of bone-marrow derived fibrocytes. Genetic ablation of IL-10 abrogated the ameliorating effect of Tregs on pulmonary fibrosis. Finally, splenectomy on day 0 after a BLM challenge significantly ameliorated lung fibrosis, whereas splenectomy on day 14 had no effect.ConclusionsThese findings warrant further investigations to develop a cell-based therapy using Tregs for treating IPF.

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“…Clinical and experimental evidence indicated that aberrant memory B cells and Tfh cells played an important role in the pathogenesis of human SLE [24][25][26]. Resting M0 macrophages can polarize into M1 and M2 macrophages in the presence of the appropriate cytokines [27]. However, no research has explained the function of increased M0 macrophages in LN.…”

Section: Discussionmentioning

confidence: 99%

Immune cell infiltration characteristics and related core genes in lupus nephritis: results from bioinformatic analysis

Cao

Tang

2019

Preprint

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Background Lupus nephritis (LN) is a common complication of systemic lupus erythematosus that presents a high risk of end-stage renal disease. In the present study, we used CIBERSORT and gene set enrichment analysis (GSEA) of gene expression profiles to identify immune cell infiltration characteristics and related core genes in LN. Results Datasets from the Gene Expression Omnibus, GSE32591 and GSE113342, were downloaded for further analysis. The GSE32591 dataset, which included 32 LN glomerular biopsy tissues and 14 glomerular tissues from living donors, was analyzed by CIBERSORT. Different immune cell types in LN were analyzed by the Limma software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis based on GSEA were performed by clusterProfiler software. Lists of core genes were derived from Spearman correlation between the most significant GO term and differentially expressed immune cell gene from CIBERSORT. GSE113342 was employed to validate the association between selected core genes and clinical manifestation. Five types of immune cells revealed important associations with LN, and monocytes emerged as having the most prominent differences. GO and KEGG analyses indicated that immune response pathways are significantly enriched in LN. The Spearman correlation indicated that 15 genes, including FCER1G, CLEC7A, MARCO, CLEC7A, PSMB9, and PSMB8 , were closely related to clinical features. Conclusions This study is the first to identify immune cell infiltration with microarray data of glomeruli in LN by using CIBERSORT analysis and provides novel evidence and clues for further research of the molecular mechanisms of LN.

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Comparison of the characteristics of macrophages derived from murine spleen, peritoneal cavity, and bone marrow

Cited by 64 publications

References 30 publications

Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis

Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis

Resolution of bleomycin-induced murine pulmonary fibrosis via a splenic lymphocyte subpopulation

Immune cell infiltration characteristics and related core genes in lupus nephritis: results from bioinformatic analysis

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