Comparison of the boronic acid disk potentiation test and cefepime–clavulanic acid method for the detection of ESBL among AmpC-producing Enterobacteriaceae

Shoorashetty, RM; Nagarathnamma, T; J, Prathibha

doi:10.4103/0255-0857.83917

Cited by 16 publications

(7 citation statements)

References 24 publications

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“…Among 259 clinical isolates of Enterobacteriaceae , 68 (26.25%) and 14(5.4%) were pure ESBL and AmpC producers respectively; 53 (20.46%) isolates were combined ESBL and AmpC producers; and 124 (47.87%) of the isolates did not harbor any type of enzyme (Table 1). In our study the prevalence of ESBL and AmpC co- producing isolates was 20.46%, which is relatively low (27.5% and 33.7%) compared to the previous report [25, 26]. This variation may be due to different pattern of antibiotic use and differences in the study group.…”

Section: Resultscontrasting

confidence: 62%

Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method

Bakthavatchalu¹,

Shakthivel²,

Mishra³

2013

Pan Afr Med J

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IntroductionThe occurrence of multiple β-lactamases among bacteria only limits the therapeutic options but also poses a challenge. A study using boronic acid (BA), an AmpC enzyme inhibitor, was designed to detect the combined expression of AmpC β-lactamases and extended-spectrum β-lactamases (ESBLs) in bacterial isolates further different phenotypic methods are compared to detect ESBL and AmpC.MethodsA total of 259 clinical isolates of Enterobacteriaceae were isolated and screened for ESBL production by (i) CLSI double-disk diffusion method (ii) cefepime- clavulanic acid method (iii) boronic disk potentiation method. AmpC production was detected using cefoxitin alone and in combination with boronic acid and confirmation was done by three dimensional disk methods. Isolates were also subjected to detailed antibiotic susceptibility test.ResultsAmong 259 isolates, 20.46% were coproducers of ESBL and AmpC, 26.45% were ESBL and 5.40% were AmpC. All of the 53 AmpC and ESBL coproducers were accurately detected by boronic acid disk potentiation method.ConclusionThe BA disk test using Clinical and Laboratory Standards Institute methodology is simple and very efficient method that accurately detects the isolates that harbor both AmpCs and ESBLs.

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Section: Resultscontrasting

confidence: 62%

Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method

Bakthavatchalu¹,

Shakthivel²,

Mishra³

2013

Pan Afr Med J

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“…In our study, we found 64.50% isolates to be pure ESBL producers and 12.70% isolates to be pure AmpC producers and 8.39% were ESBL + AmpC coproducers. Shoorashetty et al reported 6% and 41% isolates to be pure AmpC and pure ESBL producers, respectively, with 27.5% isolates to be AmpC and ESBL coproducers [18]. Bakthavatchalu et al reported 5.4% and 26.25% pure AmpC and pure ESBL producers, respectively, with 20.46% AmpC and ESBL coproducers [19].…”

Section: Discussionmentioning

confidence: 99%

Coexistence of β-Lactamases in Community-Acquired Infections in a Tertiary Care Hospital in India

Mirza

Jadhav

Misra

et al. 2019

International Journal of Microbiology

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Introduction The trends of β-lactamases producing Enterobacteriaceae is ever increasing, and limited studies have reported investigating coexistence of β lactamases in Enterobacteriaceae. A cross-sectional study after approval from the Institutional Ethical committee was conducted between June 2014 and May 2016 in community-acquired infections due to multidrug-resistant organisms in our tertiary care. Nonrepetitive clinical samples from the out-patient department (OPD) were processed for bacteriological culture and identification of Enterobacteriaceae. An antibiotic susceptibility test, screening, and phenotypic confirmation for ESBLs and carbapenemases and AmpC producers were performed to check for coexistence of these enzymes. Results Nonrepetitive clinical specimens processed for culture and identification in our hospital revealed 417 positive isolates in community acquired infections which were multidrug-resistant organisms, and on screening for β-lactamases, 293 isolates were positive for one of the three beta lactamases, ESBL, AmpC, or carbapnemases. Coproduction of ESBL and MBL was seen in 5 isolates, 35 isolates showed coproduction of ESBL and AmpC enzymes, and AmpC and MBL coproduction was exhibited in only in 5 isolates. Conclusions Coexistence of ESBLs, AmpC producers, and carbapenemases has been described. Continuous monitoring and surveillance and proper infection control and prevention practices will limit the further spread of these superbugs within the hospital and beyond.

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“…However, β-lactamase-producing Enterobacteriaceae are commonly cross-resistant to other classes of antibiotics, such as fluoroquinolones, trimethoprim/sulfamethoxazole, and aminoglycosides, which results in limited therapeutic options to treat infections caused by these pathogens [3]. Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase are important mechanisms of beta-lactam resistance among Enterobacteriaceae [5]. Carbapenemases are β-lactamases and able to hydrolize β-lactam antibiotics, including carbapenems and also become a significant resistance mechanism in Enterobacteriaceae [6].…”

Section: Introductionmentioning

confidence: 99%

Resistance Patterns and Phenotypic Detection of β-lactamase Enzymes among Enterobacteriaceae Isolates from Referral Hospitals in Khartoum State, Sudan

Dirar

Bilal

Ibrahim

et al. 2020

Cureus

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Comparison of the boronic acid disk potentiation test and cefepime–clavulanic acid method for the detection of ESBL among AmpC-producing Enterobacteriaceae

Abstract: The use of boronic acid in disk diffusion testing along with the CLSI described PCT enhances ESBL detection in the presence of AmpC betalactamases.

Cited by 16 publications

References 24 publications

Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method

Detection of ESBL among ampc producing enterobacteriaceae using inhibitor-based method

Coexistence of β-Lactamases in Community-Acquired Infections in a Tertiary Care Hospital in India

Resistance Patterns and Phenotypic Detection of β-lactamase Enzymes among Enterobacteriaceae Isolates from Referral Hospitals in Khartoum State, Sudan

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