Comparison of the bone protective effects of an isoflavone-rich diet with dietary and subcutaneous administrations of genistein in ovariectomized rats

Hertrampf, Torsten; Schleipen, B.; Offermanns, C.; Velders, Martina; Laudenbach, U.; Diel, Patrick

doi:10.1016/j.toxlet.2008.11.006

Cited by 42 publications

(29 citation statements)

References 44 publications

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“…This finding suggests that bupropion may be considered beneficial in managing the diseases where inflammation is thought to be patho-physiologically active, such as osteoporotic conditions. In the present study, body weights of the OVX rats were significantly increased compared to the sham operated rats, which is in agreement with earlier reports [46][47][48] . It is well documented that estrogen deficiency in menopause is associated with a progressive increase in weight and redistribution of body fats.…”

Section: Effects On Bone Histopathologysupporting

confidence: 94%

“…Estrogen is the main sex hormone produced in females by the ovaries [52] , which induces uterine growth. Present data revealed a significant decrease in uterine weight and an increase in body weight ratio in estrogen deficient animals, which is in agreement with earlier studies [39,46] . The decline in the uterine weight ensured the success of the ovariectomy [52] .…”

Section: Effects On Bone Histopathologysupporting

confidence: 93%

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The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model

et al. 2014

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Aim: depression is a risk factor for impaired bone mass and micro-architecture, but several antidepressants were found to increase the incidence of osteoporotic fractures. in the present study we used ovariectomized (oVX) rats as a model of osteoporosis to investigate the effects of the antidepressant bupropion on the femoral bones. Methods: oVX animals were treated with bupropion (30, 60 mg·kg -1 ·d -1 ) for six weeks. bone turnover biomarkers (urinary dPd/Cr ratio, serum BALP, OC, TRAcP 5b, CTX and sRANKL levels) and inflammatory cytokines (TNF-α, IL-1β and IL-6) were determined using ELISA. inductively coupled plasma mass spectroscopy (iCP-Ms) was used to determine the femoral bone mineral concentrations. The cortical and trabecular morphometric parameters of femoral bones were determined using micro-CT scan and histopathology. Results: In OVX rats, the levels of bone turnover biomarkers and inflammatory cytokines were significantly elevated and femoral bone Ca 2+ and Po 4 3-concentrations were significantly reduced. Moreover, cortical and trabecular morphometric parameters and histopathology of femoral bones were severely altered by ovariectomy. bupropion dose-dependently inhibited the increases in bone turnover biomarkers and inflammatory cytokines. OVX rats treated with the high dose of bupropion showed normal mineral concentrations in femoral bones. The altered morphometric parameters and histopathology of femoral bones were markedly attenuated by the treatment. Conclusion: bupropion exerts osteo-protective action in oVX rats through suppressing osteoclastogenesis-inducing factors and inflammation, which stabilize the osteoclasts and decrease bone matrix degradation or resorption.

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Section: Effects On Bone Histopathologysupporting

confidence: 94%

Section: Effects On Bone Histopathologysupporting

confidence: 93%

The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model

et al. 2014

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“…In the present study on female rats with normal estrogen levels, both low and high doses of genistein treatment were observed to induce significant increase in BMD, BMC and bone volume, as well as inducing thicker and larger trabecular bone in the mandibular subchondral bone. These results were consistent with the above-mentioned studies on appendicular bone or vertebrae [17,18,20,[30][31][32][33][34][35][36] , indicating that the effect of genistein on mandibular subchondral bone is similar to that on appendicular bone or vertebrae. Similar to the biphasic effects of estrogen depending on dosage [37] , genistein was also observed to have a biphasic cell proliferative response, stimulation at low concentrations and inhibition at high concentrations [38][39][40] .…”

Section: Effect Of Er Silencing On the Effect Of Genistein Treatmentsupporting

confidence: 91%

“…ERα and ERβ siRNA were able to knockdown 72% and 80% of ER expression, respectively. As shown in Figure 6 and [18,20,[30][31][32][33][34][35][36] . Similarly, oral administration of genistein (54 mg·kg -1 ·d -1 ) increased BMD of the spine and hip in osteopenic postmenopausal Caucasian women with an observation period of 24 months [17] .…”

Section: Effect Of Er Silencing On the Effect Of Genistein Treatmentmentioning

confidence: 99%

Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone

Xing

Wang

et al. 2011

Acta Pharmacol Sin

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Aim: To investigate the effect of genistein on bone homeostasis in mandibular subchondral bone of rats. Methods: Female SD rats were administered with genistein (10 and 50 mg/kg) or placebo by oral gavage for 6 weeks. Then the animals were sacrificed, and histomorphology and micro-structure of mandibular condyle were examined using HE staining and micro-CT analysis, respectively. The expression levels of alkaline phosphatase (ALP), osteocalcin (OC), osteoprotegerin (OPG), the receptor activator of nuclear factor κB ligand (RANKL) and estrogen receptors (ERs) in mandibular condyle were detected using real-time PCR. Cultured osteoblasts were prepared from rat mandibular condyle for in in vitro study. The cells were treated with genistein (10 -7 or 10 -4 mol/L) for 48 h. The expression of the bone homeostasis-associated factors and estrogen receptors (ERs) was detected using realtime PCR, and ER silencing was performed. Results: At both the low-and high-doses, genistein significantly increased the bone mineral density (BMD) and bone volume, and resulted in thicker subchondral trabecular bone in vivo. In both in vivo and in vitro study, the low-dose genistein significantly increased the expression of ALP, OC and OPG, but decreased the expression of RANKL and the RANKL/OPG ratio. The high-dose genistein decreased the expression of all these bone homeostasis-associated factors. Both the low and high doses of genistein significantly increased the expression of ERβ, while ERα expression was increased by the low dose genistein and decreased by the high dose genistein. ERβ silencing abrogated most of the effects of genistein treatment. Conclusion: In rat mandibular condylar subchondral bone, low-dose genistein increases bone formation and inhibit bone resorption, while excess genistein inhibits both bone formation and resorption. The effects of genistein were predominantly mediated through ERβ.

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“…An increase in ALP serum levels, the most widely used biochemical bone turnover marker [25], was observed in OVX rats [26,27]. Furthermore, an increase in serum levels of telopeptides of collagen type I (CTx), which correlate with bone-resorption, with high levels indicating excessive osteoclastic activity and bone formation marker osteocalcin (OC) were observed in OVX rats, and these results were supported by Hertrampf et al [28]. Although we did not determine the 3D architecture of trabecular bone within the distal metaphyseal femur region, eight weeks of treatment with high-dose PTIF significantly decreased the BMD loss in the femur, which was reflected by the decrease in ALP, CTx and OC serum levels compared to the OVX-control group; this effect may have been due to decreased bone resorption.…”

Section: Discussionmentioning

confidence: 75%

Effects of Dietary Isoflavones from Puerariae Radix on Lipid and Bone Metabolism in Ovariectomized Rats

Lim

Kim

2013

Nutrients

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Puerariae radix, the dried root of Pueraria lobata Ohwi, is one of the earliest and most important edible crude herbs used for various medical purposes in oriental medicine. This study evaluated the metabolic effects of total isoflavones from P. lobata (PTIF) in ovariectomized (OVX) rats. The OVX rats were divided into four groups treated with distilled water, 17β-estradiol (E2 10 μg/kg, once daily, i.p.) and PTIF (30 and 100 mg/kg, once daily, p.o.) for eight weeks. The treatments with high-dose PTIF significantly decreased the bone mineral density (BMD) loss in the femur and inhibited the increase in body weight and lipoprotein levels compared to the OVX-control group without elevating the serum levels of the liver enzymes, aspartate aminotransferase (AST) and alanine transaminase (ALT). Furthermore, PTIF exhibits a hepatoprotective effect in OVX-induced hepatic steatosis, indicated with reduced hepatic lipid contents. Taken together, our findings suggest that PTIF may be useful for controlling lipid and bone metabolism, at least in OVX rats. Further research is necessary to determine whether PTIF will have the same effects in humans.

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Comparison of the bone protective effects of an isoflavone-rich diet with dietary and subcutaneous administrations of genistein in ovariectomized rats

Cited by 42 publications

References 44 publications

The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model

The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model

Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone

Effects of Dietary Isoflavones from Puerariae Radix on Lipid and Bone Metabolism in Ovariectomized Rats

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