The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model

Abstract

Aim: depression is a risk factor for impaired bone mass and micro-architecture, but several antidepressants were found to increase the incidence of osteoporotic fractures. in the present study we used ovariectomized (oVX) rats as a model of osteoporosis to investigate the effects of the antidepressant bupropion on the femoral bones. Methods: oVX animals were treated with bupropion (30, 60 mg·kg -1 ·d -1 ) for six weeks. bone turnover biomarkers (urinary dPd/Cr ratio, serum BALP, OC, TRAcP 5b, CTX and sRANKL levels) and inflammatory cytokines (TNF-α, IL-1β and IL-6) were determined using ELISA. inductively coupled plasma mass spectroscopy (iCP-Ms) was used to determine the femoral bone mineral concentrations. The cortical and trabecular morphometric parameters of femoral bones were determined using micro-CT scan and histopathology. Results: In OVX rats, the levels of bone turnover biomarkers and inflammatory cytokines were significantly elevated and femoral bone Ca 2+ and Po 4 3-concentrations were significantly reduced. Moreover, cortical and trabecular morphometric parameters and histopathology of femoral bones were severely altered by ovariectomy. bupropion dose-dependently inhibited the increases in bone turnover biomarkers and inflammatory cytokines. OVX rats treated with the high dose of bupropion showed normal mineral concentrations in femoral bones. The altered morphometric parameters and histopathology of femoral bones were markedly attenuated by the treatment. Conclusion: bupropion exerts osteo-protective action in oVX rats through suppressing osteoclastogenesis-inducing factors and inflammation, which stabilize the osteoclasts and decrease bone matrix degradation or resorption.

Osteoprotective Effect of Echinocystic Acid, a Triterpone Component from Eclipta prostrata, in Ovariectomy-Induced Osteoporotic Rats

“…Pro-infammatory cytokines (IL-1β, IL-6, IL-8 and TNF-α) play an important role in regulating bone resorption, while estrogen deficiency always leads to remarkable increase in pro-iflammatory cytokines [ 2 ]. In the study, the serum levels of IL-1β and TNF-α were much higher in OVX group, which is consistent with other studies [ 40 , 41 ]. EA has been shown to display an anti-inflammatory effect in different models of chronic inflammation in mice through the down-regulation of pro-inflammatory cytokines such as IL-1β, IL-18, TNF-α [ 16 , 17 , 18 ].…”

The participation of fibroblast growth factor 23 (FGF23) in the progression of osteoporosis via JAK/STAT pathway

“…Besides, TRAP is a representative enzyme of osteoclast marker . Previous studies have demonstrated that serum levels of the bone resorption biomarkers, such as BLAP, OC, TRAP, and CTX, were significantly elevated in the OVX group compared to the sham operated group . Our studies revealed that AG490 promoted OVX‐induced increase in the osteocalcin, ALP, BALP, TRAP, and CTX‐I levels.…”

Effects of tanshinol on markers of bone turnover in ovariectomized rats and osteoblast cultures

“…In OVX rats, a significant increase of serum and bone ALP, OCN and TRACP have been recorded [22, 23]. ALP and TRACP are respectively secreted by osteoblasts and osteoclasts, ALP is essential for bone mineralization while TRACP, especially TRACP-5b, is correlates with resorptive activity [24, 25].…”